LOCUS       sequence-193153-       10706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  myc-tagged human CCR5 fused to C-terminus of split Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-myc-CCR5-VC
FEATURES             Location/Qualifiers
     source          1..10706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(715..733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        801..905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(906..974,975..1766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1124..1143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2956..3101
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3144..4181
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4179..4198)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4223..4271
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4925..5304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5305..5508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5459..5479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5505..5529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5611..5640
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    5806..5817
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             6712..6963
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     6900..6921
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6986..7120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7010..7029)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7064..7083
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7263..7285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7845..9634
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
       61 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
      121 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
      181 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
      241 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
      301 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
      361 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
      421 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
      481 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
      541 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
      601 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
      661 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     2581 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     2641 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     2701 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     2761 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     2821 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     2881 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     2941 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     3001 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     3061 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     3121 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     3181 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     3241 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3301 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     3361 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     3421 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     3481 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     3541 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     3601 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     3661 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3721 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     3781 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     3841 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     3901 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     3961 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     4021 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4081 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     4141 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
     4201 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     4261 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4321 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4381 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
     4441 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     4501 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
     4561 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
     4621 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
     4681 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     4741 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     4801 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     4861 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     4921 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4981 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5041 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5101 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5161 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     5221 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     5281 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     5341 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     5401 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     5461 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     5521 cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgaagac catcatcgcc
     5581 ctgagctaca tcttctgcct ggtgttcgcc gagcaaaagc tgatctcaga ggaggacctg
     5641 ggcagccctg gcggtgccga ttatcaagtg tcaagtccaa tctatgacat caattattat
     5701 acatcggagc cctgccaaaa aatcaatgtg aagcaaatcg cagcccgcct cctgcctccg
     5761 ctctactcac tggtgttcat ctttggtttt gtgggcaaca tgctggtcat cctcatcctg
     5821 ataaactgca aaaggctgaa gagcatgact gacatctacc tgctcaacct ggccatctct
     5881 gacctgtttt tccttcttac tgtccccttc tgggctcact atgctgccgc ccagtgggac
     5941 tttggaaata caatgtgtca actcttgaca gggctctatt ttataggctt cttctctgga
     6001 atcttcttca tcatcctcct gacaatcgat aggtacctgg ctgtcgtcca tgctgtgttt
     6061 gctttaaaag ccaggacggt cacctttggg gtggtgacaa gtgtgatcac ttgggtggtg
     6121 gctgtgtttg cgtctctccc aggaatcatc tttaccagat ctcaaaaaga aggtcttcat
     6181 tacacctgca gctctcattt tccatacagt cagtatcaat tctggaagaa tttccagaca
     6241 ttaaagatag tcatcttggg gctggtcctg ccgctgcttg taatggtcat ctgctactcg
     6301 ggaatcctaa aaactctgct tcggtgtcga aatgagaaga agaggcacag ggctgtgagg
     6361 cttatcttca ccatcatgat tgtttatttt ctcttctggg ctccctacaa cattgtcctt
     6421 ctcctgaaca ccttccagga attctttggc ctgaataatt gcagtagctc taacaggttg
     6481 gaccaagcta tgcaggtgac agagactctt gggatgacgc actgctgcat caaccccatc
     6541 atctatgcct ttgtcgggga gaagttcaga aactacctct tagtcttctt ccaaaagcac
     6601 attgccaaac gcttctgcaa atgctgttct attttccagc aagaggctcc cgagcgagca
     6661 agctcagttt acacccgatc cactggggag caggaaatat ctgtgggctt ggacaagcag
     6721 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     6781 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     6841 aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     6901 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     6961 aagtaactcg aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg
     7021 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     7081 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     7141 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     7201 caaatgtggt atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa
     7261 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7321 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7381 aggtcgactc tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc
     7441 tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa
     7501 ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg
     7561 tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg
     7621 gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc
     7681 tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg
     7741 gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag
     7801 agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc
     7861 ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca
     7921 acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc
     7981 tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt
     8041 gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct
     8101 gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt
     8161 gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata
     8221 aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa
     8281 accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag
     8341 aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg
     8401 ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag
     8461 gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga
     8521 gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg
     8581 ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg
     8641 tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa
     8701 gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca
     8761 caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag
     8821 gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca
     8881 cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca
     8941 tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg
     9001 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     9061 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     9121 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     9181 tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg
     9241 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     9301 atatgctatc ctaatctata tctgggtagy atakgctatc ctaatctata tctgggtagc
     9361 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     9421 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     9481 atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata tacagtcagc
     9541 atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc acctcccaag
     9601 ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt gctcccattc ttaggtgaat
     9661 ttaaggaggc caggctaaag ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa
     9721 tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa catgggtatg
     9781 cccaattgcc ccatgttggg aggacgaaaa tggtgacaag acagatggcc agaaatacac
     9841 caacagcacg catgatgtct actggggatt tattctttag tgcgggggaa tacacggctt
     9901 ttaatacgat tgagggcgtc tcctaacaag ttacatcact cctgcccttc ctcaccctca
     9961 tctccatcac ctccttcatc tccgtcatct ccgtcatcac cctccgcggc agccccttcc
    10021 accataggtg gaaaccaggg aggcaaatct actccatcgt caaagctgca cacagtcacc
    10081 ctgatattgc aggtaggagc gggctttgtc ataacaaggt ccttaatcgc atccttcaaa
    10141 acctcagcaa atatatgagt ttgtaaaaag accatgaaat aacagacaat ggactccctt
    10201 agcgggccag gttgtgggcc gggtccaggg gccattccaa aggggagacg actcaatggt
    10261 gtaagacgac attgtggaat agcaagggca gttcctcgcc ttaggttgta aagggaggtc
    10321 ttactacctc catatacgaa cacaccggcg acccaagttc cttcgtcggt agtcctttct
    10381 acgtgactcc tagccaggag agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg
    10441 gaacctcctt gaccacgatg ctttccaaac caccctcctt ttttgcgcct gcctccatca
    10501 ccctgacccc ggggtccagt gcttgggcct tctcctgggt catctgcggg gccctgctct
    10561 atcgctcccg ggggcacgtc aggctcacca tctgggccac cttcttggtg gtattcaaaa
    10621 taatcggctt cccctacagg gtggaaaaat ggccttctac ctggaggggg cctgcgcggt
    10681 ggagacccgg atgatgatga ctgact
//
