LOCUS       sequence-192980-        7420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AimR gene from the B. subtilis phage phi3T, with its 
            native promoter, C' His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, 
            Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek 
            R
  TITLE     Communication between viruses guides lysis-lysogeny decisions.
  JOURNAL   Nature. 2017 Jan 26;541(7638):488-493. doi: 10.1038/nature21049. 
            Epub 2017 Jan 18.
  PUBMED    28099413
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21049"; journalName: "Nature"; date: "2017-01-26- 
            26"; volume: "541"; issue: "7638"; pages: "488-493"
COMMENT     Sequence Label: pAimR
FEATURES             Location/Qualifiers
     source          1..7420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..40
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1194..1213)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      complement(3694..4282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3774..3793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4453..5244,5245..5313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5076..5095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5314..5418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5486..5504
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attattaaac cttatctcta ttcaccacca ccaccaccac taactgcagt ccggcaaaaa
       61 agggcaaggt gtcaattctc atgtttgaca gcttatcatc ggcaatagtt acccttatta
      121 tcaagataag aaagaaaagg atttttcgct acgctcaaat cctttaaaaa aacacaaaag
      181 accacatttt ttaatgtggt cttttattct tcaactaaag cacccattag ttcaacaaac
      241 gaaaattgga taaagtggga tatttttaaa atatatattt atgttacagt aatattgact
      301 tttaaaaaag gattgattct aatgaagaaa gcagacaagt aagcctccta aattcacttt
      361 agataaaaat ttaggaggca tatcaaatga actttaataa aattgattta gacaattgga
      421 agagaaaaga gatatttaat cattatttga accaacaaac gacttttagt ataaccacag
      481 aaattgatat tagtgtttta taccgaaaca taaaacaaga aggatataaa ttttaccctg
      541 catttatttt cttagtgaca agggtgataa actcaaatac agcttttaga actggttaca
      601 atagcgacgg agagttaggt tattgggata agttagagcc actttataca atttttgatg
      661 gtgtatctaa aacattctct ggtatttgga ctcctgtaaa gaatgacttc aaagagtttt
      721 atgatttata cctttctgat gtagagaaat ataatggttc ggggaaattg tttcccaaaa
      781 cacctatacc tgaaaatgct ttttctcttt ctattattcc atggacttca tttactgggt
      841 ttaacttaaa tatcaataat aatagtaatt accttctacc cattattaca gcaggaaaat
      901 tcattaataa aggtaattca atatatttac cgctatcttt acaggtacat cattctgttt
      961 gtgatggtta tcatgcagga ttgtttatga actctattca ggaattgtca gataggccta
     1021 atgactggct tttataatat gagataatgc cgactgtact ttttacagtc ggttttctaa
     1081 tgtcactaac ctgccccgtt agttgaagaa ggtttttata ttacagctcc agatcctcta
     1141 cgccggacgc atcgtggcag gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     1201 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     1261 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     1321 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     1381 aatgcaggag tcgcataagg gagagcgtcg acatggatga gcgatgatga tatccgttta
     1441 ggctgggcgg tgatagcttc tcgttcaggc agtacgcctc ttttcttttc cagacctgag
     1501 ggaggcggaa atggtgtgag gttcccgggg aaaagccaaa taggcgatcg cgggagtgct
     1561 ttatttgaag atcaggctat cactgcggtc aatagatttc acaatgtgat ggctggacag
     1621 cctgaggaac tctcgaaccc gaatggaaac aaccagatat ttatgaatca gcgcggctca
     1681 catggcgttg tgctggcaaa tgcaggttca tcctctgtct ctatcaatac ggcaacaaaa
     1741 ttgcctgatg gcaggtatga caataaagct ggagcgggtt catttcaagt gaacgatggt
     1801 aaactgacag gcacgatcaa tgccaggtct gtagctgtgc tttatcctga tgatattgca
     1861 aaagcgcctc atgttttcct tgagaattac aaaacaggtg taacacattc tttcaatgat
     1921 caactgacga ttaccttgcg tgcagatgcg aatacaacaa aagccgttta tcaaatcaat
     1981 aatggaccag agacggcgtt taaggatgga gatcaattca caatcggaaa aggagatcca
     2041 tttggcaaaa catacaccat catgttaaaa ggaacgaaca gtgatggtgt aacgaggacc
     2101 gagaaataca gttttgttaa aagagatcca gcgtcggcca aaaccatcgg ctatcaaaat
     2161 ccgaatcatt ggagccaggt aaatgcttat atctataaac atgatgggag ccgagtaatt
     2221 gaattgaccg gatcttggcc tggaaaacca atgactaaaa atgcagacgg aatttacacg
     2281 ctgacgctgc ctgcggacac ggatacaacc aacgcaaaag tgatttttaa taatggcagc
     2341 gcccaagtgc ccggtcagaa tcagcctggc tttgattacg tgctaaatgg tttatataat
     2401 gactcgggct taagcggttc tcttccccat tgagggcaag gctagacggg acttaccgaa
     2461 agaaaccatc aatgatggtt tcttttttgt tcataaatca gacaaaactt ttctcttgca
     2521 aaagtttgtg aagtgttgca caatataaat gtgaaatact tcacaaacaa aaagacatca
     2581 aagagaaaca taccctgcaa ggatgctgat attgtctgca tttgcgccgg agcaaaccaa
     2641 aaacctggtg agacacgcct tgaattagta gaaaagaact tgaagatttt caaaggcatc
     2701 gttagtgaag tcatggcgag cggatttgac ggcattttct tagtcgcgac gcgaggctgg
     2761 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     2821 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     2881 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     2941 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     3001 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct actgaagtgg atttctttaa
     3061 gagctccttt aacttcctca ccagtagttg tatcggtacc ataagtagaa gcagcaaccc
     3121 aagtagcttt accagcatcc ggttcaacca gcatagtaag aatcttactg gacatcggca
     3181 gttcttcgaa cagtgcgcca actaccagct ctttctccag aatgggctat acctctttta
     3241 cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg agagtgtgat
     3301 gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct
     3361 atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta cttttctgga
     3421 gttctttagc aaattttttt attagctgaa cttagtatta gtggccatac tcctccaatc
     3481 caaagctatt tagaaagatt actatatcct caaacaggcg gtaaccggcc tcttcatcgg
     3541 gaatgcgcgc gaccttcagc atcaccggca tgtccccctg gcgaccttca gcatcaccgg
     3601 catgtccccc tggcgcgacc ttcagcatca ccggcatgtc cccctggcgg acgggaagta
     3661 tccagctcga ggtcgggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4441 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4501 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4561 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4621 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4681 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4741 tagtttgcgc aacgttgttg ccattgctgc tggcatcgtg gtgtcacgct cgtcgtttgg
     4801 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4861 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4921 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4981 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5041 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     5101 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5161 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5221 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5281 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5401 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     5461 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     5521 attaacaaaa ttctccagtc ttcacatcgg tttgaaagga ggaagcggaa gaatgaagta
     5581 agagggattt ttgactccga agtaagtctt caaaaaatca aataaggagt gtcaagaatg
     5641 tttgcaaaac gattcaaaac ctctttactg ccgttattcg ctggattttt attgctgttt
     5701 catttggttc tggcaggacc ggcggctgcg agtgctgaaa cggcgaacaa atcgaatgag
     5761 cttacagcac cgtcgatcaa aagcggaacc attcttcatg catggaattg gtcgttcaat
     5821 acgttaaaac acaatatgaa ggatattcat gatgcaggat atacagccat tcagacatct
     5881 ccgattaacc aagtaaagga agggaatcaa ggagataaaa gcatgtcgaa ctggtactgg
     5941 ctgtatcagc cgacatcgta tcaaattggc aaccgttact taggtactga acaagaattt
     6001 aaagaaatgt gtgcagccgc tgaagaatat ggcataaagg tcattgttga cgcggtcatc
     6061 aatcatacca ccagtgatta tgccgcgatt tccaatgagg ttaagagtat tccaaactgg
     6121 acacatggaa acacacaaat taaaaactgg tctgatcgga tcctagaagc ttatcgaatt
     6181 cattgattta acaacaaata aagaataaaa ataaaatata tacttgaaat tctgacaact
     6241 atgaggtata ttataaacaa gggaagtgat aactgaataa aggggggaga caattgaaat
     6301 taaagcagat gattaagaat gaatgcgaaa aggataatca gcttgcagct cgacttgcaa
     6361 aattggccgg ttatgaaaaa gtaaatggtt tttataagtt tgtgaacacc ccagaaaaag
     6421 aaatggaaaa cttgggtgga ttactcaaga tcgtaaaaaa cttgtttcct gatagtgaag
     6481 agcaactttt aagtgaatac ttcttagaat tagaccctaa taaaaaatgt gcaaggcaat
     6541 cagttgagta ctcagatata aaccaatggg acacacttac tgataagatt atcattaact
     6601 tatgcaactc aaaaaattcc acaagtcaag agtggggaaa agtttacagc ttacatagaa
     6661 aattaaacaa aaacgaaatc agtttaaatg atgctattag ggaatcaggg aaatgtaaaa
     6721 taaaatccgc ggaaatgctc ttcttttcaa atgcaatgct gatgtatgcg tatttaaaca
     6781 ttggtgaatt tggattaatg aagagcactt caaaattgtt agaatttgat gatttacccg
     6841 aagggttcat taaagagtca ttcaaaagca gagtatctat gctcgaagcg aacataagct
     6901 taaatgaaaa tagcctactt gaagcgagac agcattctaa ccgcgcaatt gaaaattcta
     6961 acgtgaatcg tatttgtttt tttgcatatt taacaattgg caacacttta atttttgagg
     7021 attatgatga ggccaaaaag gcgtacatta aaggtcaaaa atatgctaaa aatccagtgc
     7081 accaagaaat gcttgatggt gcgttgtgct ttttgtcaaa catctggaaa aaagaaaatc
     7141 aatgggtgaa ttataactct gataacatta aatatttgca attaagagct ttttattaca
     7201 taaatcaagg taacattgag gaagccacgg aaattttaga tgaactgtca tcaagagatc
     7261 aagatgagaa tgaattagga ttctactatt actacaaagg attaatatct caggataaga
     7321 cagactatta taaatcaata agatatttca aaaaatcaga tgataaatat tttatacaat
     7381 tgccattact tcaactcgaa cgaatggggg ctgatcttga
//
