LOCUS       sequence-193002-       11332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter to monitor translation of EMCV IRES mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Han K, Jaimovich A, Dey G, Ruggero D, Meyuhas O, Sonenberg N, Meyer 
            T
  TITLE     Parallel measurement of dynamic changes in translation rates in 
            single cells.
  JOURNAL   Nat Methods. 2014 Jan;11(1):86-93. doi: 10.1038/nmeth.2729. Epub 
            2013 Nov 10.
  PUBMED    24213167
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2729"; journalName: "Nat Methods"; date: "2014-01"; 
            volume: "11"; issue: "1"; pages: "86-93"
COMMENT     Sequence Label: IRES-H2B-YFP-DD
FEATURES             Location/Qualifiers
     source          1..11332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1725..1749
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        1770..2149
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2151..2426
                     /label=chicken beta-actin promoter
     intron          2427..3435
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3359..3381
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3443..3462
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    join(3516..4090,4091..4093,4094..4102)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3683..3700)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3910..3929
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4115..4492
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(4499..4501,4502..5212)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4541..4562)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4802..4821)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5149..5170
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5219..5695
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     protein_bind    complement(5710..5734)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5765..6353
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5818..5838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6236..6247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6367..6866
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6402..6425)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6799..6818
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6887..7486
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6887..6906)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7383..7403
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7624..7857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7929..8050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7966..7985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8020..8039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8090..8225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8245..8264)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8246..8264)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8274..8291)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8274..8290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8283..8305)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8432..8887
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8519..8538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8729..8750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8913..9017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9018..9086,9087..9878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9236..9255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10049..10637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10538..10557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10791..10808
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10925..10946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10961..10966,10967..10984,10985..10991)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10999..11015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11004..11026
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11023..11039
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11023..11039
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11058..11078
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11060..11078
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11106..11332
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
     1741 aagcaggctt taaaggaacc aattcagtcg acattgatta ttgactagtt attaatagta
     1801 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     1861 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     1921 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     1981 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2041 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2101 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     2161 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     2221 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg cgcgcgccag
     2281 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     2341 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     2401 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc cgtgccccgc
     2461 tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     2521 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggctcg
     2581 tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct ttgtgcgggg
     2641 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggcccg
     2701 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcgtgt
     2761 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg cgaggggaac
     2821 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc
     2881 gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     2941 tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     3001 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     3061 cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     3121 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg gagccgaaat
     3181 ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg gcgccggcag
     3241 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccatct
     3301 ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg cagggcgggg
     3361 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     3421 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     3481 aaagaattgc tcgagaccgg cgcgcctaaa ttccgcccct ctccctcccc cccccctaac
     3541 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3601 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3661 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3721 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3781 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3841 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3901 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3961 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4021 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4081 cgatgataat atggccacaa ccatggctag gaccatgcca gagccagcga agtctgctcc
     4141 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
     4201 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     4261 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     4321 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     4381 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     4441 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatccagt
     4501 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4561 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4621 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4681 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     4741 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4801 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     4861 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4921 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     4981 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     5041 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5101 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5161 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca aggtcgagat
     5221 cagtctgatt gcggcgttag cggtagatca cgttatcggc atggaaaccg tcatgccgtg
     5281 gaacctgcct gccgatctcg cctggtttaa acgcaacacc ttaaataaac ccgtgattat
     5341 gggccgccat acctgggaat caatcggtcg tccgttgcca ggacgcaaaa atattatcct
     5401 cagcagtcaa ccgagtacgg acgatcgcgt aacgtgggtg aagtcggtgg atgaagccat
     5461 cgcggcgtgt ggtgacgtac cagaaatcat ggttattggc ggcggtcgcg tttatgaaca
     5521 gttcttgcca aaagcgcaaa aactgtatct gacgcatatc gacgcagaag tggaaggcga
     5581 cacccatttc ccggattacg agccggatga ctgggaatcg gtattcagcg aattccacga
     5641 tgctgatgcg cagaactctc acagctattg ctttgagatt ctggagcggc gatgagtcga
     5701 gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     5761 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5821 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5881 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5941 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6001 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6061 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6121 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6181 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6241 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6301 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     6361 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     6421 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     6481 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     6541 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     6601 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     6661 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     6721 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     6781 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     6841 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     6901 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6961 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7021 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7081 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7141 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7201 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7261 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7321 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7381 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7441 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     7501 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     7561 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7621 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     7681 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7741 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7801 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7861 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7921 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7981 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8041 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     8101 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8161 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8221 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8281 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8341 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8401 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8461 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8521 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8581 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8641 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8701 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8761 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8821 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8881 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8941 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9001 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9061 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9121 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9181 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9241 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9301 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9361 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9421 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9481 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9541 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9601 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9661 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9721 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9781 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9841 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9901 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9961 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10021 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10081 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10141 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10201 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10261 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10321 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10381 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10441 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10501 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10561 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10621 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10681 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10741 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10801 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10861 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10921 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10981 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    11041 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    11101 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
    11161 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
    11221 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
    11281 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa ac
//
