LOCUS       sequence-193128-       10112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal HA-tagged human CCR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-HA-CCR5
FEATURES             Location/Qualifiers
     source          1..10112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(691..709)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        777..881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(882..950,951..1742)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1100..1119)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1913..2501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2402..2421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2932..3077
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3120..4157
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4155..4174)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4199..4247
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4901..5280
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5281..5484
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5435..5455
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5481..5505
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5587..5613
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5587..5613
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5779..5790
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6710..6844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6734..6753)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6788..6807
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6987..7009)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7569..9358
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat
       61 gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc
      121 ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg
      181 agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg
      241 gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg
      301 ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc
      361 ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc
      421 tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt
      481 gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg
      541 gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact
      601 ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg
      661 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
      721 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      781 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      841 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      901 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      961 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1021 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1081 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     1141 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1201 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1261 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1321 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1381 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     1441 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1501 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1561 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1621 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1681 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1741 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1801 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1861 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1921 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1981 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2041 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2101 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2161 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2221 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2281 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2341 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2401 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2461 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2521 ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct
     2581 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     2641 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     2701 cggccccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg
     2761 ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg
     2821 agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt
     2881 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
     2941 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     3001 ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     3061 tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg caatgagata
     3121 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     3181 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     3241 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     3301 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     3361 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     3421 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     3481 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     3541 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     3601 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     3661 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     3721 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     3781 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     3841 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     3901 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     3961 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     4021 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     4081 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg gatcgggaga
     4141 tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4201 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt
     4261 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     4321 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     4381 agccaacgtc ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg
     4441 tcccccatgg ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt
     4501 caggtccacg actggactga gcagacagac ccatggtttt tggatggcct gggcatggac
     4561 cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc
     4621 caaaaaccac cgcgcggatt tctggcgtgc caagctagtc gaccaattct catgtttgac
     4681 agcttatcat cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag
     4741 gtatggaaga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat
     4801 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     4861 tacatttata ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt
     4921 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4981 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5041 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5101 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5161 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5221 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5281 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     5341 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5401 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     5461 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag aagctgggta
     5521 ccagctgcta gcgccaccat gaagaccatc atcgccctga gctacatctt ctgcctggtg
     5581 ttcgcctacc cctacgacgt gcccgactac gccggctccc cgggaggcgc ggattatcaa
     5641 gtgtcaagtc caatctatga catcaattat tatacatcgg agccctgcca aaaaatcaat
     5701 gtgaagcaaa tcgcagcccg cctcctgcct ccgctctact cactggtgtt catctttggt
     5761 tttgtgggca acatgctggt catcctcatc ctgataaact gcaaaaggct gaagagcatg
     5821 actgacatct acctgctcaa cctggccatc tctgacctgt ttttccttct tactgtcccc
     5881 ttctgggctc actatgctgc cgcccagtgg gactttggaa atacaatgtg tcaactcttg
     5941 acagggctct attttatagg cttcttctct ggaatcttct tcatcatcct cctgacaatc
     6001 gataggtacc tggctgtcgt ccatgctgtg tttgctttaa aagccaggac ggtcaccttt
     6061 ggggtggtga caagtgtgat cacttgggtg gtggctgtgt ttgcgtctct cccaggaatc
     6121 atctttacca gatctcaaaa agaaggtctt cattacacct gcagctctca ttttccatac
     6181 agtcagtatc aattctggaa gaatttccag acattaaaga tagtcatctt ggggctggtc
     6241 ctgccgctgc ttgtcatggt catctgctac tcgggaatcc taaaaactct gcttcggtgt
     6301 cgaaatgaga agaagaggca cagggctgtg aggcttatct tcaccatcat gattgtttat
     6361 tttctcttct gggctcccta caacattgtc cttctcctga acaccttcca ggaattcttt
     6421 ggcctgaata attgcagtag ctctaacagg ttggaccaag ctatgcaggt gacagagact
     6481 cttgggatga cgcactgctg catcaacccc atcatctatg cctttgtcgg ggagaagttc
     6541 agaaactacc tcttagtctt cttccaaaag cacattgcca aacgcttctg caaatgctgt
     6601 tctattttcc agcaagaggc tcccgagcga gcaagctcag tttacacccg atccactggg
     6661 gagcaggaaa tatctgtggg cttgtgataa ctcgaggccg gcaaggccgg atccagacat
     6721 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     6781 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     6841 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     6901 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc
     6961 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7021 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7081 ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatcccc gccccggacg
     7141 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     7201 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     7261 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     7321 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     7381 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     7441 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     7501 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     7561 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     7621 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     7681 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     7741 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     7801 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     7861 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     7921 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     7981 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     8041 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     8101 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     8161 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     8221 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     8281 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     8341 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     8401 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     8461 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     8521 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     8581 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     8641 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     8701 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     8761 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     8821 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     8881 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     8941 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     9001 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     9061 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     9121 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     9181 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     9241 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
     9301 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
     9361 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
     9421 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
     9481 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
     9541 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
     9601 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
     9661 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
     9721 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
     9781 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
     9841 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
     9901 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
     9961 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    10021 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    10081 gttccttcgt cggtagtcct ttctacgtga ct
//
