LOCUS       sequence-193123-       10161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal myc-tagged human CCR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-myc-CCR5
FEATURES             Location/Qualifiers
     source          1..10161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(730..748)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        816..920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(921..989,990..1781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1139..1158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1952..2540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2441..2460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2971..3116
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3159..4196
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4194..4213)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4238..4286
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4940..5319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5320..5523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5474..5494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5520..5544
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5626..5655
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    5821..5832
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6752..6886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6776..6795)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6830..6849
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7029..7051)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7611..9400
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc
       61 aacttggacg tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc
      121 cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat
      181 ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt
      241 ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc
      301 acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc
      361 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac
      421 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact
      481 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag
      541 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc
      601 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg
      661 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa
      721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1141 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     1201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1441 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     1501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2581 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     2641 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     2701 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt
     2761 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     2821 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     2881 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     2941 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     3001 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat
     3061 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
     3121 gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg
     3181 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     3241 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     3301 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     3361 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     3421 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     3481 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     3541 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     3601 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     3661 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     3721 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     3781 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     3841 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     3901 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     3961 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     4021 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     4081 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     4141 gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca
     4201 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     4261 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     4321 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     4381 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     4441 ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta
     4501 tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag
     4561 cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg
     4621 aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt
     4681 ctggcgtgcc aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     4741 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     4801 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     4861 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     4921 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     4981 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     5041 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     5101 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     5161 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     5221 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     5281 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     5341 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     5401 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     5461 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     5521 gctcgtttag tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg
     5581 aagaccatca tcgccctgag ctacatcttc tgcctggtgt tcgccgagca aaagctgatc
     5641 tcagaggagg acctgggcag ccctggcggt gccgattatc aagtgtcaag tccaatctat
     5701 gacatcaatt attatacatc ggagccctgc caaaaaatca atgtgaagca aatcgcagcc
     5761 cgcctcctgc ctccgctcta ctcactggtg ttcatctttg gttttgtggg caacatgctg
     5821 gtcatcctca tcctgataaa ctgcaaaagg ctgaagagca tgactgacat ctacctgctc
     5881 aacctggcca tctctgacct gtttttcctt cttactgtcc ccttctgggc tcactatgct
     5941 gccgcccagt gggactttgg aaatacaatg tgtcaactct tgacagggct ctattttata
     6001 ggcttcttct ctggaatctt cttcatcatc ctcctgacaa tcgataggta cctggctgtc
     6061 gtccatgctg tgtttgcttt aaaagccagg acggtcacct ttggggtggt gacaagtgtg
     6121 atcacttggg tggtggctgt gtttgcgtct ctcccaggaa tcatctttac cagatctcaa
     6181 aaagaaggtc ttcattacac ctgcagctct cattttccat acagtcagta tcaattctgg
     6241 aagaatttcc agacattaaa gatagtcatc ttggggctgg tcctgccgct gcttgtaatg
     6301 gtcatctgct actcgggaat cctaaaaact ctgcttcggt gtcgaaatga gaagaagagg
     6361 cacagggctg tgaggcttat cttcaccatc atgattgttt attttctctt ctgggctccc
     6421 tacaacattg tccttctcct gaacaccttc caggaattct ttggcctgaa taattgcagt
     6481 agctctaaca ggttggacca agctatgcag gtgacagaga ctcttgggat gacgcactgc
     6541 tgcatcaacc ccatcatcta tgcctttgtc ggggagaagt tcagaaacta cctcttagtc
     6601 ttcttccaaa agcacattgc caaacgcttc tgcaaatgct gttctatttt ccagcaagag
     6661 gctcccgagc gagcaagctc agtttacacc cgatccactg gggagcagga aatatctgtg
     6721 ggcttgtgat aactcgaggc cggcaaggcc ggatccagac atgataagat acattgatga
     6781 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     6841 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     6901 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     6961 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
     7021 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     7081 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     7141 gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     7201 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     7261 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     7321 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
     7381 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
     7441 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
     7501 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
     7561 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
     7621 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
     7681 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
     7741 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
     7801 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
     7861 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
     7921 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
     7981 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
     8041 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
     8101 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
     8161 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
     8221 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
     8281 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
     8341 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
     8401 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
     8461 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
     8521 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
     8581 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
     8641 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
     8701 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     8761 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     8821 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     8881 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     8941 tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata
     9001 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     9061 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     9121 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     9181 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     9241 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca
     9301 gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct
     9361 cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag
     9421 gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt
     9481 cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg
     9541 ggtatgccca attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa
     9601 atacaccaac agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca
     9661 cggcttttaa tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca
     9721 ccctcatctc catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc
     9781 ccttccacca taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca
     9841 gtcaccctga tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc
     9901 ttcaaaacct cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac
     9961 tcccttagcg ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc
    10021 aatggtgtaa gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg
    10081 gaggtcttac tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc
    10141 ctttctacgt gactcctagc c
//
