LOCUS       sequence-19304-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf227
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             340..381
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             391..408
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(431..448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    437..661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tacccttcac cgaatttcag ctgaaccacc atggggaaaa tgttagaata tttttgtcat
       61 tgatgtcatt atctatagag ataacacatc ctccgacaga actaatacag agtataatag
      121 tcgatagtct atggggaata agatggtaca tctcattgtc atttacaact tctacgctta
      181 agatgttggt aagaagtacc gtttcatcca tctgtctatt gatctcatca gatataattt
      241 tttgagcaat tcccggggat ccgaataaag ggtcaagaca attctgcaga tatccagcac
      301 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
      361 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
      421 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      481 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      541 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      601 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      661 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
      721 ggcgcattaa gcgcggcggg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      781 cg
//
