LOCUS       sequence-19314-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf237
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             462..503
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             513..530
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(553..570)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    559..669
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgagctcgg atccagtacc cttcaccgaa ttccagctga ccaccatggt aatatattgc
       61 agcaattctc taggtgcata ttgcaaactg gtcattaaca aagtcgcagt attgttaaca
      121 tatttctgtt ttgtggcact tactaatgca caattctttg tttcagatat cttagtccat
      181 ggcaagatac ctcccaacca tctaatcata caatatccaa gtgtttctag atctccacgt
      241 ctagatacaa cgtatccttt atgcgaatct ataggtgtaa attctagagt accgttatcc
      301 attttatttg gatttcttat aaatggaaca tgttctccat tagacatgaa tttagaacca
      361 accgtaagca attcccgggg atccgaataa agggtcaaga caattctgca gatatccagc
      421 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
      481 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa
      541 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
      601 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaatgagga
      661 aattgcatcg cattgtctga gtaggtgtca ttctatttct ggggggtggg gtggggcagg
      721 ac
//
