LOCUS       sequence-193024-       10068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor scafold with dTOMATO positive selection module, and tagBFP 
            negative selection module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-tagBFP-PSM-dTOMATO
FEATURES             Location/Qualifiers
     source          1..10068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        75..378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..659
                     /label=chicken beta-actin promoter
     intron          660..1668
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1727..2428
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    2572..2627
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   2990..3024
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        3370..3673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3676..3954
                     /label=chicken beta-actin promoter
     intron          3955..4963
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     regulatory      5016..5025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5022..5723
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5155..5173)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5423..5442
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5617..5640
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             5724..5777
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5778..6374
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6271..6291
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6518..6573
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   7103..7165
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7216..7236)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7216..7234)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7255..7271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7255..7271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7268..7290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7279..7295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7303..7309,7310..7327,7328..7333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7348..7369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      7606..8194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8095..8114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8348..8365
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(join(8416..9207,9208..9276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9039..9058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9277..9381)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9407..9862)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9544..9565)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9756..9775
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9989..10011
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10003..10020
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10004..10020
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10030..10049
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10030..10048
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
       61 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      361 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
      421 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
      481 gggggcgggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc
      721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      781 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      841 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg
      961 ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg
     1021 tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1081 ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc
     1141 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc
     1201 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1261 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt
     1321 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1381 cttcctttgt cccaaatctg gcggagccga aatctgggag gcgccgccgc accccctcta
     1441 gcgggcgcgg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     1501 tgcgtcgccg cgccgccgtc cccttctcca tctccagcct cggggctgcc gcagggggac
     1561 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1621 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1681 gtgctggtta ttgtgctgtc tcatcatttt ggcaaaggcc gccaccatga gcgagctgat
     1741 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     1801 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     1861 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     1921 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     1981 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     2041 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     2101 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     2161 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     2221 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     2281 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     2341 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     2401 tagcaaactg gggcacaagc ttaattaaca ctcctcaggt gcaggctgcc tatcagaagg
     2461 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     2521 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     2581 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     2641 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     2701 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
     2761 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2821 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     2881 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     2941 ttatggagat ccctcgacct gcagcccaag cttggatccc ctaggttaac cctagaaaga
     3001 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     3061 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     3121 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     3181 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     3241 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa
     3301 ctagaatgct agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3361 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3421 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3661 cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     3721 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     3781 cggggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     3841 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     3901 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     3961 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     4021 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     4081 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     4141 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     4201 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     4261 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     4321 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     4381 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     4441 gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc
     4501 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     4561 gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg gctgtcgagg
     4621 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     4681 tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     4741 cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     4801 cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcggg gggacggctg
     4861 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     4921 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     4981 ggttattgtg ctgtctcatc attttggcaa aggccgccac catggtgagc aagggcgagg
     5041 aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
     5101 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     5161 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     5221 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     5281 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     5341 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     5401 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     5461 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     5521 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
     5581 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     5641 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     5701 acggcatgga cgagctgtac aaggagggca gaggaagtct tctaacatgc ggtgacgtgg
     5761 aggagaatcc cggccctacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     5821 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     5881 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     5941 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     6001 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     6061 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     6121 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     6181 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     6241 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     6301 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     6361 agcccggtgc ctgacactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     6421 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     6481 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     6541 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     6601 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     6661 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     6721 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     6781 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     6841 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     6901 ctcgacctgc agcccaagct tggatccctc gacttttgtt actttataga agaaattttg
     6961 agtttttgtt tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta
     7021 ttagtatgta agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct
     7081 cgatatacag accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg
     7141 tcacaatatg attatctttc tagggttaac ctagtatacg cgtatgcggc cgcttaatta
     7201 atccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     7261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7441 acgcgcgggg agaggcggtt tgcgtattgg gcgcttaaaa cttcattttt aatttaaaag
     7501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7741 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8221 gttcctgggc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8281 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8341 cgagcgcagc gagtcagtga gcgaggaagc ggaagaatca atctaaagta tatatgagta
     8401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     9421 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     9481 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9541 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9601 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9661 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9721 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9781 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9841 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9901 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     9961 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    10021 gagcgcgcgt aatacgactc actatagggc gaattggggc gcgccatt
//
