LOCUS       sequence-192975-       11850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Evi-1-Flag with dual BFP and puro markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11850)
  TITLE     Perkins lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKAM-E-Flag-BFP
FEATURES             Location/Qualifiers
     source          1..11850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6538..6561
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6577..7076
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     7009..7028
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7116..7712
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7116..7135)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7612..7632
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7731..7784
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7785..8486
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             8568..9201
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9330..9346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9330..9346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9343..9365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9354..9370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9378..9384,9385..9402,9403..9408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9423..9444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9561..9578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9732..10317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9812..9831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10488..11279,11280..11348))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11111..11130
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11349..11453)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11501..11635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11538..11557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11592..11611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatcccagc atgagatcca aaaagagtga agaggacccg
     3421 cacgaaccca tggcgcctga catccacgaa gaacggcagc accgctgtga ggactgtgac
     3481 cagctctttg aatccaaggc agagctagcc gatcaccaga agttcccatg cagcacacct
     3541 cactcggcct tctccatggt ggaggaggac ttgcaacaaa acctggagag tgagagcgat
     3601 ctccgagaga tccatggcaa ccaggactgt aaggaatgtg accgagtttt ccccgatctg
     3661 caaagcttgg agaagcacat gctgtcacat actgaggaga gggaatacaa gtgtgatcag
     3721 tgtcccaagg catttaactg gaagtccaat ttaattcgcc accagatgtc acatgacagt
     3781 ggaaagcact atgagtgtga aaactgtgcc aaggttttca cggaccctag caaccttcag
     3841 cgacacattc gatctcagca tgttggtgcc cgggctcatg cttgccccga gtgtggtaaa
     3901 acatttgcca cttcgtcagg cctcaaacag cacaagcaca tccacagcag tgtgaagccc
     3961 tttatctgtg aggtctgcca taaatcctat actcagtttt caaacctttg tcgtcataag
     4021 cgcatgcatg ctgattgcag aacccaaatc aagtgcaaag actgtggaca aatgttcagc
     4081 actacgtctt ccttaaataa acacaggagg ttttgtgagg gcaagaacca ttttgcggca
     4141 ggtggatttt ttggccaagg catttcactt cctggaaccc cagctatgga taaaacgtcc
     4201 atggttaata tgagtcatgc caacccgggc ctggctgact attttggcac caataggcat
     4261 cctgctggtc ttacctttcc aacagctcct ggattttcct ttagctttcc tggtctgttt
     4321 ccttcgggct tataccacag gcctcctttg atacccgcta gtcctcctgt taaaggatta
     4381 tcaagtaccg aacagtcaaa caaatgtcaa agtcccctct tgacacatcc tcagatactg
     4441 ccagctacac aggatatttt gaaggcacta tctaaacacc cacctgtagg ggacaataag
     4501 ccagtggaac tgctgcccga gaggtcctct gaagagaggc cccttgagaa aatcagtgac
     4561 cagtcagaga gtagtgacct tgatgatgtc agtacaccaa gtggcagtga cctggaaaca
     4621 acctcgggct ctgatctgga aagtgacctt gaaagtgata aagagaaatg taaagaaaat
     4681 ggtaaaatgt tcaaagacaa agtaagccct ctgcagaatc tggcttcgat aactaataag
     4741 aaagaacaca acaatcattc cgttttctca gcatctgtag aggagcaaag tgccgtgtca
     4801 ggagctgtga atgattctat aaaggctatt gcgtctattg ctgaaaaata ctttggttct
     4861 acaggactgg tggggctgca agacaaaaaa gttggagcgt taccttaccc ttccatgttt
     4921 cccctcccgt tttttccagc attctctcaa tcaatgtacc catttcctga tagagacttg
     4981 aggtcgttac ctttgaaaat ggagccccaa tcaccaagtg aagttaagaa actgcagaag
     5041 ggaagctctg agtccccttt tgacctcacc actaagagaa aggatgagaa gcccttgact
     5101 tcaggcccct cgaagccttc aggaacacca gccacaagcc aagaccagcc cctggatcta
     5161 agtatgggca gtaggggtag agccagtggg acaaagttga ctgagcctcg aaaaaaccat
     5221 gtgtttgggg aaaagaaagg aagcaacatg gatactaggc catcttcaga tggctccttg
     5281 cagcatgcca gacccactcc cttcttcatg gaccccattt atagagtaga gaaaagaaag
     5341 ttaactgacc cgcttgaagc tttgaaagaa aaatacttga gaccttctcc aggattcttg
     5401 tttcacccgc aaatgtcagc aattgagaac atggcagaaa agctggaaag cttcagcgcc
     5461 ctcaaacctg aggccagcga gctcctgcag tccgtgccct ccatgttcag cttccgagct
     5521 cctcccaaca ccctgccaga gaacctgctg cggaagggga aagagcgcta cacctgcagg
     5581 tactgtggca agatatttcc aaggtctgcg aacctaacac ggcacttgag aacccacaca
     5641 ggagagcaac cttacagatg caaatactgt gatagatcat tcagcatttc ttccaacctg
     5701 cagcgacatg tgcgcaacat ccacaacaag gagaagccat ttaagtgtca tttatgtgac
     5761 agatgttttg gtcaacaaac caatcttgac agacacctga agaaacatga gaacggcaac
     5821 atgtctggga cggcaacgtc ctcgcctcac tcagagctag aaagcgcagg cgcaatcctg
     5881 gatgacaaag aagatgcgta ctttacagag atccgcaatt tcatcgggaa cagcaaccat
     5941 ggtagccagt ctcctcggaa catggaagag aggatgaatg gcagtcactt caaggataaa
     6001 aaggctttgg caaccagcca aaattcagat ttattggatg atgaagaagt agaagatgag
     6061 gtgttgttgg atgaggagga tgaagacaat gatattcctg gaaagcccag aaaggagcta
     6121 ggggtgactc gtttagacga ggagatcccg gaggatgact acgaagaagc tggtgccctg
     6181 gagatgagct gtaaggcgtc cccggtgagg tataaagagg aagactataa atctggcctt
     6241 tctgctctag atcacataag gcacttcaca gatagcctca aaatgaggga aatggaagag
     6301 aatcaataca ctgacgctga gctgtcctcc attagttctt ctcatgtgcc agaggagctt
     6361 aaacagacgt tacacagaaa gtccaaatca caggcatatg ctatgatgtt gtcactgtct
     6421 gacaaggatt ccctccatcc cacctcccac agttcctcca acgtgtggca cagcatggca
     6481 agggctgcag cagaatccag tgccatccag tccataagcc atgtaggcag cggcgtagat
     6541 tacaaggatg acgacgataa gtaggcgaat tctaccgggt aggggaggcg cttttcccaa
     6601 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     6661 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     6721 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     6781 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     6841 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     6901 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     6961 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     7021 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     7081 gcatccatct agatctcgag cagctgaagc ttaccatgac cgagtacaag cccacggtgc
     7141 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7201 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7261 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7321 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7381 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7441 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7501 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7561 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7621 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7681 gcacctggtg catgacccgc aagcccggtg ccggcggcgg gtccggagga gagggcagag
     7741 gaagtctcct aacatgcggt gacgtggagg agaatcctgg cccaatgagc gagctgatta
     7801 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
     7861 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     7921 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
     7981 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
     8041 agggcttcac atgggagaga gtcaccacat acgaggacgg gggcgtgctg accgctaccc
     8101 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     8161 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
     8221 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
     8281 gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga
     8341 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     8401 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
     8461 gcaaactggg gcacaagctt aattgagcgg ccgctaggta cctttaagac caatgactta
     8521 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     8581 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     8641 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     8701 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     8761 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     8821 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     8881 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     8941 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     9001 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9061 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9121 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9181 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     9241 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg
     9301 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9361 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9421 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9481 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9541 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9601 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9721 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    10021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10141 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    10201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10261 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10321 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10381 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10441 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10501 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10561 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10621 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10681 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10741 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10801 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10861 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10921 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10981 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    11041 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    11101 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    11161 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    11221 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11281 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11341 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11401 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11461 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
    11521 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11581 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    11641 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11701 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11761 aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat
    11821 ttgttaaata tgtactacaa acttagtagt
//
