LOCUS       sequence-193152-        6158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with MX1 enhancer inserted downstream 
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_MX1
FEATURES             Location/Qualifiers
     source          1..6158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    670..791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(707..726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     761..780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(835..2487)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      2484..2493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      2592..3180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3081..3100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3370..4161,4162..4230))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4388..4405
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccttcatct ctgtctgggg cccaccagag agccctgcgg agaggagcag aagctgaaat
       61 cctttgatac tggtgagctt tggagagcca agctgggggc tgcaggacct gggttcaggc
      121 attgtgtaca ggtggcccag ccactgaagg aaagcaaaat attttacccc taaaatcttt
      181 tttttttttt ttttttttga gatggagtct cactcttgtc atccaggctg gagtgcagtg
      241 gggcaatctc ggcttactgc aacatttacc tcctgggttc aagcgattct cctgcctcag
      301 cctcctgagt agctgggact acaggcatgc gccaccaagc ccggctagtt tttgtatttt
      361 tagtagatac aaggtctcac catgttggcc aggctggtct tgaactcctg acttcaggtg
      421 atccacacgc ctcggcctcc taaagtgctg ggattacagg catgagccac cgtgcccggc
      481 ccaaaatcta tttctttagt catgccccgc gcccaccgga aggagctgac tgggttgaag
      541 gctctcaagg gcatcggtcg acggatcctt atcgatttta ccacatttgt agaggtttta
      601 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      661 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      721 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      781 aatgtatctt atcatgtctg ctcgaagcgg ccggccgccc cgactctaga attattacac
      841 ggcgatcttg ccgcccttct tggccttaat gagaatctcg cggatcttgc gggcgtccaa
      901 cttgccggtc agtcctttag gcacctcgtc cacgaacaca acaccaccgc gcagcttctt
      961 ggcggttgta acctggctgg ccacatagtc cacgatctcc ttctcggtca tggttttacc
     1021 gtgttccagc acgacgactg cggcgggcag ctcgccggca tcgtcgtcgg gcaggccggc
     1081 gaccccggcg tcgaagatgt tggggtgttg cagcaggatg ctctccagtt cggctggggc
     1141 tacctggtag cccttgtatt tgatcaggct cttcagccgg tccacgatga agaagtgctc
     1201 gtcctcgtcc cagtaggcga tgtcgccgct gtgcagccag ccgtccttgt cgatgagagc
     1261 gtttgtagcc tcggggttgt taacgtagcc gctcatgatc atggggccac ggacgcacag
     1321 ctcgccgcgc tggttcacac ccagtgtctt accggtgtcc aagtccacca ccttagcctc
     1381 gaagaagggc accaccttgc ctactgcgcc aggcttgtcg tccccttcgg gggtgatcag
     1441 aatggcgctg gttgtttctg tcaggccgta gccctggcgg atgcctggta ggtggaagcg
     1501 tttggccacg gcctcaccta cctccttgct gagcggcgcc ccgccgctgg cgatctcgtg
     1561 caagttgctt aggtcgtact tgtcgatgag agtgctctta gcgaagaagc taaatagtgt
     1621 gggcaccagc agggcagatt gaatcttata gtcttgcaag ctgcgcaaga atagctcctc
     1681 ctcgaagcgg tacatgagca cgacccgaaa gccgcagatc aagtagccca gcgtggtgaa
     1741 catgccgaag ccgtggtgaa atggcaccac gctgaggata gcggtgtcgg ggatgatctg
     1801 gttgccgaag atggggtcgc gggcatgact gaatcggaca caagcggtgc ggtgcggtag
     1861 ggctacgccc ttgggcaatc cggtactgcc actactgttc atgatcaggg cgatggtttt
     1921 gtcccggtcg aagctctcgg gcacgaagtc gtactcgttg aagccgggtg gcaaatggga
     1981 agtcacgaag gtgtacatgc tttggaagcc ctggtagtcg gtcttgctat ccatgatgat
     2041 gatcttttgt atgatcggta gcttcttttg cacgttgagg atcttttgca gccctttctt
     2101 gctcacgaat acgacggtgg gctggctgat gcccatgctg ttcagcagct cgcgctcgtt
     2161 gtagatgtcg ttagctgggg ccacagccac accgatgaac agggcaccca acacgggcat
     2221 gaagaactgc aagctattct cgctgcacac cacgatccga tggtttgtat tcagcccata
     2281 gcgcttcata gcttctgcca gccgaacgct catctcgaag tactcggcgt aggtaatgtc
     2341 cacctcgata tgtgcgtcgg taaaggcgat ggtgccgggc accagggcgt agcgcttcat
     2401 ggctttgtgc agctgctcgc cggcggtccc gtcttcgagt gggtagaatg gcgctgggcc
     2461 cttcttaatg tttttggcat cttccatggt ggctttacca acagtaccgg attgccaagc
     2521 ttcaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2581 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2641 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2701 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2761 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2821 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2881 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2941 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3001 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3061 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3121 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3181 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3241 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3301 aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca
     3361 ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg
     3421 ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc
     3481 atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc
     3541 agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc
     3601 ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag
     3661 tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat
     3721 ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg
     3781 aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt
     3841 gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag
     3901 atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg
     3961 accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt
     4021 gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct
     4081 attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac
     4141 tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat
     4201 gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat
     4261 ttatcagggt tactagtacg tctctcaaga catgttcttt cctgcgttat cccctgattc
     4321 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4381 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc tgccggcacc tgtcctacga
     4441 gttgcatgat aaagaagaca gtcataagtg cggcgacgaa atggattaag ggcggtgcaa
     4501 gatgtgcttt gttaaacaga tgcttgaagg cagcatgctc cttaagagtc atcaccacgc
     4561 cctaatctca agtacccagg gacacaaaca ctgcggaagg ccgcagggac ctctgcctag
     4621 gaaagccagg tattgtccaa ggtttctccc catgtgatag tctgaaatac ggcctcgtgg
     4681 gaagggaaag acctgaccgt cccccagtcc gacacccata aagggtctgt gctgaggagg
     4741 attaatataa gaggaaggca tgcctcttgc agttgagaca agaggaaggc atctgtctcc
     4801 tgcccgtccc tgggcaatgg aatgtctcgg tataaaaccc gattgtacgt tccatctact
     4861 gagataggga aaaaccactt taaggctgga ggtgggacat gcaggcagca atactgcttt
     4921 gtaaagcatt gagacgttta tgtgtatgca tatctaaaag cacagcactt gattctttac
     4981 cctgtctatg atgcaaagac ctttgttcac gtgtttgtct gctgactctc tccccactat
     5041 tgtcttgtga ccctgacaca tccccctctc ggagaaacac ccacaaatga tcaataaata
     5101 ctaagggaac tcagaggctg gcgggatcct ccatatgctg aacgctggtt ccctgggtcc
     5161 ccttatttct ttctctatac tttgtctctg tgtctttttc ttttccaagt ctctccttcc
     5221 acctaacgag aaacacccac aggtgtggag gggcaaccca ccccttcagg tgcctgacac
     5281 cacaggctcc tgggcgggca gggcccagtg gagcctgacc ttgtggcact ggcctggcct
     5341 ctgctaccag gctgaggacc gggtcaaccg gcaaccccag gcccacgctg ggtgccgctg
     5401 cacttgggat ccgggcacag cgtggttcgg tgcttctgga atgaatgggg aatcggaagg
     5461 ccggccctgg tcctgcaaac acgggcctca ggatcctgca ctgggcttac ttctccctgc
     5521 gctctccgcc cccaccgcga atttcccgga caattcagtt tcggtttcat ttctgctcgc
     5581 tggtttccag aaaggaaaag tcaaattgca tgacttcaga cttcagaatc cagggggcgg
     5641 ggaagggggc agggaagaag ggggcggtgc cacgacgagg gggcagtgtt actcacatac
     5701 accgctagca cctcccccag cctggacccg gttctgctgg gccggggagg cgggtggtcg
     5761 cggaacggcg gagcatccca caacctgccc atcctcccgg ctgcatttcc tgcaagtccg
     5821 tccgcctgcc cgcgcccgcg cccgcgcccc cgcccccacc cttgcccgct ttgtcctgcc
     5881 tgggcgcgag taaagggact ggtttccttt gcctcgtttc gtttcctctt ctgtgcgtgt
     5941 ggtatagacc cgtgcttctc aaagggtggt tcctggaaca gcagcattgg cttcacctgg
     6001 gaaattatta gaaatgaacg gtccggggcc atcccaagac ccctgcatta ccacctgtat
     6061 tttaagggcc ttcctcaggt gatcccttgg caactgggca cgagactgct ggcgtctgac
     6121 gtggtcgctt ttggtcctca aggtcaaggc cagacttt
//
