LOCUS       sequence-193057-        9050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric KLF4-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN31
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             526..1233
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(671..689)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     939..958
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1243..1299
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2917..3505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2970..2990)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3388..3399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3658..3838
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3910..4031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3947..3966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4001..4020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4071..4206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4226..4245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4227..4245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4255..4272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4255..4271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4264..4286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4413..4868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4500..4519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4710..4731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4894..4998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4999..5067,5068..5859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5217..5236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6030..6618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6519..6538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6772..6789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6906..6927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6942..6947,6948..6965,6966..6972)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6980..6996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6985..7007
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7004..7020
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7004..7020
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7039..7059
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7041..7059
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7087..7313
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7314..7494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7541..7666
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8159..8392
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8577..8621
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8888..9005
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      601 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      661 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      721 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      781 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      841 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      901 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      961 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1021 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1081 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1141 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1201 cactccaccg gcggcatgga cgagctgtac aagggatctg gagcaacaaa cttctcacta
     1261 ctcaaacaag caggtgacgt ggaggagaat cccggcccta tggctgtcag cgacgcgctg
     1321 ctcccatctt tctccacgtt cgcgtctggc ccggcgggaa gggagaagac actgcgtcaa
     1381 gcaggtgccc cgaataaccg ctggcgggag gagctctccc acatgaagcg acttccccca
     1441 gtgcttcccg gccgccccta tgacctggcg gcggcgaccg tggccacaga cctggagagc
     1501 ggcggagccg gtgcggcttg cggcggtagc aacctggcgc ccctacctcg gagagagacc
     1561 gaggagttca acgatctcct ggacctggac tttattctct ccaattcgct gacccatcct
     1621 ccggagtcag tggccgccac cgtgtcctcg tcagcgtcag cctcctcttc gtcgtcgccg
     1681 tcgagcagcg gccctgccag cgcgccctcc acctgcagct tcacctatcc gatccgggcc
     1741 gggaacgacc cgggcgtggc gccgggcggc acgggcggag gcctcctcta tggcagggag
     1801 tccgctcccc ctccgacggc tcccttcaac ctggcggaca tcaacgacgt gagcccctcg
     1861 ggcggcttcg tggccgagct cctgcggcca gaattggacc cggtgtacat tccgccgcag
     1921 cagccgcagc cgccaggtgg cgggctgatg ggcaagttcg tgctgaaggc gtcgctgagc
     1981 gcccctggca gcgagtacgg cagcccgtcg gtcatcagcg tcagcaaagg cagccctgac
     2041 ggcagccacc cggtggtggt ggcgccctac aacggcgggc cgccgcgcac gtgccccaag
     2101 atcaagcagg aggcggtctc ttcgtgcacc cacttgggcg ctggaccccc tctcagcaat
     2161 ggccaccggc cggctgcaca cgacttcccc ctggggcggc agctccccag caggactacc
     2221 ccgaccctgg gtcttgagga agtgctgagc agcagggact gtcaccctgc cctgccgctt
     2281 cctcccggct tccatcccca cccggggccc aattacccat ccttcctgcc cgatcagatg
     2341 cagccgcaag tcccgccgct ccattaccaa gagctcatgc cacccggttc ctgcatgcca
     2401 gaggagccca agccaaagag gggaagacga tcgtggcccc ggaaaaggac cgccacccac
     2461 acttgtgatt acgcgggctg cggcaaaacc tacacaaaga gttcccatct caaggcacac
     2521 ctgcgaaccc acacaggtga gaaaccttac cactgtgact gggacggctg tggatggaaa
     2581 ttcgcccgct cagatgaact gaccaggcac taccgtaaac acacggggca ccgcccgttc
     2641 cagtgccaaa aatgcgaccg agcattttcc aggtcggacc acctcgcctt acacatgaag
     2701 aggcattttc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     2761 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     2821 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     2881 gcccttggaa ttgacgagta cggtgggtga gtcgacaatc aacctctgga ttacaaaatt
     2941 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3001 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3061 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3121 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3181 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3241 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3301 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3361 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3421 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3481 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     3541 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3601 taattcactc ccaacgaaga caagatatcc tgctagctcc ggatccggac ctgtactggg
     3661 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3721 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3781 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3841 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     3901 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3961 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4021 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4141 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4201 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4261 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4321 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4381 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4861 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4921 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4981 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5041 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5101 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5161 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5221 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5281 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5341 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5401 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5461 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5521 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5581 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5641 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5701 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5761 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5821 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5881 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5941 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6001 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6061 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6781 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6841 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6901 gcaattaatg tgagttagct cactcattag gcaccccagg ttttacactt tatgcttccg
     6961 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7021 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7081 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7141 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7201 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7261 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7501 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7561 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7621 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7681 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7741 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7801 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     7861 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     7921 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     7981 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8041 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8101 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8161 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8221 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8281 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8341 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8401 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8461 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8521 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8581 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8641 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8701 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8761 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     8821 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     8881 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     8941 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9001 attttatcga tcacgagact agcctcgaga agcttgatat cgaattccac
//
