LOCUS       sequence-192967-       10982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 gap repair vector - ZeoR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10982)
  TITLE     Ellis Lab Yeast CRISPR Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWS176
FEATURES             Location/Qualifiers
     source          1..10982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4879..4899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4903..4923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             5833..5844
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5851..6564
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6576..6647
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6663..6690
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        6704..6779
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      8013..8210
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      8215..9197
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(9255..10070)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9255..10070)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9978..9997
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10062..10081
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10295..10883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10784..10803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagactg aactggccga taattgcaga cgaacggtga
       61 gtaaggaaag agtgaggaac tatcgcatac ctgcatttaa agatgccgat ttgggcgcga
      121 atcctttatt ttggcttcac cctcatacta ttatcagggc cagaaaaagg aagtgtttcc
      181 ctccttcttg aattgatgtt accctcataa agcacgtggc ctcttatcga gaaagaaatt
      241 accgtcgctc gtgatttgtt tgcaaaaaga acaaaactga aaaaacccag acacgctcga
      301 cttcctgtca tcctattgat tgcagcttcc aatttcgtca cacaacaagg tcctagcgac
      361 ggctcacagg ttttgtaaca agcaatcgaa ggttctggaa tggcgggaaa gggtttagta
      421 ccacatgcta tgatgcccac tgtgatctcc agagcaaagt tcgttcgatc gtactgttac
      481 tctctctctt tcaaacagaa ttgtccgaat cgtgtgacaa caacagcctg ttctcacaca
      541 ctcttttctt ctaaccaagg gggtggttta gtttagtaga acctcgtgaa acttacattt
      601 acatatatat aaacttgcat aaattggtca atgcaagaaa tacatatttg gtcttttcta
      661 attcgtagtt tttcaagttc ttagatgctt tctttttctc ttttttacag atcatcaagg
      721 aagtaattat ctacttttta caacaaatat aaaacaagat ctatggacaa gaagtattct
      781 atcggactgg atatcgggac taatagcgtc gggtgggccg tgatcactga cgagtacaag
      841 gtgccctcta agaagttcaa ggtgctcggg aacaccgacc ggcattccat caagaaaaat
      901 ctgatcggag ctctcctctt tgattcaggg gaaaccgctg aagcaacccg cctcaagcgg
      961 actgctagac ggcggtacac caggaggaag aaccggattt gttaccttca agagatattc
     1021 tccaacgaaa tggcaaaggt cgacgacagc ttcttccata ggctggaaga atcattcctc
     1081 gtggaagagg ataagaagca tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg
     1141 gcctatcacg agaaataccc aaccatctac catcttcgca aaaagctggt ggactcaacc
     1201 gacaaggcag acctccggct tatctacctg gccctggccc acatgatcaa gttcagaggc
     1261 cacttcctga tcgagggcga cctcaatcct gacaatagcg atgtggataa actgttcatc
     1321 cagctggtgc agacttacaa ccagctcttt gaagagaacc ccatcaatgc aagcggagtc
     1381 gatgccaagg ccattctgtc agcccggctg tcaaagagcc gcagacttga gaatcttatc
     1441 gctcagctgc cgggtgaaaa gaaaaatgga ctgttcggga acctgattgc tctttcactt
     1501 gggctgactc ccaatttcaa gtctaatttc gacctggcag aggatgccaa gctgcaactg
     1561 tccaaggaca cctatgatga cgatctcgac aacctcctgg cccagatcgg tgaccaatac
     1621 gccgaccttt tccttgctgc taagaatctt tctgacgcca tcctgctgtc tgacattctc
     1681 cgcgtgaaca ctgaaatcac caaggcccct ctttcagctt caatgattaa gcggtatgat
     1741 gagcaccacc aggacctgac cctgcttaag gcactcgtcc ggcagcagct tccggagaag
     1801 tacaaggaaa tcttctttga ccagtcaaag aatggatacg ccggctacat cgacggaggt
     1861 gcctcccaag aggaatttta taagtttatc aaacctatcc ttgagaagat ggacggcacc
     1921 gaagagctcc tcgtgaaact gaatcgggag gatctgctgc ggaagcagcg cactttcgac
     1981 aatgggagca ttccccacca gatccatctt ggggagcttc acgccatcct tcggcgccaa
     2041 gaggacttct acccctttct taaggacaac agggagaaga ttgagaaaat tctcactttc
     2101 cgcatcccct actacgtggg acccctcgcc agaggaaata gccggtttgc ttggatgacc
     2161 agaaagtcag aagaaactat cactccctgg aacttcgaag aggtggtgga caagggagcc
     2221 agcgctcagt cattcatcga acggatgact aacttcgata agaacctccc caatgagaag
     2281 gtcctgccga aacattccct gctctacgag tactttaccg tgtacaacga gctgaccaag
     2341 gtgaaatatg tcaccgaagg gatgaggaag cccgcattcc tgtcaggcga acaaaagaag
     2401 gcaattgtgg accttctgtt caagaccaat agaaaggtga ccgtgaagca gctgaaggag
     2461 gactatttca agaaaattga atgcttcgac tctgtggaga ttagcggggt cgaagatcgg
     2521 ttcaacgcaa gcctgggtac ttaccatgat ctgcttaaga tcatcaagga caaggatttt
     2581 ctggacaatg aggagaacga ggacatcctt gaggacattg tcctgactct cactctgttc
     2641 gaggaccggg aaatgatcga ggagaggctt aagacctacg cccatctgtt cgacgataaa
     2701 gtgatgaagc aacttaaacg gagaagatat accggatggg gacgccttag ccgcaaactc
     2761 atcaacggaa tccgggacaa acagagcgga aagaccattc ttgatttcct taagagcgac
     2821 ggattcgcta atcgcaactt catgcaactt atccatgatg attccctgac ctttaaggag
     2881 gacatccaga aggcccaagt gtctggacaa ggtgactcac tgcacgagca tatcgcaaat
     2941 ctggctggtt cacccgctat taagaagggt attctccaga ccgtgaaagt cgtggacgag
     3001 ctggtcaagg tgatgggtcg ccataaacca gagaacattg tcatcgagat ggccagggaa
     3061 aaccagacta cccagaaggg acagaagaac agcagggagc ggatgaaaag aattgaggaa
     3121 gggattaagg agctcgggtc acagatcctt aaagagcacc cggtggaaaa cacccagctt
     3181 cagaatgaga agctctatct gtactacctt caaaatggac gcgatatgta tgtggaccaa
     3241 gagcttgata tcaacaggct ctcagactac gacgtggacc atatcgtccc tcagagcttc
     3301 ctcaaagacg actcaattga caataaggtg ctgactcgct cagacaagaa ccggggaaag
     3361 tcagataacg tgccctcaga ggaagtcgtg aaaaagatga agaactattg gcgccagctt
     3421 ctgaacgcaa agctgatcac tcagcggaag ttcgacaatc tcactaaggc tgagaggggc
     3481 ggactgagcg aactggacaa agcaggattc attaaacggc aacttgtgga gactcggcag
     3541 attactaaac atgtcgccca aatccttgac tcacgcatga ataccaagta cgacgaaaac
     3601 gacaaactta tccgcgaggt gaaggtgatt accctgaagt ccaagctggt cagcgatttc
     3661 agaaaggact ttcaattcta caaagtgcgg gagatcaata actatcatca tgctcatgac
     3721 gcatatctga atgccgtggt gggaaccgcc ctgatcaaga agtacccaaa gctggaaagc
     3781 gagttcgtgt acggagacta caaggtctac gacgtgcgca agatgattgc caaatctgag
     3841 caggagatcg gaaaggccac cgcaaagtac ttcttctaca gcaacatcat gaatttcttc
     3901 aagaccgaaa tcacccttgc aaacggtgag atccggaaga ggccgctcat cgagactaat
     3961 ggggagactg gcgaaatcgt gtgggacaag ggcagagatt tcgctaccgt gcgcaaagtg
     4021 ctttctatgc ctcaagtgaa catcgtgaag aaaaccgagg tgcaaaccgg aggcttttct
     4081 aaggaatcaa tcctccccaa gcgcaactcc gacaagctca ttgcaaggaa gaaggattgg
     4141 gaccctaaga agtacggcgg attcgattca ccaactgtgg cttattctgt cctggtcgtg
     4201 gctaaggtgg aaaaaggaaa gtctaagaag ctcaagagcg tgaaggaact gctgggtatc
     4261 accattatgg agcgcagctc cttcgagaag aacccaattg actttctcga agccaaaggt
     4321 tacaaggaag tcaagaagga ccttatcatc aagctcccaa agtatagcct gttcgaactg
     4381 gagaatgggc ggaagcggat gctcgcctcc gctggcgaac ttcagaaggg taatgagctg
     4441 gctctcccct ccaagtacgt gaatttcctc taccttgcaa gccattacga gaagctgaag
     4501 gggagccccg aggacaacga gcaaaagcaa ctgtttgtgg agcagcataa gcattatctg
     4561 gacgagatca ttgagcagat ttccgagttt tctaaacgcg tcattctcgc tgatgccaac
     4621 ctcgataaag tccttagcgc atacaataag cacagagaca aaccaattcg ggagcaggct
     4681 gagaatatca tccacctgtt caccctcacc aatcttggtg cccctgccgc attcaagtac
     4741 ttcgacacca ccatcgaccg gaaacgctat acctccacca aagaagtgct ggacgccacc
     4801 ctcatccacc agagcatcac cggactttac gaaactcgga ttgacctctc acagctcgga
     4861 ggggatgagg gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     4921 gtataaggat cctaactcga gattgaattg aattgaaatc gatagatcaa tttttttctt
     4981 ttctctttcc ccatccttta cgctaaaata atagtttatt ttattttttg aatatttttt
     5041 atttatatac gtatatatag actattattt atcttttaat gattattaag atttttatta
     5101 aaaaaaaatt cgctcctctt ttaatgcctt tatgcagttt ttttttccca ttcgatattt
     5161 ctatgtgctg ccaagatatc aacaacagag gacatatgcc ctacctccat gatctactga
     5221 cgtccctgag gctgcaattc atgtaatggg gcagtatccg cggcaagtcc tagtgcaatg
     5281 gcggtttttt accctcgttc tgaagaagag gcgacgcggg tgcggtcatc actaatgtgg
     5341 aaattggtaa gactctcggg cctccgcctt taggcggtgc ttactctttc ataaaggggc
     5401 tgttagttat gcaacgatgt gcttcagtat tacatttttt gccttcaacg ccttgattgt
     5461 tctatttttg ctaataataa atctatttca tcggactaaa agtccattag ttgtaagcgg
     5521 atttagctca gttgggagag cgccagactg aagaaaaact tcggtcaagt catctggagg
     5581 tcctgtgttc gatccacaga attcgcagat ggccggcatg gtcccagcct cctcgctggc
     5641 gccggctggg caacaccttc gggtggcgaa tgggacttga gacggaaagt gaaacgtgat
     5701 ttcatgcgtc attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca
     5761 tttaatttat gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct
     5821 tagctactag agaaagagga gaaatactag atgcgtaaag gcgaagagct gttcactggt
     5881 gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt
     5941 ggcgagggtg aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact
     6001 ggtaaactgc cggttccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc
     6061 tttgctcgtt atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa
     6121 ggctatgtgc aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg
     6181 gaagtgaaat ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt
     6241 aaagaggacg gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt
     6301 tacatcaccg ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac
     6361 gtggaggatg gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat
     6421 ggtcctgttc tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat
     6481 ccgaacgaga aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg
     6541 catggtatgg atgaactgta caaatgacca ggcatcaaat aaaacgaaag gctcagtcga
     6601 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     6661 ctggctcacc ttcgggtggg cctttctgcg tttatacgtc tcagttttag agctagaaat
     6721 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     6781 ttttttattt tttgtcacta ttgttatgta aaatgccacc tctgacagta tggaacgcaa
     6841 acttctgtct agtggatagc tggacattcc aggcagtgcc tctgccgccg gacccctctc
     6901 gtgattgggt agctggacat gcccttgtaa gatataacaa gagcctgcct gtctaatgat
     6961 ctcacggcga aagtcgggga gacagcagcg gctgcagaca ttataccgca acaacactaa
     7021 ggtgagataa ctccgtacat tgactacgcg ttcctctaga ccttacttga ccggatacag
     7081 tgtctttgac acgtttatgg gttacagcaa tcacatccaa gactggctat gcacgaagca
     7141 actcttgagt gttaaaatgt tgacccctgt atttgggatg cgggtagtag atgagtgcag
     7201 gatatcagca gttacagaga tgttacgaac cactagtgca ctgcagtaca gtttagcttg
     7261 cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca
     7321 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
     7381 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
     7441 attacggctc ctcgctccag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
     7501 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
     7561 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
     7621 aacataaaca aaaatggcta aattaacatc tgccgttcct gttttaacag ctagggatgt
     7681 tgcaggtgct gtagagtttt ggacagatag gttaggattc tcaagagact ttgttgagga
     7741 cgattttgct ggtgttgtca gggatgacgt tactttattt atctcagcag tccaagatca
     7801 agttgtccct gataatacat tggcttgggt ctgggtcagg ggtttagatg aattatatgc
     7861 tgaatggtca gaagttgtat ctacaaactt cagagatgct tctggtccag ctatgaccga
     7921 gattggtgaa cagccatggg gtagagaatt tgctttgaga gatccagctg gaaattgtgt
     7981 tcattttgtt gctgaagaac aagattaaag taactgacaa taaaaagatt cttgttttca
     8041 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
     8101 gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc
     8161 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg gagtaacgaa
     8221 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     8281 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     8341 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
     8401 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     8461 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     8521 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     8581 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     8641 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     8701 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     8761 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     8821 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     8881 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     8941 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     9001 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     9061 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     9121 gtttttggtt ttttgaaagt gcgtcatcag agcgcttttg gttttcaaaa gcgctctgaa
     9181 gttcctatac tttctagcta gagaatagga acttcccgag cggccgcgtg ttacaaccaa
     9241 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     9301 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     9361 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     9421 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     9481 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     9541 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     9601 ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa aggacaatta
     9661 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     9721 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     9781 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     9841 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     9901 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     9961 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
    10021 gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
    10081 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
    10141 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
    10201 gctgagttga aggatcagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10441 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10981 gt
//
