LOCUS       sequence-193100-        6085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A modified version of the Promega pGL4.23 vector. Contains the MYC 
            promoter in place of the minimal promoter and polyadenylation 
            signals flanking the enhancer cloning site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: pGL4.23-MYC
FEATURES             Location/Qualifiers
     source          1..6085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    151..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(494..1288)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(605..624)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1215..1234
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    1773..1821
                     /note="synthetic polyadenylation signal"
     misc_feature    1835..1926
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1875..1894
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2200..2209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2206..3858
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2272..2290)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3939..4020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4268..4285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4439..5027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4519..4538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5227..6018,6019..6085,1..2))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atactcgtcc tttttcaata ttattgaagc atttatcagg gttacactgc ggctcctcat
       61 cgcgataata aaccccagta aatgctttta aaaaacctcc cacacctccc cctgaacctg
      121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      241 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtggaaaacg ttcttcgggg
      301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
      361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
      421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      481 ttcctttttc aattcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
      541 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
      601 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
      661 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
      721 cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca
      781 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
      841 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
      901 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
      961 caggagcaag gtgggatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     1021 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     1081 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
     1141 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     1201 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     1261 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
     1321 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     1381 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     1441 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     1501 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     1561 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     1621 aattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1681 cgaaatcggc aaaatccctt ataaatcaaa agagtacgtc tctcaaggat aagtaagtaa
     1741 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     1801 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     1861 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     1921 ttctctggcc taactggccg gtacctgagc tcgctagcct cgaggatatc aagatctggc
     1981 ctcggcggcc aagcttaccc ccgagctgtg ctgctcgcgg ccgccaccgc cgggccccgg
     2041 ccgtccctgg ctcccctcct gcctcgagaa gggcagggct tctcagaggc ttggcgggaa
     2101 aaagaacgga gggagggatc gcgctgagta taaaagccgg ttttcggggc tttatctaac
     2161 tcgctgtagt aattccggca atccggtact gttggtaaag ccaccatgga agacgccaaa
     2221 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
     2281 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
     2341 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
     2401 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
     2461 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
     2521 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg
     2581 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc
     2641 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
     2701 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc
     2761 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct
     2821 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct
     2881 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
     2941 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     3001 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg
     3061 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat
     3121 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa
     3181 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact
     3241 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     3301 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     3361 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg
     3421 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     3481 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa
     3541 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc
     3601 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     3661 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     3721 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     3781 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     3841 ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     3901 cgatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3961 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     4021 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     4081 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     4141 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     4201 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     4261 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4321 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4381 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4441 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4501 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4561 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4621 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4681 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4741 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4801 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4861 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4921 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4981 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5041 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5101 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5161 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     5221 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     5281 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     5341 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     5401 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     5461 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     5521 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     5581 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     5641 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     5701 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     5761 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     5821 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     5881 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     5941 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     6001 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     6061 tgcgacacga aaatgttgga tgctc
//
