LOCUS       sequence-193121-       10334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal FLAG-tagged human CXCR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-FLAG-CXCR4
FEATURES             Location/Qualifiers
     source          1..10334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(735..753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        821..925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(926..994,995..1786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1144..1163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1957..2545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2446..2465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2976..3121
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3164..4201
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4199..4218)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4243..4291
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4945..5324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5325..5528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5479..5499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5525..5549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6390..6401
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6748..6882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6772..6791)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6826..6845
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7025..7047)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7607..9396
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc
       61 aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc
      121 tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg
      181 tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga
      241 tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat
      301 gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg
      361 agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt
      421 cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa
      481 ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa
      541 gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg
      601 acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa
      661 agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc
      721 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      781 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1081 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1141 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     1201 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1261 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1321 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1381 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1441 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     1501 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1561 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1621 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1681 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1741 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1801 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1861 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2101 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2401 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2461 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2521 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2581 cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     2641 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     2701 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc
     2761 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
     2821 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     2881 gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc
     2941 cggaagaaat atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct
     3001 tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt
     3061 cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta
     3121 acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact
     3181 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     3241 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     3301 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     3361 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     3421 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     3481 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     3541 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     3601 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     3661 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     3721 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     3781 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     3841 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     3901 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     3961 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     4021 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     4081 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     4141 cgatagtgga aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg
     4201 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     4261 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     4321 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     4381 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     4441 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg
     4501 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga
     4561 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg
     4621 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg
     4681 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     4741 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     4801 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     4861 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     4921 catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     4981 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5041 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5101 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5161 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     5221 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     5281 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5341 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5401 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5461 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5521 gcagagctcg tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca
     5581 ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc gactacaagg
     5641 atgacgacga cggcagcccc ggaggagctg aggggatcag tatatacact tcagataact
     5701 acaccgagga aatgggctca ggggactatg actccatgaa ggaaccctgt ttccgtgaag
     5761 aaaatgctaa tttcaataaa atcttcctgc ccaccatcta ctccatcatc ttcttaactg
     5821 gcattgtggg caatggattg gtcatcctgg tcatgggtta ccagaagaaa ctgagaagca
     5881 tgacggacaa gtacaggctg cacctgtcag tggccgacct cctctttgtc atcacgcttc
     5941 ccttctgggc agttgatgcc gtggcaaact ggtactttgg gaacttccta tgcaaggcag
     6001 tccatgtcat ctacacagtc aacctctaca gcagtgtcct catcctggcc ttcatcagtc
     6061 tggaccgcta cctggccatc gtccacgcca ccaacagtca gaggccaagg aagctgttgg
     6121 ctgaaaaggt ggtctatgtt ggcgtctgga tccctgccct cctgctgact attcccgact
     6181 tcatctttgc caacgtcagt gaggcagatg acagatatat ctgtgaccgc ttctacccca
     6241 atgacttgtg ggtggttgtg ttccagtttc agcacatcat ggttggcctt atcctgcctg
     6301 gtattgtcat cctgtcctgc tattgcatta tcatctccaa gctgtcacac tccaagggcc
     6361 accagaagcg caaggccctc aagaccacag tcatcctcat cctggctttc ttcgcctgtt
     6421 ggctgcctta ctacattggg atcagcatcg actccttcat cctcctggaa atcatcaagc
     6481 aagggtgtga gtttgagaac actgtgcaca agtggatttc catcaccgag gccctagctt
     6541 tcttccactg ttgtctgaac cccatcctct atgctttcct tggagccaaa tttaaaacct
     6601 ctgcccagca cgcactcacc tctgtgagca gagggtccag cctcaagatc ctctccaaag
     6661 gaaagcgagg tggacattca tctgtttcca ctgagtctga gtcttcaagt tttcactcca
     6721 gctaatagct cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt
     6781 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     6841 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     6901 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     6961 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     7021 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7081 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     7141 tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     7201 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     7261 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     7321 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     7381 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     7441 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     7501 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     7561 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     7621 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     7681 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     7741 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     7801 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     7861 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     7921 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     7981 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     8041 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     8101 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     8161 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     8221 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     8281 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     8341 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     8401 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     8461 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     8521 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     8581 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     8641 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     8701 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     8761 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     8821 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     8881 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     8941 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     9001 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     9061 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     9121 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     9181 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9241 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
     9301 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
     9361 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
     9421 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
     9481 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
     9541 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
     9601 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
     9661 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
     9721 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
     9781 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
     9841 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
     9901 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
     9961 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
    10021 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
    10081 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
    10141 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
    10201 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
    10261 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
    10321 ctatcgctcc cggg
//
