LOCUS       sequence-193047-        8945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric KLF4-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN32
FEATURES             Location/Qualifiers
     source          1..8945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             526..1233
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(671..689)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     939..958
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1243..1299
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2812..3400
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2865..2885)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3283..3294)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3553..3733
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3805..3926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3842..3861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3896..3915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3966..4101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4121..4140)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4122..4140)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4150..4167)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4150..4166)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4159..4181)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4308..4763
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4395..4414)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4605..4626
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4789..4893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4894..4962,4963..5754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5112..5131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5925..6513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6414..6433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6667..6684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6801..6822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6837..6842,6843..6860,6861..6867)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6875..6891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6880..6902
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6899..6915
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6899..6915
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6934..6954
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6936..6954
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6982..7208
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7209..7389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7436..7561
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8054..8287
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8472..8516
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8783..8900
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      601 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      661 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      721 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      781 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      841 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      901 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      961 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1021 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1081 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1141 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1201 cactccaccg gcggcatgga cgagctgtac aagggatctg gagcaacaaa cttctcacta
     1261 ctcaaacaag caggtgacgt ggaggagaat cccggcccta tggctgtcag cgacgcgctg
     1321 ctcccatctt tctccacgtt cgcgtctggc ccggcgggaa gggagaagac actgcgtcaa
     1381 gcaggtgccc cgaataaccg ctggcgggag gagctctccc acatgaagcg acttccccca
     1441 gtgcttcccg gccgccccta tgacctggcg gcggcgaccg tggccacaga cctggagagc
     1501 ggcggagccg gtgcggcttg cggcggtagc aacctggcgc ccctacctcg gagagagacc
     1561 gaggagttca acgatctcct ggacctggac tttattctct ccaattcgct gacccatcct
     1621 ccggagtcag tggccgccac cgtgtcctcg tcagcgtcag cctcctcttc gtcgtcgccg
     1681 tcgagcagcg gccctgccag cgcgccctcc acctgcagct tcacctatcc gatccgggcc
     1741 gggaacgacc cgggcgtggc gccgggcggc acgggcggag gcctcctcta tggcagggag
     1801 tccgctcccc ctccgacggc tcccttcaac ctggcggaca tcaacgacgt gagcccctcg
     1861 ggcggcttcg tggccgagct cctgcggcca gaattggacc cggtgtacat tccgccgcag
     1921 cagccgcagc cgccaggtgg cgggctgatg ggcaagttcg tgctgaaggc gtcgctgagc
     1981 gcccctggca gcgagtacgg cagcccgtcg gtcatcagcg tcagcaaagg cagccctgac
     2041 ggcagccacc cggtggtggt ggcgccctac aacggcgggc cgccgcgcac gtgccccaag
     2101 atcaagcagg aggcggtctc ttcgtgcacc cacttgggcg ctggaccccc tctcagcaat
     2161 ggccaccggc cggctgcaca cgacttcccc ctggggcggc agctccccag caggactacc
     2221 ccgaccctgg gtcttgagga agtgctgagc agcagggact gtcaccctgc cctgccgctt
     2281 cctcccggct tccatcccca cccggggccc aattacccat ccttcctgcc cgatcagatg
     2341 cagccgcaag tcccgccgct ccattaccaa gagctcatgc cacccggttc ctgcatgcca
     2401 gaggagccca agccaaagag gggaagacga tcgtggcccc ggaaaaggac cgccacccac
     2461 acttgtgatt acgcgggctg cggcaaaacc tacacaaaga gttcccatct caaggcacac
     2521 ctgcgaaccc acacaggtga gaaaccttac cactgtgact gggacggctg tggatggaaa
     2581 ttcgcccgct cagatgaact gaccaggcac taccgtaaac acacggggca ccgcccgttc
     2641 cagtgccaaa aatgcgaccg agcattttcc aggtcggacc acctcgcctt acacatgaag
     2701 aggcattttc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     2761 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgtgagtcga caatcaacct
     2821 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2881 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2941 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3001 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3061 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3121 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3181 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     3241 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3301 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3361 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     3421 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3481 ggggactgga agggctaatt cactcccaac gaagacaaga tatcctgcta gctccggatc
     3541 cggacctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3601 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3661 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3721 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     3781 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     3841 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3901 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     3961 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4021 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4081 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     4141 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4201 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4261 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     4321 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4381 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4441 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4501 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4561 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4621 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4681 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4741 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     4801 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4861 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4921 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4981 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5041 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5101 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5161 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5221 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5281 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5341 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5401 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5461 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5521 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5581 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5641 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5701 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5761 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5821 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5881 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5941 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6001 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6061 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6121 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6181 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6241 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6301 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6361 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6421 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6481 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6541 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6601 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6661 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6721 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6781 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggtttta
     6841 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6901 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     6961 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     7021 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     7081 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     7141 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     7201 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7261 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7321 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7381 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     7441 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     7501 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     7561 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     7621 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7681 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7741 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7801 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7861 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7921 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7981 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     8041 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     8101 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     8161 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     8221 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     8281 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     8341 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     8401 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     8461 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     8521 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     8581 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     8641 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     8701 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     8761 gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag
     8821 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     8881 ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagaagctt gatatcgaat
     8941 tccac
//
