LOCUS       sequence-193004-        7521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ 
            (1079-1751).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Park S, Stanfield RL, Martinez-Yamout MA, Dyson HJ, Wilson IA, 
            Wright PE
  TITLE     Role of the CBP catalytic core in intramolecular SUMOylation and 
            control of histone H3 acetylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114.
  PUBMED    28630323
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114."
COMMENT     Sequence Label: His6tev CBP BCHZ (1079-1751)
FEATURES             Location/Qualifiers
     source          1..7521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     508..527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     761..778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    793..932
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(858..877)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1018..1040
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1034..1225)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2034..3116)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3078..3097
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3117..3194)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3400..3419
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3503..3522
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3503..3521
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3522..3546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3561..3583
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3603..3620
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3642..3662
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      5310..5319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5755..5772
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5825..5843)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5839..5886
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5923..6378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6010..6029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6220..6241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6404..6508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6509..6577,6578..7369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6727..6746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
       61 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      121 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      781 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      841 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
      901 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
      961 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     1021 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     1081 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     1141 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     1201 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     1261 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     1321 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     1381 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     1441 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     1501 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     1561 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     1621 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     1681 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     1741 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     1801 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     1861 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     1921 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     1981 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     2041 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2101 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     2161 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     2221 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     2281 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     2341 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     2401 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     2461 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     2521 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     2581 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     2641 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     2701 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     2761 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     2821 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     2881 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     2941 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     3001 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     3061 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     3121 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
     3181 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca
     3241 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
     3301 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     3361 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     3421 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     3481 cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
     3541 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atgtcgtact
     3601 accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg tattttcagg
     3661 gcgccatggg atcctcccag ccacgcaaaa aaatctttaa acccgaggag ctacgccagg
     3721 cacttatgcc aactctagaa gcactctatc gacaggaccc agagtctttg ccttttcgtc
     3781 agcctgtaga tcctcagctc ctaggaatcc cagattattt tgatatagtg aagaatccta
     3841 tggacctttc taccatcaaa cgaaagctgg acacagggca atatcaagaa ccctggcagt
     3901 atgtggatga tgtctggctt atgttcaaca atgcgtggct atataatcgt aaaacgtccc
     3961 gtgtatataa attttgcagt aaacttgcag aggtctttga acaagaaatt gaccctgtca
     4021 tgcagtctct tggatattgc tgtggacgaa agtatgagtt ctccccacag actttgtgct
     4081 gttacggaaa gcagctgtgt acaattcctc gtgatgcagc ctactacagc tatcagaata
     4141 ggtatcattt ctgtgagaag tgtttcacag agatccaggg cgagaatgtg accctgggtg
     4201 acgacccttc ccaacctcag acgacaattt ccaaggatca atttgaaaag aagaaaaatg
     4261 ataccttaga tcctgaacct tttgttgact gcaaagagtg tggccggaag atgcatcaga
     4321 tttgtgttct acactatgac atcatttggc cttcaggttt tgtgtgtgac aactgtttga
     4381 agaaaactgg cagacctcgg aaagaaaaca aattcagtgc taagaggctg cagaccacac
     4441 gattgggaaa ccacttagaa gacagagtga ataagttttt gcggcgccag aatcaccctg
     4501 aagctgggga ggtttttgtc agagtggtgg ccagctcaga caagactgtg gaggtcaagc
     4561 cgggaatgaa gtcaaggttt gtggattctg gagagatgtc ggaatctttc ccatatcgta
     4621 ccaaagcact ctttgctttt gaggagatcg atggagtcga tgtgtgcttt tttgggatgc
     4681 atgtgcaaga atacggctct gattgccccc caccaaatac aaggcgtgta tacatatctt
     4741 atctggacag tattcatttc ttccggcccc gctgcctccg gacagctgtt taccatgaga
     4801 tcctcatcgg atatctcgag tatgtgaaga aattggggta tgtgacagga catatttggg
     4861 cctgtccccc aagtgaagga gatgactata tctttcattg ccacccccct gaccagaaaa
     4921 tccccaaacc aaaacgacta caggagtggt acaagaagat gctggacaag gcgtttgcag
     4981 agaggatcat taacgactat aaggacatct tcaaacaagc gaacgaagac aggctcacga
     5041 gtgccaagga gttgccctat tttgaaggag atttctggcc taatgtgttg gaagaaagca
     5101 ttaaggaact agaacaagaa gaagaagaaa ggaaaaaaga agagagtact gcagcgagtg
     5161 agactcctga gggcagtcag ggtgacagca aaaatgcgaa gaaaaagaac aacaagaaga
     5221 ccaacaaaaa caaaagcagc attagccgcg ccaacaagaa gaagcccagc atgcccaatg
     5281 tttccaacga cctgtcgcag aagctgtatg ccaccatgga gaagcacaag gaggtattct
     5341 ttgtgattca tctgcatgct gggcctgtta tcagcactca gccccccatc gtggaccctg
     5401 atcctctgct tagctgtgac ctcatggatg ggcgagatgc cttcctcacc ctggccagag
     5461 acaagcactg ggaattctct tccttacgcc gctccaaatg gtccactctg tgcatgctgg
     5521 tggagctgca cacacagggc caggaccgct ttgtttatac ctgcaatgag tgcaaacacc
     5581 atgtggaaac acgctggcac tgcactgtgt gtgaggacta tgacctttgt atcaattgct
     5641 acaacacaaa gagccacacc cataagatgg tgaagtgggg gctaggccta gattaatagg
     5701 tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct cgagcaccac
     5761 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     5821 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     5881 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
     5941 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6001 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6061 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6121 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6181 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6241 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6301 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6361 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6721 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7021 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     7081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7381 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7441 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7501 ctgagcgtca gaccccgtag a
//
