LOCUS       sequence-193138-        9970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL 
            isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-gp120(BaL)-CD4
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(781..799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        867..971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(972..1040,1041..1832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1190..1209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2003..2591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2492..2511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3022..3167
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3210..4247
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4245..4264)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4289..4337
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4991..5370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5371..5574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5525..5545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5571..5595
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7756..7800
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7801..7824
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    7850..7984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7874..7893)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7928..7947
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8127..8149)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc
       61 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
      121 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
      181 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
      241 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
      301 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
      361 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
      421 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
      481 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
      541 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
      601 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
      661 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
      721 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
      781 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      841 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      901 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      961 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1021 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1081 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1141 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1201 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1261 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1321 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1381 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1441 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1501 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1561 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1621 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1681 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1741 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1801 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1861 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1921 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1981 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2041 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2101 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2161 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2221 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2281 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2341 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2401 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2461 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2521 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2581 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2641 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     2701 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     2761 cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct
     2821 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     2881 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc
     2941 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     3001 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     3061 tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg
     3121 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc
     3181 gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     3241 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     3301 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     3361 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     3421 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     3481 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     3541 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     3601 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3661 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3721 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     3781 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3841 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3901 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     3961 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     4021 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     4081 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     4141 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     4201 gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag
     4261 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     4321 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     4381 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     4441 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4501 agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
     4561 ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
     4621 ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
     4681 cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc
     4741 caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
     4801 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
     4861 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     4921 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     4981 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     5041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5221 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     5281 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     5341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     5401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     5521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     5581 gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat gcccatgggg
     5641 agcttgcaac cgctcgccac tctctatctc ctcggcatgt tggtagcatc tgtgctggct
     5701 gaggaaaagc tctgggtgac tgtgtattac ggggtcccag tttggaagga ggctactacc
     5761 acgctcttct gcgcctccga ccggaaggca tacgacaccg aggtgcataa tgtgtgggcc
     5821 acacacgcct gtgtgcctac cgaccccaac ccacaggaag tggaactgaa gaacgtgact
     5881 gagaatttca atatgtggaa aaataatatg gtggagcaga tgcacgagga cattattagc
     5941 ctctgggatc agtcccttaa accctgcgtg aaactgacac cgctgtgcgt aacgctgaat
     6001 tgtactgacc tccggaatgc cacgaacgga aacgacacaa ataccacgtc aagctcccga
     6061 gggatggtgg gagggggaga aatgaagaac tgctcattca atataacgac gaatatccga
     6121 gggaaggttc agaaagagta tgccctgttt tacaaactgg acatcgctcc tatcgacaac
     6181 aacagcaata acaggtaccg cctgattagc tgcaacactt ctgtgattac ccaggcctgc
     6241 cccaaggtga gcttcgagcc tatcccaatc cactactgcg ccccagcagg ctttgccatc
     6301 ctgaaatgca aggacaagaa gttcaacggc aaaggcccat gtacaaatgt cagtacagta
     6361 cagtgcacac acggtatccg cccagttgtc tctacacaac tgctgctgaa tggatccttg
     6421 gcagaggaag aggtggtgat cagatctgct aacttcgccg ataatgctaa agtcatcatt
     6481 gttcagctga acgagtctgt cgaaatcaat tgtactcgcc caaacaacaa cacgcgcaag
     6541 agcattcata tcggaccggg cagggccttt tacacaacag gggagattat tggtgatatt
     6601 agacaagccc actgcaacct cagtcgagca aagtggaatg atactctcaa caaaattgtt
     6661 atcaaattgc gggaacagtt tgggaacaag acaatcgtat ttaagcactc atccggaggc
     6721 gatccagaaa tcgtgacaca ctcttttaac tgcggtggag aatttttcta ctgcaactcc
     6781 acccagctgt tcaacagtac gtggaacgtg acagaggagt ctaacaacac cgtggagaat
     6841 aatacgatca cccttccatg tcggattaag cagatcatca atatgtggca ggaggtggga
     6901 agagcaatgt acgcgccgcc cattaggggc cagatcagat gcagttcaaa tatcacaggc
     6961 ctgctcctga cacgcgacgg aggcccagag gacaacaaga cagaggtgtt taggcccggc
     7021 ggcggcgaca tgcgagataa ttggaggtct gaattgtaca agtacaaggt ggtgaaaatc
     7081 gaacccctgg gagtggcgcc tactaaggca aagcgccgcg ttgtgcagag agagaagagg
     7141 ggatcctctg gtggcggtgg ctcgggctcc ggaggaggtg ggtcgggtgg cggcgcggct
     7201 ggcaagaagg tggtcttggg caagaagggc gataccgttg agctcacttg taccgcaagc
     7261 cagaagaaga gcattcaatt ccactggaag aatagcaacc aaatcaaaat acttggaaac
     7321 cagggaagct ttctgaccaa ggggccaagt aaactgaatg accgagctga ttcacgcagg
     7381 tcactttggg atcaaggcaa ctttcctttg atcattaaga acttgaagat cgaagactct
     7441 gacacatata tttgcgaagt cgaagaccag aaggaggagg tgcagctgtt ggtatttgga
     7501 ttgaccgcca actcagacac tcacctcctg caaggacagt ccttgactct gacactggag
     7561 tcccctcccg gttcatcccc ttctgtacag tgccgcagcc cacggggaaa gaacatccaa
     7621 ggagggaaga cactttccgt ttcccagctt gagcttcaag attctgggac ctggacttgt
     7681 accgtgctgc agaaccagaa gaaagtagaa ttcaagattg acatagttgt tctggcgttt
     7741 caaaaagctt cttctggact caacgacatc tttgaggcac agaagattga gtggcacgag
     7801 gactacaagg acgacgacga taaatgatag ctcgaggccg gcaaggccgg atccagacat
     7861 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7921 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     7981 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     8041 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc
     8101 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8161 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8221 ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatcccc gccccggacg
     8281 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     8341 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     8401 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     8461 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     8521 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     8581 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     8641 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     8701 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     8761 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     8821 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     8881 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     8941 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     9001 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     9061 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     9121 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     9181 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     9241 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     9301 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     9361 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     9421 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     9481 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     9541 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     9601 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     9661 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     9721 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     9781 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     9841 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     9901 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     9961 aggctatcct
//
