LOCUS       sequence-192974-       11218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Evi-1-Flag with GFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11218)
  TITLE     Perkins lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKAM-E-Flag-GFP
FEATURES             Location/Qualifiers
     source          1..11218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6538..6561
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     6995..7013
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      7092..7101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7098..7100,7101..7103,7104..7817)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7098..7100,7101..7103,7104..7814)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7143..7164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7404..7423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7751..7772
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7936..8569
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8698..8714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8698..8714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8711..8733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8722..8738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8746..8752,8753..8770,8771..8776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8791..8812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8929..8946)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9100..9685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9180..9199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9856..10647,10648..10716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10479..10498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10717..10821)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10869..11003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10906..10925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10960..10979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatcccagc atgagatcca aaaagagtga agaggacccg
     3421 cacgaaccca tggcgcctga catccacgaa gaacggcagc accgctgtga ggactgtgac
     3481 cagctctttg aatccaaggc agagctagcc gatcaccaga agttcccatg cagcacacct
     3541 cactcggcct tctccatggt ggaggaggac ttgcaacaaa acctggagag tgagagcgat
     3601 ctccgagaga tccatggcaa ccaggactgt aaggaatgtg accgagtttt ccccgatctg
     3661 caaagcttgg agaagcacat gctgtcacat actgaggaga gggaatacaa gtgtgatcag
     3721 tgtcccaagg catttaactg gaagtccaat ttaattcgcc accagatgtc acatgacagt
     3781 ggaaagcact atgagtgtga aaactgtgcc aaggttttca cggaccctag caaccttcag
     3841 cgacacattc gatctcagca tgttggtgcc cgggctcatg cttgccccga gtgtggtaaa
     3901 acatttgcca cttcgtcagg cctcaaacag cacaagcaca tccacagcag tgtgaagccc
     3961 tttatctgtg aggtctgcca taaatcctat actcagtttt caaacctttg tcgtcataag
     4021 cgcatgcatg ctgattgcag aacccaaatc aagtgcaaag actgtggaca aatgttcagc
     4081 actacgtctt ccttaaataa acacaggagg ttttgtgagg gcaagaacca ttttgcggca
     4141 ggtggatttt ttggccaagg catttcactt cctggaaccc cagctatgga taaaacgtcc
     4201 atggttaata tgagtcatgc caacccgggc ctggctgact attttggcac caataggcat
     4261 cctgctggtc ttacctttcc aacagctcct ggattttcct ttagctttcc tggtctgttt
     4321 ccttcgggct tataccacag gcctcctttg atacccgcta gtcctcctgt taaaggatta
     4381 tcaagtaccg aacagtcaaa caaatgtcaa agtcccctct tgacacatcc tcagatactg
     4441 ccagctacac aggatatttt gaaggcacta tctaaacacc cacctgtagg ggacaataag
     4501 ccagtggaac tgctgcccga gaggtcctct gaagagaggc cccttgagaa aatcagtgac
     4561 cagtcagaga gtagtgacct tgatgatgtc agtacaccaa gtggcagtga cctggaaaca
     4621 acctcgggct ctgatctgga aagtgacctt gaaagtgata aagagaaatg taaagaaaat
     4681 ggtaaaatgt tcaaagacaa agtaagccct ctgcagaatc tggcttcgat aactaataag
     4741 aaagaacaca acaatcattc cgttttctca gcatctgtag aggagcaaag tgccgtgtca
     4801 ggagctgtga atgattctat aaaggctatt gcgtctattg ctgaaaaata ctttggttct
     4861 acaggactgg tggggctgca agacaaaaaa gttggagcgt taccttaccc ttccatgttt
     4921 cccctcccgt tttttccagc attctctcaa tcaatgtacc catttcctga tagagacttg
     4981 aggtcgttac ctttgaaaat ggagccccaa tcaccaagtg aagttaagaa actgcagaag
     5041 ggaagctctg agtccccttt tgacctcacc actaagagaa aggatgagaa gcccttgact
     5101 tcaggcccct cgaagccttc aggaacacca gccacaagcc aagaccagcc cctggatcta
     5161 agtatgggca gtaggggtag agccagtggg acaaagttga ctgagcctcg aaaaaaccat
     5221 gtgtttgggg aaaagaaagg aagcaacatg gatactaggc catcttcaga tggctccttg
     5281 cagcatgcca gacccactcc cttcttcatg gaccccattt atagagtaga gaaaagaaag
     5341 ttaactgacc cgcttgaagc tttgaaagaa aaatacttga gaccttctcc aggattcttg
     5401 tttcacccgc aaatgtcagc aattgagaac atggcagaaa agctggaaag cttcagcgcc
     5461 ctcaaacctg aggccagcga gctcctgcag tccgtgccct ccatgttcag cttccgagct
     5521 cctcccaaca ccctgccaga gaacctgctg cggaagggga aagagcgcta cacctgcagg
     5581 tactgtggca agatatttcc aaggtctgcg aacctaacac ggcacttgag aacccacaca
     5641 ggagagcaac cttacagatg caaatactgt gatagatcat tcagcatttc ttccaacctg
     5701 cagcgacatg tgcgcaacat ccacaacaag gagaagccat ttaagtgtca tttatgtgac
     5761 agatgttttg gtcaacaaac caatcttgac agacacctga agaaacatga gaacggcaac
     5821 atgtctggga cggcaacgtc ctcgcctcac tcagagctag aaagcgcagg cgcaatcctg
     5881 gatgacaaag aagatgcgta ctttacagag atccgcaatt tcatcgggaa cagcaaccat
     5941 ggtagccagt ctcctcggaa catggaagag aggatgaatg gcagtcactt caaggataaa
     6001 aaggctttgg caaccagcca aaattcagat ttattggatg atgaagaagt agaagatgag
     6061 gtgttgttgg atgaggagga tgaagacaat gatattcctg gaaagcccag aaaggagcta
     6121 ggggtgactc gtttagacga ggagatcccg gaggatgact acgaagaagc tggtgccctg
     6181 gagatgagct gtaaggcgtc cccggtgagg tataaagagg aagactataa atctggcctt
     6241 tctgctctag atcacataag gcacttcaca gatagcctca aaatgaggga aatggaagag
     6301 aatcaataca ctgacgctga gctgtcctcc attagttctt ctcatgtgcc agaggagctt
     6361 aaacagacgt tacacagaaa gtccaaatca caggcatatg ctatgatgtt gtcactgtct
     6421 gacaaggatt ccctccatcc cacctcccac agttcctcca acgtgtggca cagcatggca
     6481 agggctgcag cagaatccag tgccatccag tccataagcc atgtaggcag cggcgtagat
     6541 tacaaggatg acgacgataa gtaggcgaat tctaccgggt aggggaggcg cttttcccaa
     6601 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     6661 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     6721 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     6781 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtacc ctcgcagacg
     6841 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     6901 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     6961 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     7021 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     7081 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     7141 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     7201 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     7261 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     7321 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     7381 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     7441 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     7501 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     7561 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     7621 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7681 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7741 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7801 acgagctgta caagtaaagc ggccgcgtcg acgggccctc gacgggcccg cggaattcga
     7861 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7921 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7981 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     8041 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     8101 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     8161 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     8221 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     8281 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     8341 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     8401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8581 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8641 ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8701 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8761 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8821 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8881 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8941 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9001 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9061 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9121 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9181 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9241 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9301 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9361 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9421 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9481 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9541 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9601 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat
     9661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9721 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9781 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9841 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9901 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9961 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10021 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10081 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10141 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10201 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10261 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10321 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10381 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10441 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10501 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10561 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10621 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10681 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10741 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10801 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10861 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10981 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    11041 ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt actctaaacc
    11101 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    11161 aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
