LOCUS       sequence-193115-        8842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for untagged human CXCR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-CXCR4
FEATURES             Location/Qualifiers
     source          1..8842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(722..740)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        808..912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(913..981,982..1773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1131..1150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1944..2532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2433..2452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2963..3108
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3151..4188
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4186..4205)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4230..4278
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4932..5311
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5312..5515
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5466..5486
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5512..5536
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5564..5573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6293..6304
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6651..6785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6675..6694)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6729..6748
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6928..6950)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cccgctcctg ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga
       61 cgtttttggg gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg
      121 gctcctggtc ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca
      181 ccccctcttc tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc
      241 tctcctaggc catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa
      301 agagatcaat agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct
      361 gccccctcca acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag
      421 gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc
      481 ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa
      541 ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc
      601 ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc
      661 ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag
      721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1141 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1441 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     2101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2581 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     2641 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     2701 gcgaacgcca gcaagacgta gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc
     2761 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc
     2821 tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc
     2881 ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata
     2941 tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa
     3001 ttcgaacacg cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt
     3061 gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag
     3121 cgtgccgcag atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct
     3181 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     3241 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     3301 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     3361 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     3421 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     3481 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     3541 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     3601 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     3661 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     3721 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     3781 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     3841 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     3901 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     3961 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     4021 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     4081 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     4141 cgacgcccca gcactcgtcc ggatcgggag atgggggagg ctaactgaaa cacggaagga
     4201 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     4261 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     4321 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     4381 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     4441 tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt tatggttcgt
     4501 gggggttatt attttgggcg ttgcgtgggg tcaggtccac gactggactg agcagacaga
     4561 cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg
     4621 gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg
     4681 ccaagctagt cgaccaattc tcatgtttga cagcttatca tcgcagatcc gggcaacgtt
     4741 gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat tgaatcaata
     4801 ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg gctattggcc
     4861 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
     4921 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     4981 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     5041 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     5101 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     5161 ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa
     5221 atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta
     5281 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg
     5341 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     5401 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc
     5461 gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     5521 agtgaaccgt cagatctcta gaagctgggt accagctgct agcgccacca tggaggggat
     5581 cagtatatac acttcagata actacaccga ggaaatgggc tcaggggact atgactccat
     5641 gaaggaaccc tgtttccgtg aagaaaatgc taatttcaat aaaatcttcc tgcccaccat
     5701 ctactccatc atcttcttaa ctggcattgt gggcaatgga ttggtcatcc tggtcatggg
     5761 ttaccagaag aaactgagaa gcatgacgga caagtacagg ctgcacctgt cagtggccga
     5821 cctcctcttt gtcatcacgc ttcccttctg ggcagttgat gccgtggcaa actggtactt
     5881 tgggaacttc ctatgcaagg cagtccatgt catctacaca gtcaacctct acagcagtgt
     5941 cctcatcctg gccttcatca gtctggaccg ctacctggcc atcgtccacg ccaccaacag
     6001 tcagaggcca aggaagctgt tggctgaaaa ggtggtctat gttggcgtct ggatccctgc
     6061 cctcctgctg actattcccg acttcatctt tgccaacgtc agtgaggcag atgacagata
     6121 tatctgtgac cgcttctacc ccaatgactt gtgggtggtt gtgttccagt ttcagcacat
     6181 catggttggc cttatcctgc ctggtattgt catcctgtcc tgctattgca ttatcatctc
     6241 caagctgtca cactccaagg gccaccagaa gcgcaaggcc ctcaagacca cagtcatcct
     6301 catcctggct ttcttcgcct gttggctgcc ttactacatt gggatcagca tcgactcctt
     6361 catcctcctg gaaatcatca agcaagggtg tgagtttgag aacactgtgc acaagtggat
     6421 ttccatcacc gaggccctag ctttcttcca ctgttgtctg aaccccatcc tctatgcttt
     6481 ccttggagcc aaatttaaaa cctctgccca gcacgcactc acctctgtga gcagagggtc
     6541 cagcctcaag atcctctcca aaggaaagcg aggtggacat tcatctgttt ccactgagtc
     6601 tgagtcttca agttttcact ccagctaata gctcgaggcc ggcaaggccg gatccagaca
     6661 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     6721 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     6781 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     6841 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct
     6901 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     6961 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     7021 tggcgggtgt cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac
     7081 gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct
     7141 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     7201 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     7261 ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac
     7321 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     7381 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     7441 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     7501 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     7561 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     7621 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     7681 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     7741 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     7801 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     7861 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     7921 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     7981 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     8041 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     8101 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     8161 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     8221 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     8281 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     8341 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     8401 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     8461 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     8521 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
     8581 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
     8641 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     8701 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     8761 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     8821 tatgctatcc taatctgtat cc
//
