LOCUS       sequence-193127-       10022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 
            mutant W161Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-myc-CXCR4-W161Y
FEATURES             Location/Qualifiers
     source          1..10022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(723..741)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        809..913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(914..982,983..1774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1132..1151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1945..2533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2434..2453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2964..3109
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3152..4189
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4187..4206)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4231..4279
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4933..5312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5313..5516
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5467..5487
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5513..5537
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5619..5648
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6387..6398
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6745..6879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6769..6788)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6823..6842
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7022..7044)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7604..9393
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
       61 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
      121 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
      181 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
      241 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
      301 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
      361 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
      421 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
      481 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
      541 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
      601 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
      661 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
      721 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      781 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      841 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      901 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      961 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1021 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1081 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1141 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1201 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1261 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1321 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1381 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1441 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1501 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1561 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1621 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1681 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1741 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1801 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1861 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1921 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1981 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2041 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2101 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2161 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2221 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2281 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2341 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2401 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2461 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2521 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2581 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     2641 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     2701 cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg
     2761 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     2821 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     2881 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
     2941 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
     3001 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
     3061 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
     3121 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     3181 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     3241 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     3301 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     3361 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     3421 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     3481 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     3541 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     3601 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     3661 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     3721 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     3781 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     3841 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     3901 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     3961 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     4021 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     4081 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     4141 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
     4201 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4261 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4321 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4381 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4441 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
     4501 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
     4561 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
     4621 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
     4681 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     4741 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     4801 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     4861 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     4921 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     4981 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     5041 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     5101 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     5161 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
     5221 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
     5281 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
     5341 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5401 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
     5461 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     5521 tagtgaaccg tcagatctct agaagctggg taccagctgc tagcgccacc atgaagacca
     5581 tcatcgccct gagctacatc ttctgcctgg tgttcgccga gcagaaattg atcagcgagg
     5641 aggacctggg cagccctgga ggcgccgagg ggatcagtat atacacttca gataactaca
     5701 ccgaggaaat gggctcaggg gactatgact ccatgaagga accctgtttc cgtgaagaaa
     5761 atgctaattt caataaaatc ttcctgccca ccatctactc catcatcttc ttaactggca
     5821 ttgtgggcaa tggattggtc atcctggtca tgggttacca gaagaaactg agaagcatga
     5881 cggacaagta caggctgcac ctgtcagtgg ccgacctcct ctttgtcatc acgcttccct
     5941 tctgggcagt tgatgccgtg gcaaactggt actttgggaa cttcctatgc aaggcagtcc
     6001 atgtcatcta cacagtcaac ctctacagca gtgtcctcat cctggccttc atcagtctgg
     6061 accgctacct ggccatcgtc cacgccacca acagtcagag gccaaggaag ctgttggctg
     6121 aaaaggtggt ctatgttggc gtctatatcc ctgccctcct gctgactatt cccgacttca
     6181 tctttgccaa cgtcagtgag gcagatgaca gatatatctg tgaccgcttc taccccaatg
     6241 acttgtgggt ggttgtgttc cagtttcagc acatcatggt tggccttatc ctgcctggta
     6301 ttgtcatcct gtcctgctat tgcattatca tctccaagct gtcacactcc aagggccacc
     6361 agaagcgcaa ggccctcaag accacagtca tcctcatcct ggctttcttc gcctgttggc
     6421 tgccttacta cattgggatc agcatcgact ccttcatcct cctggaaatc atcaagcaag
     6481 ggtgtgagtt tgagaacact gtgcacaagt ggatttccat caccgaggcc ctagctttct
     6541 tccactgttg tctgaacccc atcctctatg ctttccttgg agccaaattt aaaacctctg
     6601 cccagcacgc actcacctct gtgagcagag ggtccagcct caagatcctc tccaaaggaa
     6661 agcgaggtgg acattcatct gtttccactg agtctgagtc ttcaagtttt cactccagct
     6721 aatagctcga ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga
     6781 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6841 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6901 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     6961 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     7021 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7081 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     7141 ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     7201 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     7261 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     7321 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     7381 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     7441 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     7501 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     7561 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     7621 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     7681 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     7741 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     7801 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     7861 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     7921 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     7981 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     8041 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     8101 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     8161 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     8221 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     8281 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     8341 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     8401 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     8461 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     8521 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     8581 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     8641 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     8701 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     8761 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     8821 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     8881 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     8941 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     9001 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     9061 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     9121 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     9181 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     9241 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
     9301 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
     9361 gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt
     9421 taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat
     9481 gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc
     9541 ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc
     9601 aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt
     9661 taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat
     9721 ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca
     9781 ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc
     9841 tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa
     9901 cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta
     9961 gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg
    10021 ta
//
