LOCUS       sequence-193148-       10568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-tagged human CCR5 fused to N-terminus of split Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-FLAG-CCR5-VN
FEATURES             Location/Qualifiers
     source          1..10568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(726..744)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        812..916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(917..985,986..1777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1135..1154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1948..2536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2437..2456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2967..3112
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3155..4192
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4190..4209)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4234..4282
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4936..5315
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5316..5519
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5470..5490
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5516..5540
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5808..5819
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             join(6714..6716,6717..7175)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6756..6777)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    7198..7332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7222..7241)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7276..7295
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7475..7497)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8057..9846
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccctcccgct cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact
       61 tggacgtttt tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc
      121 cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt
      181 atcaccccct cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc
      241 cttctctcct aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac
      301 taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac
      361 tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat
      421 ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca
      481 gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct
      541 caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct
      601 cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg
      661 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
      721 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      781 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1141 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1441 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     1501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2581 gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     2641 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     2701 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg
     2761 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     2821 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     2881 cgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa
     2941 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
     3001 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
     3061 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
     3121 acagcgtgcc gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     3181 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     3241 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     3301 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     3361 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     3421 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     3481 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     3541 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     3601 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     3661 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     3721 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3781 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3841 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3901 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     3961 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     4021 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     4081 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     4141 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga
     4201 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4261 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4321 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     4381 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     4441 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
     4501 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga
     4561 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
     4621 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
     4681 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     4741 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     4801 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     4861 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     4921 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4981 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     5041 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     5101 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     5161 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     5221 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     5281 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     5341 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     5401 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     5461 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     5521 gtttagtgaa ccgtcagatc tctagaagct gggtaccagc tgctagcgcc accatgaaga
     5581 ccatcatcgc cctgagctac atcttctgcc tggtgttcgc cgactacaag gacgatgacg
     5641 acggcagccc cggaggcgcg gattatcaag tgtcaagtcc aatctatgac atcaattatt
     5701 atacatcgga gccctgccaa aaaatcaatg tgaagcaaat cgcagcccgc ctcctgcctc
     5761 cgctctactc actggtgttc atctttggtt ttgtgggcaa catgctggtc atcctcatcc
     5821 tgataaactg caaaaggctg aagagcatga ctgacatcta cctgctcaac ctggccatct
     5881 ctgacctgtt tttccttctt actgtcccct tctgggctca ctatgctgcc gcccagtggg
     5941 actttggaaa tacaatgtgt caactcttga cagggctcta ttttataggc ttcttctctg
     6001 gaatcttctt catcatcctc ctgacaatcg ataggtacct ggctgtcgtc catgctgtgt
     6061 ttgctttaaa agccaggacg gtcacctttg gggtggtgac aagtgtgatc acttgggtgg
     6121 tggctgtgtt tgcgtctctc ccaggaatca tctttaccag atctcaaaaa gaaggtcttc
     6181 attacacctg cagctctcat tttccataca gtcagtatca attctggaag aatttccaga
     6241 cattaaagat agtcatcttg gggctggtcc tgccgctgct tgtcatggtc atctgctact
     6301 cgggaatcct aaaaactctg cttcggtgtc gaaatgagaa gaagaggcac agggctgtga
     6361 ggcttatctt caccatcatg attgtttatt ttctcttctg ggctccctac aacattgtcc
     6421 ttctcctgaa caccttccag gaattctttg gcctgaataa ttgcagtagc tctaacaggt
     6481 tggaccaagc tatgcaggtg acagagactc ttgggatgac gcactgctgc atcaacccca
     6541 tcatctatgc ctttgtcggg gagaagttca gaaactacct cttagtcttc ttccaaaagc
     6601 acattgccaa acgcttctgc aaatgctgtt ctattttcca gcaagaggct cccgagcgag
     6661 caagctcagt ttacacccga tccactgggg agcaggaaat atctgtgggc ttggtgagca
     6721 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     6781 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     6841 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     6901 ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     6961 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7021 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7081 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7141 acaactacaa cagccacaac gtctatctga ccgcctaact cgaggccggc aaggccggat
     7201 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7261 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7321 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     7381 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     7441 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7501 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7561 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     7621 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     7681 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     7741 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     7801 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     7861 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     7921 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     7981 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     8041 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     8101 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     8161 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     8221 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     8281 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     8341 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     8401 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     8461 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     8521 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     8581 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     8641 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     8701 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     8761 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     8821 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     8881 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     8941 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     9001 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     9061 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     9121 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     9181 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     9241 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     9301 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     9361 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     9421 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     9481 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9541 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9601 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     9661 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     9721 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     9781 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     9841 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     9901 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     9961 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
    10021 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
    10081 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
    10141 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
    10201 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
    10261 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
    10321 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
    10381 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
    10441 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
    10501 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
    10561 cgacccaa
//
