LOCUS       sequence-193136-       10229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for myc-tagged human CCR5 mutant 
            R5gp120-Hi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-CCR5(R5gp120-Hi)
FEATURES             Location/Qualifiers
     source          1..10229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(714..732)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        800..904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(905..973,974..1765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1123..1142)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1936..2524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2425..2444
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2955..3100
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3143..4180
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4178..4197)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4222..4270
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4924..5303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5304..5507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5458..5478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5504..5528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5610..5639
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6736..6870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6760..6779)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6814..6833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7013..7035)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7595..9384
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgctcctgct cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg
       61 gggtctccgg acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg
      121 tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct
      181 tctttgaggt ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag
      241 gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca
      301 atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc
      361 caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa
      421 attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact
      481 cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt
      541 cccccttttt gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa
      601 ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg
      661 atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
      721 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      781 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      901 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      961 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1021 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1081 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1141 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1201 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1261 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1321 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1381 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1441 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1501 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1561 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1621 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1681 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1741 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1801 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1861 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1921 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1981 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2041 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     2101 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2161 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2221 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2281 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2341 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2401 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2521 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg
     2581 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
     2641 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
     2701 cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg
     2761 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     2821 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccctgcttca
     2881 tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg
     2941 tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca
     3001 cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg
     3061 tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc
     3121 agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     3181 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     3241 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     3301 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     3361 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     3421 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     3481 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     3541 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     3601 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     3661 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     3721 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     3781 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     3841 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     3901 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     3961 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     4021 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     4081 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     4141 cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag gagacaatac
     4201 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
     4261 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4321 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4381 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagccact
     4441 ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc gtgggggtta
     4501 ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca gacccatggt
     4561 ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc gggcgtctgt
     4621 ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg tgccaagcta
     4681 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     4741 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     4801 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     4861 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
     4921 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     4981 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     5041 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     5101 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     5161 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
     5221 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
     5281 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
     5341 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     5401 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
     5461 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     5521 gtcagatctc tagaagctgg gtaccagctg ctagcgccac catgaagacc atcatcgccc
     5581 tgagctacat cttctgcctg gtgttcgccg agcaaaagct gatctcagag gaggacctgg
     5641 gcagccctgg cggtgccgat tatcaagtgt ctagtcttga gtatgacatg aattattata
     5701 cagaggagga ctgcgagaaa atcaatgtga agcaaatcgc agcccgcctc ctgcctccgc
     5761 tctactcact ggtgttcatc tttggttttg tgggcaacat gctgaacatc ctcatcctga
     5821 taaactgcaa aaggctgaag agcatgactg acatctacct gctcaacctg gccatctctg
     5881 acctgttttt cgctcttact gtccccttct gggctcacta tgctgccgcc cagtgggact
     5941 ttggaaatac aatgtgtcaa ctcttgacag ggctctattt tataggcttc ttctctggaa
     6001 tcttcttcat catcctcctg acaatcgata ggtacctggc tgtcgtccat gctgtgtttg
     6061 ctttaaaagc caggacggtc acctttgggg tggtgacaag tgtgatcact tgggtggtgg
     6121 ctgtgtttgc gtctctccca ggaatcatct ttaccagatc tcaaaaagaa ggtcttcatt
     6181 acacctgcag ctctcatttt ccatacagtc agtatcaatt ctggaagaat ttccagacat
     6241 taaagatagt catcttgggg ctggtcctgc cgctgcttgt aatggtcatc tgctactcgg
     6301 gaatcctaaa aactctgctt cggtgtcgaa atgagaagaa gaggcacagg gctgtgaggc
     6361 ttatcttcac catcatgatt gtttattttc tcttctgggc tccctacaac attgtccttc
     6421 tcctgaacac cttccaggaa ttctttggcc tgaataattg cgagagctct aacaggttgg
     6481 accaagctat gcaggtgaca gagactcttg ggatgacgca ctgctgcatc aaccccatca
     6541 tctatgcctt tgtcggggag aagttcagaa actacctctt agtcttcttc caaaagcaca
     6601 ttgccaaacg cttctgcaaa tgctgttcta ttttccagca agaggctccc gagcgagcaa
     6661 gctcagttta cacccgatcc actggggagc aggaaatatc tgtgggcttg tgataactcg
     6721 aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     6781 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6841 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     6901 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     6961 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     7021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc
     7141 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     7201 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     7261 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     7321 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     7381 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     7441 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     7501 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     7561 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     7621 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     7681 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     7741 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     7801 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     7861 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     7921 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     7981 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     8041 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     8101 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     8161 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     8221 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     8281 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     8341 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     8401 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     8461 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     8521 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     8581 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     8641 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     8701 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     8761 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     8821 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     8881 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     8941 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     9001 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     9061 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     9121 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     9181 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     9241 ctaatctgta tccgggtagc atatgctatc ctcatgcata tacagtcagc atatgatacc
     9301 cagtagtaga gtgggagtgc tatcctttgc atatgccgcc acctcccaag ggggcgtgaa
     9361 ttttcgctgc ttgtcctttt cctgctggtt gctcccattc ttaggtgaat ttaaggaggc
     9421 caggctaaag ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa tgttttccaa
     9481 cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa catgggtatg cccaattgcc
     9541 ccatgttggg aggacgaaaa tggtgacaag acagatggcc agaaatacac caacagcacg
     9601 catgatgtct actggggatt tattctttag tgcgggggaa tacacggctt ttaatacgat
     9661 tgagggcgtc tcctaacaag ttacatcact cctgcccttc ctcaccctca tctccatcac
     9721 ctccttcatc tccgtcatct ccgtcatcac cctccgcggc agccccttcc accataggtg
     9781 gaaaccaggg aggcaaatct actccatcgt caaagctgca cacagtcacc ctgatattgc
     9841 aggtaggagc gggctttgtc ataacaaggt ccttaatcgc atccttcaaa acctcagcaa
     9901 atatatgagt ttgtaaaaag accatgaaat aacagacaat ggactccctt agcgggccag
     9961 gttgtgggcc gggtccaggg gccattccaa aggggagacg actcaatggt gtaagacgac
    10021 attgtggaat agcaagggca gttcctcgcc ttaggttgta aagggaggtc ttactacctc
    10081 catatacgaa cacaccggcg acccaagttc cttcgtcggt agtcctttct acgtgactcc
    10141 tagccaggag agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt
    10201 gaccacgatg ctttccaaac caccctcct
//
