LOCUS       sequence-19299-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf222
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             469..510
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             520..537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(560..577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    566..790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc agctgaccac catggacgga actcttttcc
       61 ccggagatga cgatcttgca attccagcaa ctgaattttt ttctacaaag gctgctaaaa
      121 agccagaggc taaacgcgaa gcaattgtta aagccgatga agacgacaat gaggaaactc
      181 tcaaacaacg gctaactaat ttggaaaaaa agattactaa tgtaacaaca aagtttgaac
      241 aaatagaaaa gtgttgtaaa cgcaacgatg aagttctatt taggttggaa aatcacgctg
      301 aaactctaag agcggctatg atatctctgg ctaaaaagat tgatgttcag actggacggc
      361 gcccatatga gtaagcaatt cccggggatc cgaataaagg gtcaagacaa ttctgcagat
      421 atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
      481 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
      541 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
      721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
      781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
      841 ccctgtagcg gcgccattag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      901 cttgccag
//
