LOCUS       sequence-193150-       12119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-tagged human mGluR3 fused to N-terminus of split Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 12119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-FLAG-GRM3-VN
FEATURES             Location/Qualifiers
     source          1..12119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(708..726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        794..898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(899..967,968..1759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1117..1136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1930..2518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2419..2438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2949..3094
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3137..4174
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4172..4191)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4216..4264
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4918..5297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5298..5501
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5452..5472
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5498..5522
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5550..5559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5634..5657
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(8217..8219,8220..8678)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(8259..8280)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8520..8539)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    8701..8835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8725..8744)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8779..8798
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8978..9000)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9560..11349
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct
       61 ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc
      121 gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg
      181 aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt
      241 tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac
      301 atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag
      361 cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc
      421 catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct
      481 gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct
      541 ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac
      601 cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc
      661 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
      721 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      781 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      901 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      961 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1021 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1081 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1141 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1201 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1261 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1321 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1381 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1441 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1501 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1561 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1621 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1681 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1741 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1801 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1861 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1921 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1981 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2041 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2101 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2161 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2221 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2281 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2341 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2401 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2461 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2521 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg
     2581 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2641 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2701 gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac
     2761 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
     2821 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg
     2881 tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta
     2941 gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga
     3001 tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct
     3061 cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc
     3121 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     3181 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     3241 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     3301 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     3361 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     3421 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     3481 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     3541 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     3601 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     3661 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     3721 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     3781 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     3841 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     3901 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     3961 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     4021 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     4081 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     4141 tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     4201 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     4261 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     4321 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     4381 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc
     4441 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt
     4501 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg
     4561 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc
     4621 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac
     4681 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     4741 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     4801 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     4861 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
     4921 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4981 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     5041 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     5101 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     5161 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     5221 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     5281 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     5341 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     5401 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     5461 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     5521 tctctagaag ctgggtacca gctgctagcg ccaccatggc cgtcatggcg ccccgaaccc
     5581 tcgtcctgct actctcgggg gccctggccc tgacccagac ctgggcgggc tccgactaca
     5641 aggacgatga tgacaaggga ggatccagga gagagattaa aatagaaggt gaccttgttt
     5701 tagggggcct gtttcctatt aacgaaaaag gcactggaac tgaagaatgt gggcgaatca
     5761 atgaagaccg agggattcaa cgcctggaag ccatgttgtt tgctattgat gaaatcaaca
     5821 aagatgatta cttgctacca ggagtgaagt tgggtgttca cattttggat acatgttcaa
     5881 gggataccta tgcattggag caatcactgg agtttgtcag ggcatctttg acaaaagtgg
     5941 atgaagctga gtatatgtgt cctgatggat cctatgccat tcaagaaaac atcccacttc
     6001 tcattgcagg ggtcattggt ggctcttata gcagtgtttc catacaggtg gcaaacctgc
     6061 tgcggctctt ccagatccct cagatcagct acgcatccac cagcgccaaa ctcagtgata
     6121 agtcgcgcta tgattacttt gccaggaccg tgccccccga cttctaccag gccaaagcca
     6181 tggctgagat cttgcgcttc ttcaactgga cctacgtgtc cacagtagcc tccgagggtg
     6241 attacgggga gacagggatc gaggccttcg agcaggaagc ccgcctgcgc aacatctgca
     6301 tcgctacggc ggagaaggtg ggccgctcca acatccgcaa gtcctacgac agcgtgatcc
     6361 gagaactgtt gcagaagccc aacgcgcgcg tcgtggtcct cttcatgcgc agcgacgact
     6421 cgcgggagct cattgcagcc gccagccgcg ccaatgcctc cttcacctgg gtggccagcg
     6481 acggctgggg cgcgcaggag agcatcatca agggcagcga gcatgtggcc tacggcgcca
     6541 tcaccctgga gctggcctcc cagcctgtcc gccagttcga ccgctacttc cagagcctca
     6601 acccctacaa caaccaccgc aacccctggt tccgggactt ctgggagcaa aagtttcagt
     6661 gcagcctcca gaacaaacgc aaccacaggc gcgtctgcga caagcacctg gccatcgaca
     6721 gcagcaacta cgagcaagag tccaagatca tgtttgtggt gaacgcggtg tatgccatgg
     6781 cccacgcttt gcacaaaatg cagcgcaccc tctgtcccaa cactaccaag ctttgtgatg
     6841 ctatgaagat cctggatggg aagaagttgt acaaggatta cttgctgaaa atcaacttca
     6901 cggctccatt caacccaaat aaagatgcag atagcatagt caagtttgac acttttggag
     6961 atggaatggg gcgatacaac gtgttcaatt tccaaaatgt aggtggaaag tattcctact
     7021 tgaaagttgg tcactgggca gaaaccttat cgctagatgt caactctatc cactggtccc
     7081 ggaactcagt ccccacttcc cagtgcagcg acccctgtgc ccccaatgaa atgaagaata
     7141 tgcaaccagg ggatgtctgc tgctggattt gcatcccctg tgaaccctac gaatacctgg
     7201 ctgatgagtt tacctgtatg gattgtgggt ctggacagtg gcccactgca gacctaactg
     7261 gatgctatga ccttcctgag gactacatca ggtgggaaga cgcctgggcc attggcccag
     7321 tcaccattgc ctgtctgggt tttatgtgta catgcatggt tgtaactgtt tttatcaagc
     7381 acaacaacac acccttggtc aaagcatcgg gccgagaact ctgctacatc ttattgtttg
     7441 gggttggcct gtcatactgc atgacattct tcttcattgc caagccatca ccagtcatct
     7501 gtgcattgcg ccgactcggg ctggggagtt ccttcgctat ctgttactca gccctgctga
     7561 ccaagacaaa ctgcattgcc cgcatcttcg atggggtcaa gaatggcgct cagaggccaa
     7621 aattcatcag ccccagttct caggttttca tctgcctggg tctgatcctg gtgcaaattg
     7681 tgatggtgtc tgtgtggctc atcctggagg ccccaggcac caggaggtat acccttgcag
     7741 agaagcggga aacagtcatc ctaaaatgca atgtcaaaga ttccagcatg ttgatctctc
     7801 ttacctacga tgtgatcctg gtgatcttat gcactgtgta cgccttcaaa acgcggaagt
     7861 gcccagaaaa tttcaacgaa gctaagttca taggttttac catgtacacc acgtgcatca
     7921 tctggttggc cttcctccct atattttatg tgacatcaag tgactacaga gtgcagacga
     7981 caaccatgtg catctctgtc agcctgagtg gctttgtggt cttgggctgt ttgtttgcac
     8041 ccaaggttca catcatcctg tttcaacccc agaagaatgt tgtcacacac agactgcacc
     8101 tcaacaggtt cagtgtcagt ggaactggga ccacatactc tcagtcctct gcaagcacgt
     8161 atgtgccaac ggtgtgcaat gggcgggaag tcctcgactc caccacctca tctctggtga
     8221 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     8281 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     8341 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     8401 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     8461 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     8521 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     8581 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     8641 agtacaacta caacagccac aacgtctatc tgaccgccta actcgaggcc ggcaaggccg
     8701 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     8761 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     8821 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     8881 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     8941 ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     9001 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9061 cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc gactctagag gatcgatccc
     9121 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     9181 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     9241 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     9301 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     9361 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     9421 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     9481 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     9541 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     9601 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     9661 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     9721 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     9781 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     9841 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     9901 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     9961 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
    10021 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
    10081 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
    10141 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
    10201 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
    10261 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
    10321 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
    10381 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
    10441 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
    10501 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
    10561 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
    10621 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
    10681 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
    10741 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
    10801 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
    10861 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
    10921 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
    10981 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    11041 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    11101 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
    11161 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
    11221 gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg
    11281 agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc
    11341 cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc
    11401 gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg
    11461 agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac
    11521 gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg
    11581 ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta
    11641 acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt
    11701 catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca
    11761 aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct
    11821 ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta
    11881 aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc
    11941 caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa
    12001 gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac
    12061 cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagct
//
