LOCUS       sequence-192986-        6017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Zhang H, Han GW, Batyuk A, Ishchenko A, White KL, Patel N, Sadybekov
            A, Zamlynny B, Rudd MT, Hollenstein K, Tolstikova A, White TA, 
            Hunter MS, Weierstall U, Liu W, Babaoglu K, Moore EL, Katz RD, 
            Shipman JM, Garcia-Calvo M, Sharma S, Sheth P, Soisson SM, Stevens 
            RC, Katritch V, Cherezov V
  TITLE     Structural basis for selectivity and diversity in angiotensin II 
            receptors.
  JOURNAL   Nature. 2017 Apr 20;544(7650):327-332. doi: 10.1038/nature22035. 
            Epub 2017 Apr 5.
  PUBMED    28379944
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22035"; journalName: "Nature"; date: "2017-04-20- 
            20"; volume: "544"; issue: "7650"; pages: "327-332"
COMMENT     Sequence Label: IMPT-6213
FEATURES             Location/Qualifiers
     source          1..6017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     763..780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  914..1138
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1205..1738)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1908..1913,1914..1930,1931..1936))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2288..2379
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2304..2321
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2470..2493
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2494..2523
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2524..2544
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3910..4044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3947..3966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4001..4020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4073..4238
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4422..4876
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4509..4528)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4718..4739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4902..5006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5007..5075,5076..5867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5225..5244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      721 gagtgagctg ataccgctcg ccgcggccga acgaccgagc gcagcgagtc agtgagcgag
      781 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      841 cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatgcc
      901 ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact gaacaaaata gatctaaact
      961 atgacaataa agtcttaaac tagacagaat agttgtaaac tgaaatcagt ccagttatgc
     1021 tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt ttctgaagtg
     1081 caaattgccc gtcgtattaa agaggggcgt ggccaagggc atggtaaaga ctatattcgc
     1141 ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga
     1201 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
     1261 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
     1321 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
     1381 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
     1441 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
     1501 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat
     1561 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
     1621 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
     1681 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatca
     1741 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
     1801 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     1861 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
     1921 aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
     1981 caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg
     2041 tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc
     2101 tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc
     2161 cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc
     2221 gtttgttcgc ccaggactct agctatagtt ctagtggttg gctacgtata ctccggaata
     2281 ttaatagatc atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac
     2341 tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt
     2401 cccaccatcg ggcgcggatc catgaagaca atcatcgctc tctcttacat cttctgcctc
     2461 gtgttcgctg actacaagga cgacgacgac aagcaccatc accatcacca ccaccatcac
     2521 cacgagaact tgtacttcca gggagctgac ctggaggata actgggaaac cctgaacgac
     2581 aacctcaagg ttatcgaaaa ggccgacaac gctgcccagg tgaaggatgc tctgactaag
     2641 atgagggctg ccgctctcga cgcccaaaag gctacacccc ctaagctgga ggacaagtcg
     2701 cccgattccc ctgaaatgaa ggacttcaga cacggtttcg atatcctggt cggccaaatc
     2761 gacgatgcct tgaagctggc taacgagggc aaggtgaagg aagcccaggc cgctgccgag
     2821 caactgaaga ccactaggaa cgcttacatc cagaagtacc tcggttccgg ctcttgctct
     2881 caaaagccta gcgacaagca cctggatgcc atcccaatcc tctactacat catcttcgtt
     2941 atcggattct tggtgaacat cgtggtcgtt actctgttct gctgtcagaa gggtccaaag
     3001 aaggtctcct ctatctacat cttcaacctc gccgttgctg acctgctctt gctggccaca
     3061 ctccctttgt gggctaccta ctactcttac cgttacgatt ggctgttcgg accagtgatg
     3121 tgcaaggtct tcggttcctt cctgacactc aacatgttcg cttctatctt cttcatcacc
     3181 tgtatgtcag tggaccgcta ccagtcggtt atctacccat tcctcagcca gcgtcgcaac
     3241 ccgtggcaag cctcatacat cgttcccttg gtgtggtgca tggcttgtct cagctcattg
     3301 ccgactttct acttcaggga cgtgagaaca atcgagtacc tgggcgtcaa cgcctgtatc
     3361 atggctttcc caccggaaaa gtacgcccag tggtctgccg gcatcgcttt gatgaagaac
     3421 atcctgggat tcatcatccc tctgatcttc atcgctacct gctacttcgg tatccgtaag
     3481 cacctcttga agaccaacag ctacggcaag aaccgtatca ctcgcgacca agtcctgaag
     3541 atggctgccg ctgtggtcct cgccttcatc atctgctggt tgcccttcca cgtcctgacc
     3601 ttcctcgacg ccttggcttg gatgggcgtg atcaactcgt gtgaggtcat cgctgttatc
     3661 gatttggccc tgccgttcgc tatcctgctc ggattcacta actcatgcgt gaaccccttc
     3721 ctctactgtt tcgttggtaa ccgtttccag cagaagttga ggagcgtttt ccgtgtctaa
     3781 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
     3841 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3901 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3961 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4021 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc cgaaccagat
     4081 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
     4141 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
     4201 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
     4261 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
     4321 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
     4381 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     4441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4621 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4681 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
     4741 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4801 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4861 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4921 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5161 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5221 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5281 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5341 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5401 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5461 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5521 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5581 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5641 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5701 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5761 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5821 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5881 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5941 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6001 gagcgtcaga ccccgta
//
