LOCUS       sequence-193116-        8545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for untagged human CCR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-CCR5
FEATURES             Location/Qualifiers
     source          1..8545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(621..639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        707..811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(812..880,881..1672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1030..1049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1843..2431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2332..2351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2862..3007
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3050..4087
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4085..4104)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4129..4177
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4831..5210
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5211..5414
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5365..5385
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5411..5435
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5463..5472
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5619..5630
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6550..6684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6574..6593)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6628..6647
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6827..6849)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg
       61 tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg
      121 tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc
      181 agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat
      241 acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt
      301 cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat
      361 caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag
      421 cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt
      481 ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg
      541 aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga
      601 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      661 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      781 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      841 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      901 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      961 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1021 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     1081 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1141 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1201 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1261 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1321 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     1381 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1441 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1501 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1561 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1621 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1681 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1741 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1981 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2461 cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     2521 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     2581 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag
     2641 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     2701 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     2761 ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg
     2821 tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca
     2881 gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt
     2941 ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc
     3001 cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc
     3061 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     3121 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     3181 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     3241 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     3301 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     3361 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     3421 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     3481 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     3541 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     3601 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     3661 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     3721 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     3781 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     3841 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     3901 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     3961 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     4021 actcgccgat agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc
     4081 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     4141 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     4201 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     4261 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     4321 ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg
     4381 ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg
     4441 actggactga gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact
     4501 ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac
     4561 cgcgcggatt tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat
     4621 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     4681 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     4741 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     4801 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
     4861 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     4921 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     4981 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     5041 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     5101 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     5161 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     5221 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     5281 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     5341 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     5401 atataagcag agctcgttta gtgaaccgtc agatctctag aagctgggta ccagctgcta
     5461 gcgccaccat ggattatcaa gtgtcaagtc caatctatga catcaattat tatacatcgg
     5521 agccctgcca aaaaatcaat gtgaagcaaa tcgcagcccg cctcctgcct ccgctctact
     5581 cactggtgtt catctttggt tttgtgggca acatgctggt catcctcatc ctgataaact
     5641 gcaaaaggct gaagagcatg actgacatct acctgctcaa cctggccatc tctgacctgt
     5701 ttttccttct tactgtcccc ttctgggctc actatgctgc cgcccagtgg gactttggaa
     5761 atacaatgtg tcaactcttg acagggctct attttatagg cttcttctct ggaatcttct
     5821 tcatcatcct cctgacaatc gataggtacc tggctgtcgt ccatgctgtg tttgctttaa
     5881 aagccaggac ggtcaccttt ggggtggtga caagtgtgat cacttgggtg gtggctgtgt
     5941 ttgcgtctct cccaggaatc atctttacca gatctcaaaa agaaggtctt cattacacct
     6001 gcagctctca ttttccatac agtcagtatc aattctggaa gaatttccag acattaaaga
     6061 tagtcatctt ggggctggtc ctgccgctgc ttgtcatggt catctgctac tcgggaatcc
     6121 taaaaactct gcttcggtgt cgaaatgaga agaagaggca cagggctgtg aggcttatct
     6181 tcaccatcat gattgtttat tttctcttct gggctcccta caacattgtc cttctcctga
     6241 acaccttcca ggaattcttt ggcctgaata attgcagtag ctctaacagg ttggaccaag
     6301 ctatgcaggt gacagagact cttgggatga cgcactgctg catcaacccc atcatctatg
     6361 cctttgtcgg ggagaagttc agaaactacc tcttagtctt cttccaaaag cacattgcca
     6421 aacgcttctg caaatgctgt tctattttcc agcaagaggc tcccgagcga gcaagctcag
     6481 tttacacccg atccactggg gagcaggaaa tatctgtggg cttgtgataa ctcgaggccg
     6541 gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6601 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6661 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     6721 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     6781 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6841 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     6901 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg
     6961 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     7021 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     7081 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     7141 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     7201 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     7261 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     7321 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     7381 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     7441 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     7501 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     7561 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     7621 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     7681 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     7741 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     7801 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     7861 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     7921 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     7981 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     8041 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     8101 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     8161 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     8221 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     8281 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     8341 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     8401 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     8461 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     8521 cccaaatatc tggatagcat atgct
//
