LOCUS       sequence-193058-        8525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric SOX2-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN22
FEATURES             Location/Qualifiers
     source          1..8525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(526..528,529..531,532..1242)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2266..2376
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     misc_feature    2392..2980
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2445..2465)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2863..2874)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3133..3313
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3385..3506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3422..3441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3476..3495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3546..3681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3701..3720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3702..3720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3730..3747)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3730..3746)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3739..3761)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3888..4343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3975..3994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4185..4206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4369..4473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4474..4542,4543..5334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4692..4711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5505..6093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5994..6013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6247..6264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6381..6402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6417..6422,6423..6440,6441..6447)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6455..6471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6460..6482
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6479..6495
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6479..6495
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6514..6534
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6516..6534
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6562..6788
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6789..6969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7016..7141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7634..7867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8052..8096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8363..8480
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      721 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactct cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat gtacaacatg
     1321 atggagacgg agctgaagcc gccgggcccg cagcaaactt cggggggcgg cggcggcaac
     1381 tccaccgcgg cggcggccgg cggcaaccag aaaaacagcc cggaccgcgt caagcggccc
     1441 atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca agatggccca ggagaacccc
     1501 aagatgcaca actcggagat cagcaagcgc ctgggcgccg agtggaaact tttgtcggag
     1561 acggagaagc ggccgttcat cgacgaggct aagcggctgc gagcgctgca catgaaggag
     1621 cacccggatt ataaataccg gccccggcgg aaaaccaaga cgctcatgaa gaaggataag
     1681 tacacgctgc ccggcgggct gctggccccc ggcggcaata gcatggcgag cggggtcggg
     1741 gtgggcgccg gcctgggcgc gggcgtgaac cagcgcatgg acagttacgc gcacatgaac
     1801 ggctggagca acggcagcta cagcatgatg caggaccagc tgggctaccc gcagcacccg
     1861 ggcctcaatg cgcacggcgc agcgcagatg cagcccatgc accgctacga cgtgagcgcc
     1921 ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc cacctacagc
     1981 atgtcctact cgcagcaggg cacccctggc atggctcttg gctccatggg ttcggtggtc
     2041 aagtccgagg ccagctccag cccccctgtg gttacctctt cctcccactc cagggcgccc
     2101 tgccaggccg gggacctccg ggacatgatc agcatgtatc tccccggcgc cgaggtgccg
     2161 gaacccgccg cccccagcag acttcacatg tcccagcact accagagcgg cccggtgccc
     2221 ggcacggcca ttaacggcac actgcccctc tcacacatgc cggccgacgc ccttgacgat
     2281 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     2341 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaagtcga caatcaacct
     2401 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2461 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2521 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2581 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2641 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2701 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2761 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     2821 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2881 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2941 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     3001 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3061 ggggactgga agggctaatt cactcccaac gaagacaaga tatcctgcta gctccggatc
     3121 cggacctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3181 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3241 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3301 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     3361 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     3421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3481 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     3541 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3601 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3661 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     3721 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3781 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3841 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     3901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3961 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4081 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4201 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4261 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4321 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     4381 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4441 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4501 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4561 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4621 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4681 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4741 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4801 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4861 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4921 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4981 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5041 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5101 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5161 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5221 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5281 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5341 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5401 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5461 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5521 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5581 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5641 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5701 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5761 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5821 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5881 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5941 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6001 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6061 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6121 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6181 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6241 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6301 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6361 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggtttta
     6421 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6481 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     6541 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     6601 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     6661 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     6721 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     6781 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     7021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     7081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     7141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     7201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     7621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     7681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     7741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     7801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     7861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     7921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     7981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     8041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     8101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     8161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     8221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     8281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     8341 gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag
     8401 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     8461 ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagaagctt gatatcgaat
     8521 tccac
//
