LOCUS       sequence-193132-       10477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 
            mutant H79Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-myc-CXCR4-H79Q
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(721..739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(912..980,981..1772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1130..1149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2432..2451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2962..3107
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3150..4187
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4185..4204)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4229..4277
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4931..5310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5311..5514
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5465..5485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5511..5535
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5617..5646
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6385..6396
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6743..6877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6767..6786)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6821..6840
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7020..7042)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7602..9391
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
       61 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
      121 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
      181 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
      241 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
      301 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
      361 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
      421 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
      481 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
      541 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
      601 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
      661 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
      721 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      961 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1021 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1081 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1141 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1201 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1261 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1321 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1381 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1441 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1501 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1561 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1621 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1681 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1741 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1801 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1861 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1921 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1981 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2041 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2101 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2161 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2281 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2341 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2401 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2461 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2521 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2581 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     2641 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     2701 cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct
     2761 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     2821 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc
     2881 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     2941 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     3001 tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg
     3061 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc
     3121 gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     3181 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     3241 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     3301 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     3361 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     3421 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     3481 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     3541 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3601 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3661 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     3721 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3781 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3841 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     3901 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     3961 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     4021 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     4081 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     4141 gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag
     4201 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     4261 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     4321 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     4381 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4441 agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
     4501 ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
     4561 ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
     4621 cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc
     4681 caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
     4741 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
     4801 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     4861 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     4921 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4981 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5041 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5101 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5161 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     5221 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     5281 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     5341 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5401 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     5461 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     5521 gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc
     5581 atcgccctga gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag
     5641 gacctgggca gccctggagg cgccgagggg atcagtatat acacttcaga taactacacc
     5701 gaggaaatgg gctcagggga ctatgactcc atgaaggaac cctgtttccg tgaagaaaat
     5761 gctaatttca ataaaatctt cctgcccacc atctactcca tcatcttctt aactggcatt
     5821 gtgggcaatg gattggtcat cctggtcatg ggttaccaga agaaactgag aagcatgacg
     5881 gacaagtaca ggctgcagct gtcagtggcc gacctcctct ttgtcatcac gcttcccttc
     5941 tgggcagttg atgccgtggc aaactggtac tttgggaact tcctatgcaa ggcagtccat
     6001 gtcatctaca cagtcaacct ctacagcagt gtcctcatcc tggccttcat cagtctggac
     6061 cgctacctgg ccatcgtcca cgccaccaac agtcagaggc caaggaagct gttggctgaa
     6121 aaggtggtct atgttggcgt ctggatccct gccctcctgc tgactattcc cgacttcatc
     6181 tttgccaacg tcagtgaggc agatgacaga tatatctgtg accgcttcta ccccaatgac
     6241 ttgtgggtgg ttgtgttcca gtttcagcac atcatggttg gccttatcct gcctggtatt
     6301 gtcatcctgt cctgctattg cattatcatc tccaagctgt cacactccaa gggccaccag
     6361 aagcgcaagg ccctcaagac cacagtcatc ctcatcctgg ctttcttcgc ctgttggctg
     6421 ccttactaca ttgggatcag catcgactcc ttcatcctcc tggaaatcat caagcaaggg
     6481 tgtgagtttg agaacactgt gcacaagtgg atttccatca ccgaggccct agctttcttc
     6541 cactgttgtc tgaaccccat cctctatgct ttccttggag ccaaatttaa aacctctgcc
     6601 cagcacgcac tcacctctgt gagcagaggg tccagcctca agatcctctc caaaggaaag
     6661 cgaggtggac attcatctgt ttccactgag tctgagtctt caagttttca ctccagctaa
     6721 tagctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     6781 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6841 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6901 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6961 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     7021 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7081 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     7141 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     7201 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     7261 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     7321 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     7381 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     7441 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     7501 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     7561 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     7621 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     7681 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     7741 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     7801 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     7861 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     7921 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     7981 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     8041 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     8101 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     8161 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     8221 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     8281 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     8341 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     8401 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     8461 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     8521 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     8581 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     8641 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     8701 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     8761 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     8821 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     8881 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     8941 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     9001 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     9061 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     9121 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     9181 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     9241 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     9301 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     9361 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     9421 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     9481 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     9541 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     9601 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     9661 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     9721 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     9781 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     9841 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     9901 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     9961 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
    10021 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
    10081 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
    10141 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
    10201 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
    10261 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
    10321 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
    10381 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
    10441 gacccggatg atgatgactg actactggga ctcctgg
//
