LOCUS       sequence-193070-        8497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral tet-inducible expression of mito-targeted genetically 
            encoded Ca2+-indicators for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Mughal W, Martens M, Field J, Chapman D, Huang J, Rattan S, Hai Y, 
            Cheung KG, Kereliuk S, West AR, Cole LK, Hatch GM, Diehl-Jones W, 
            Keijzer R, Dolinsky VW, Dixon IM, Parmacek MS, Gordon JW
  TITLE     Myocardin regulates mitochondrial calcium homeostasis and prevents 
            permeability transition.
  JOURNAL   Cell Death Differ. 2018 Mar 6. pii: 10.1038/s41418-018-0073-z. doi: 
            10.1038/s41418-018-0073-z.
  PUBMED    29511336
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2018 Mar 6. pii: 10.1038/s41418-018-0073-z. doi: 
            10.1038/s41418-018-0073-z."
COMMENT     Sequence Label: pLenti-Mito-Car-GECO
FEATURES             Location/Qualifiers
     source          1..8497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..210
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     161..181
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    212..230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             285..359
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             381..455
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             504..560
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     1256..1275
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1299..1742
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     CDS             1771..1812
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1822..1839
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1862..1879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(1891..1911)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1896..2225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2076..2211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2235..2834
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2235..2254)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2731..2751
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2906..3139
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3211..3332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3248..3267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3302..3321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3372..3507
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3527..3546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3528..3546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3556..3573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3556..3572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3565..3587)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3714..4169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3801..3820)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4011..4032
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4195..4299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4300..4368,4369..5160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4518..4537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5331..5919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5820..5839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6073..6090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6207..6228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6243..6248,6249..6266,6267..6273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6281..6297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6286..6308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6305..6321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6305..6321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6340..6360
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6342..6360
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6388..6614
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6615..6795
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6842..6967
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7460..7693
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7878..7922
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
       61 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      121 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      181 gtacggtggg aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc
      241 agtgatagag atcgtcgact agtggatcca gtgtggtgga attcatgtct gttctgactc
      301 ctctgctgct ccggggtctc acaggttccg caagaagact ccccgtgcct agggccaaaa
      361 ttcattcact gggggacccc atgagcgtgc tcaccccact cctgctgcgg gggctgaccg
      421 gcagcgctag gcggctgcca gtccccaggg ccaagatcca cagtctcggc gatcccaagg
      481 atccaaccat ggtcgactct tcacgtcgta agtggaataa ggcaggtcac gcagttagag
      541 ctataggtcg gctgagctca cccgtggttt ccgagcggat gtaccccgag gacggcgccc
      601 tgaagagcga gatcaagaag gggctgaggc tgaaggacgg cggccactac gccgccgagg
      661 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca
      721 tcaagttgga catcgtgtcc cacaacgagg actacaccat cgtggaacag tgcgaacgcg
      781 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggaggt acaggcggga
      841 gtctggtgag caagggcgtg gaggataaca tggccatcgt caaggagttc atgcgcttca
      901 agacgcacat agagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      961 gccgccccta cgaggccttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc
     1021 ccttcgcctg ggacatcctg tcccctcaga ttatgtacgg ctccaaggcc tacattaagc
     1081 acccagccga catccccgac tacttcaagc tgtccttccc cgagggcttc aggtgggagc
     1141 gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa ccaggactcc tccctgcagg
     1201 acggcgtatt catctacaag gtgaagctgc gcggcaccaa cttccccccc gacggccccg
     1261 taatgcagaa gaagaccatg ggctgggagc ctacgcgtga ccaactgact gaagagcaga
     1321 tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg acgataacaa
     1381 ccaaggagct ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc
     1441 aggacatgat caatgaagta gatgccgacg gtgacggcac attcgacttc cctgagttcc
     1501 tgacgatgat ggcaagaaaa atgaatgaca cagacagtga agaggaaatt agagaagcgt
     1561 tccgcgtgtt tgataaggac ggcaatggct acatcggcgc agcagagctt cgccacgtga
     1621 tgacagacct tggagagaag ttaacagatg aggaggttga tgaaatgatc agggtagcag
     1681 acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg acagcgaagt
     1741 aactcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     1801 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ctgctgatca
     1861 gcctcgactg tgccttctag tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
     1921 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2041 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2101 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2161 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     2221 gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     2281 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     2341 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     2401 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     2461 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     2521 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     2581 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     2641 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     2701 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     2761 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     2821 agcccggtgc ctgaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     2881 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2941 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3001 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3061 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3121 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     3181 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3361 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3421 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3481 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3541 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3601 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3661 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3721 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3781 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3841 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3901 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3961 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4021 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4081 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4141 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4201 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4261 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4321 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4381 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4441 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4501 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4561 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4621 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4681 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4741 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4801 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4861 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4921 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4981 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5041 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5101 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5161 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5221 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5281 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5341 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5401 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5461 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5521 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5581 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5641 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5701 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5761 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5821 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5881 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5941 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6001 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6061 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6121 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6301 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6361 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6421 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6481 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6541 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6601 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6661 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6721 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6781 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6841 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6901 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6961 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7021 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7081 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7141 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7201 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7261 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7321 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7381 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7441 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7501 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7561 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7621 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7681 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7741 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7801 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7861 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7921 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7981 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8041 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8101 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8161 tgaacggatc tcgacggtat cgataagctt gggagttccg cttacataac ttacggtaaa
     8221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     8281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     8341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     8401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     8461 tacttggcag tacatctacg tattagtcat cgctatt
//
