LOCUS       sequence-19300-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf223
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             578..619
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(669..686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    675..786
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatgaactc tctatcaatt
       61 ttttttattg tggtagcgac ggctgcggtg tgtttacttt ttatccaggg ttactcaata
      121 tatgaaaatt atggcaatat taaggaattt aatgctactc atgcagcatt cgaatattca
      181 aaatctatag gtggaacacc ggcattagat aggagagttc aagatgtcaa cgacacaatt
      241 tctgatgtaa agcaaaagtg gagatgtgtg gtttatccag gaaacggttt tgtatccgct
      301 tccatatttg gatttcaggc agaagttgga cccaataata ctagatccat tagaaaattt
      361 aacacgatgc aacaatgtat agactttaca ttttctgatg ttattaacat cgatatttat
      421 aatccatgtg ttgtaccaaa tataaataac gcagagtgtc agtttctaaa atctgtactt
      481 taagcaattc ccggggatcc gaataaaggg tcaagacaat tctgcagata tccagcacag
      541 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
      601 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
      661 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      721 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      781 tgcatcgcat tgtc
//
