LOCUS       sequence-192971-       11026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary construct of nuclear mCherry with sfGFP11 tag for plant 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Park E, Lee HY, Woo J, Choi D, Dinesh-Kumar SP
  TITLE     Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type
            III Secretion Using Split Fluorescent Protein Fragments.
  JOURNAL   Plant Cell. 2017 Jul;29(7):1571-1584. doi: 10.1105/tpc.17.00047. 
            Epub 2017 Jun 14.
  PUBMED    28619883
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00047"; journalName: "Plant Cell"; date: "2017-07"; 
            volume: "29"; issue: "7"; pages: "1571-1584"
COMMENT     Sequence Label: PEP110E
FEATURES             Location/Qualifiers
     source          1..11026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      58..310
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    332..356
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1657..2286
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2715..3788
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3854..4048
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4284..4306)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4392..4532
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4447..4466
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4547..4564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4718..5306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4798..4817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5393..6187)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6612..6636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6714..6888
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6929..7954)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8021..8697)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8064..8084)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10127..10147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10151..10855
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     10561..10580
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             10865..10894
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(10913..10929)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10913..10929)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             10961..11008
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
       61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
      121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
      181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
      241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
      301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
      361 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
      421 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
      481 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
      541 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
      601 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
      661 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
      721 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
      781 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
      841 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
      901 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
      961 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     1021 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     1081 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     1141 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     1201 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     1261 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     1321 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     1381 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     1441 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     1501 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     1561 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     1621 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     1681 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     1741 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     1801 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     1861 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     1921 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     1981 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     2041 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     2101 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     2161 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     2221 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     2281 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     2341 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     2401 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     2461 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     2521 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     2581 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     2641 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
     2701 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     2761 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     2821 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     2881 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     2941 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     3001 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     3061 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     3121 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     3181 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     3241 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     3301 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     3361 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     3421 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     3481 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     3541 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     3601 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     3661 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     3721 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     3781 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     3841 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     3901 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     3961 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     4021 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     4081 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     4141 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     4201 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     4261 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4321 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4381 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     4441 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     4501 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     4561 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4621 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4681 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4741 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4801 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4861 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4921 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4981 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5041 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5101 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5161 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5221 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5281 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5341 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
     5401 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
     5461 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
     5521 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
     5581 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
     5641 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
     5701 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
     5761 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
     5821 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
     5881 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
     5941 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
     6001 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
     6061 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
     6121 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
     6181 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
     6241 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
     6301 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
     6361 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     6421 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     6481 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     6541 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     6601 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
     6661 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
     6721 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
     6781 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga
     6841 accctaattc ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc
     6901 gatcgacaga tcccggtcgg catctactct atttctttgc cctcggacga gtgctggggc
     6961 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     7021 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     7081 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     7141 caagaccaat gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc
     7201 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     7261 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     7321 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     7381 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     7441 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     7501 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     7561 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta
     7621 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg
     7681 agatgcaata ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga
     7741 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     7801 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     7861 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     7921 cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg gatctgcgaa
     7981 agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
     8041 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
     8101 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     8161 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     8221 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
     8281 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     8341 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     8401 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttcaca tcaatccact
     8461 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     8521 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
     8581 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     8641 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     8701 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     8761 tccaccatgt tgggcccggc gcgccgaatt cccggggatc cgtcgaggat caggatattc
     8821 ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag gaccggataa
     8881 gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc ttgttacatt
     8941 gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa atatggacca
     9001 ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc gagtcaccaa
     9061 accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc attatcctat
     9121 gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag aaatggataa
     9181 tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact gattttataa
     9241 gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat aaagcacgaa
     9301 gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc aattattgcc
     9361 aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa aatataaatc
     9421 gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa ttgtggttgt
     9481 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
     9541 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
     9601 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
     9661 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
     9721 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
     9781 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
     9841 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
     9901 ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg
     9961 attagggttt cataaatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
    10021 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
    10081 tttgtcgatt aatctgagtt tttctgatta acagatgaag cttatgccaa agaaaaagcg
    10141 gaaagtcgga gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
    10201 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
    10261 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
    10321 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
    10381 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
    10441 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
    10501 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
    10561 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
    10621 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
    10681 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
    10741 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
    10801 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggcatg
    10861 catggagcaa aagctcattt ctgaagagga cttgaattcc tgcagcccgg gggatccact
    10921 agttctagag ctcggcgacg gcggcagcgg cggcggcagc cgggaccaca tggtgctgca
    10981 cgagtacgtg aacgccgccg gcatcacatg aagatctatc taaacg
//
