LOCUS       sequence-19316-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf239
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             421..462
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             472..489
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(512..529)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    518..629
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cggatccagt acccttcacc gaattccagc tgaccaccat gcgtatgcac ataacagcat
       61 tctttaccat tcacaatatc aaaatgatca tatacgacag accacatatg gcgtatagtt
      121 tccaaacgtt tcatgagtac attattcacg ctgtcttcca gagaggtggt tacctcgatg
      181 gtgccgccga tatagtagaa gaaagatttt ctgtatcatt ctccccaagt ttaactttta
      241 ccagatttgg gatcggaagc accgcaccct ttttgaatct cattctcata atttcctttc
      301 catgtacgtc cacagcagta acttgagcaa ttcccgggga tccgaataaa gggtcaagac
      361 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
      421 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac
      481 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      541 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      601 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattct
//
