LOCUS       sequence-192976-       12411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12411)
  TITLE     Perkins lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKAM-ME-Flag-BFP
FEATURES             Location/Qualifiers
     source          1..12411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7099..7122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        7138..7637
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     7570..7589
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7677..8273
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7677..7696)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8173..8193
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             8292..8345
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8346..9047
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             9129..9762
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9891..9907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9891..9907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9904..9926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9915..9931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9939..9945,9946..9963,9964..9969))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9984..10005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10122..10139)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10293..10878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10373..10392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11049..11840,11841..11909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11672..11691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11910..12014)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12062..12196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12099..12118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12153..12172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatcccagc atgagatcca aaggcagggc aaggaaactg
     3421 gccacaagta atgagtgtgc ctatggcaac tatcctgaaa tacctttgga agaaatgcca
     3481 gatgctgatg cagatgggat aaccagtgtt ccctccctcc acattcaaga gccatgctct
     3541 cctgcgacgt ccagtgagtc atttactcct aaggagggct cgccatacaa agctcccatc
     3601 tacatccctg atgacatccc tattcctgat gagtttgagc ttcgagagtc aactatgcct
     3661 ggagcaggac ttggaatatg gaccaaaagg aagattgaaa taggcgaaaa gtttgggcca
     3721 tacatgggag agcagagatc agacctgaaa gattccagct atggatggga gatcttagat
     3781 gagttttgca atgtgaagtt ctgcatagat gccagtcaac cagatgtagg aagctggctc
     3841 aagtacatca gattcgctgg ctgctatgat cagcacaacc ttgttgcatg ccagataaat
     3901 gatcagatat tctaccgagt agtcgcagac attgcgcctg gggaagagct cttgctgttc
     3961 atgaagagtg aagaggaccc gcacgaaccc atggcgcctg acatccacga agaacggcag
     4021 caccgctgtg aggactgtga ccagctcttt gaatccaagg cagagctagc cgatcaccag
     4081 aagttcccat gcagcacacc tcactcggcc ttctccatgg tggaggagga cttgcaacaa
     4141 aacctggaga gtgagagcga tctccgagag atccatggca accaggactg taaggaatgt
     4201 gaccgagttt tccccgatct gcaaagcttg gagaagcaca tgctgtcaca tactgaggag
     4261 agggaataca agtgtgatca gtgtcccaag gcatttaact ggaagtccaa tttaattcgc
     4321 caccagatgt cacatgacag tggaaagcac tatgagtgtg aaaactgtgc caaggttttc
     4381 acggacccta gcaaccttca gcgacacatt cgatctcagc atgttggtgc ccgggctcat
     4441 gcttgccccg agtgtggtaa aacatttgcc acttcgtcag gcctcaaaca gcacaagcac
     4501 atccacagca gtgtgaagcc ctttatctgt gaggtctgcc ataaatccta tactcagttt
     4561 tcaaaccttt gtcgtcataa gcgcatgcat gctgattgca gaacccaaat caagtgcaaa
     4621 gactgtggac aaatgttcag cactacgtct tccttaaata aacacaggag gttttgtgag
     4681 ggcaagaacc attttgcggc aggtggattt tttggccaag gcatttcact tcctggaacc
     4741 ccagctatgg ataaaacgtc catggttaat atgagtcatg ccaacccggg cctggctgac
     4801 tattttggca ccaataggca tcctgctggt cttacctttc caacagctcc tggattttcc
     4861 tttagctttc ctggtctgtt tccttcgggc ttataccaca ggcctccttt gatacccgct
     4921 agtcctcctg ttaaaggatt atcaagtacc gaacagtcaa acaaatgtca aagtcccctc
     4981 ttgacacatc ctcagatact gccagctaca caggatattt tgaaggcact atctaaacac
     5041 ccacctgtag gggacaataa gccagtggaa ctgctgcccg agaggtcctc tgaagagagg
     5101 ccccttgaga aaatcagtga ccagtcagag agtagtgacc ttgatgatgt cagtacacca
     5161 agtggcagtg acctggaaac aacctcgggc tctgatctgg aaagtgacct tgaaagtgat
     5221 aaagagaaat gtaaagaaaa tggtaaaatg ttcaaagaca aagtaagccc tctgcagaat
     5281 ctggcttcga taactaataa gaaagaacac aacaatcatt ccgttttctc agcatctgta
     5341 gaggagcaaa gtgccgtgtc aggagctgtg aatgattcta taaaggctat tgcgtctatt
     5401 gctgaaaaat actttggttc tacaggactg gtggggctgc aagacaaaaa agttggagcg
     5461 ttaccttacc cttccatgtt tcccctcccg ttttttccag cattctctca atcaatgtac
     5521 ccatttcctg atagagactt gaggtcgtta cctttgaaaa tggagcccca atcaccaagt
     5581 gaagttaaga aactgcagaa gggaagctct gagtcccctt ttgacctcac cactaagaga
     5641 aaggatgaga agcccttgac ttcaggcccc tcgaagcctt caggaacacc agccacaagc
     5701 caagaccagc ccctggatct aagtatgggc agtaggggta gagccagtgg gacaaagttg
     5761 actgagcctc gaaaaaacca tgtgtttggg gaaaagaaag gaagcaacat ggatactagg
     5821 ccatcttcag atggctcctt gcagcatgcc agacccactc ccttcttcat ggaccccatt
     5881 tatagagtag agaaaagaaa gttaactgac ccgcttgaag ctttgaaaga aaaatacttg
     5941 agaccttctc caggattctt gtttcacccg caaatgtcag caattgagaa catggcagaa
     6001 aagctggaaa gcttcagcgc cctcaaacct gaggccagcg agctcctgca gtccgtgccc
     6061 tccatgttca gcttccgagc tcctcccaac accctgccag agaacctgct gcggaagggg
     6121 aaagagcgct acacctgcag gtactgtggc aagatatttc caaggtctgc gaacctaaca
     6181 cggcacttga gaacccacac aggagagcaa ccttacagat gcaaatactg tgatagatca
     6241 ttcagcattt cttccaacct gcagcgacat gtgcgcaaca tccacaacaa ggagaagcca
     6301 tttaagtgtc atttatgtga cagatgtttt ggtcaacaaa ccaatcttga cagacacctg
     6361 aagaaacatg agaacggcaa catgtctggg acggcaacgt cctcgcctca ctcagagcta
     6421 gaaagcgcag gcgcaatcct ggatgacaaa gaagatgcgt actttacaga gatccgcaat
     6481 ttcatcggga acagcaacca tggtagccag tctcctcgga acatggaaga gaggatgaat
     6541 ggcagtcact tcaaggataa aaaggctttg gcaaccagcc aaaattcaga tttattggat
     6601 gatgaagaag tagaagatga ggtgttgttg gatgaggagg atgaagacaa tgatattcct
     6661 ggaaagccca gaaaggagct aggggtgact cgtttagacg aggagatccc ggaggatgac
     6721 tacgaagaag ctggtgccct ggagatgagc tgtaaggcgt ccccggtgag gtataaagag
     6781 gaagactata aatctggcct ttctgctcta gatcacataa ggcacttcac agatagcctc
     6841 aaaatgaggg aaatggaaga gaatcaatac actgacgctg agctgtcctc cattagttct
     6901 tctcatgtgc cagaggagct taaacagacg ttacacagaa agtccaaatc acaggcatat
     6961 gctatgatgt tgtcactgtc tgacaaggat tccctccatc ccacctccca cagttcctcc
     7021 aacgtgtggc acagcatggc aagggctgca gcagaatcca gtgccatcca gtccataagc
     7081 catgtaggca gcggcgtaga ttacaaggat gacgacgata agtaggcgaa ttctaccggg
     7141 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     7201 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     7261 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     7321 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     7381 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     7441 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     7501 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     7561 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     7621 catctccggg cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga
     7681 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     7741 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     7801 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7861 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     7921 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7981 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8041 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8101 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8161 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8221 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccggcggcg
     8281 ggtccggagg agagggcaga ggaagtctcc taacatgcgg tgacgtggag gagaatcctg
     8341 gcccaatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     8401 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     8461 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     8521 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     8581 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaggacg
     8641 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     8701 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     8761 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     8821 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     8881 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     8941 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     9001 ccagatactg cgacctccct agcaaactgg ggcacaagct taattgagcg gccgctaggt
     9061 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9121 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     9181 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     9241 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     9301 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     9361 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     9421 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     9481 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     9541 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     9601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     9781 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     9841 attgctagcg ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9901 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9961 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    10021 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    10081 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10141 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10201 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10261 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10801 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
    10861 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10921 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10981 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11041 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11101 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11161 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11221 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11281 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11341 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11401 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11461 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11521 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11581 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11641 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11701 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11761 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11821 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11881 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    12001 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    12061 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    12121 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    12181 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    12241 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
    12301 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    12361 tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
