LOCUS       sequence-193170-        5959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with OAS3 enhancer inserted downstream 
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_OAS3
FEATURES             Location/Qualifiers
     source          1..5959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1956..2077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1993..2012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2047..2066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(2121..3773)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      3770..3779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      3878..4466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4367..4386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4656..5447,5448..5516))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5674..5691
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtgactcaga gtagtaattt agcgtctagc ctcagtttcc ctatccataa aacaggtaca
       61 ataatacctg cctcactgaa cgtggggatg aaatgaggta atactcattt aatgagataa
      121 ttttcattgt tatttgataa tgtctcaata aattgggcta ctctggctgt tacgcggagt
      181 gttattactt ctgtatcctt aaggtctggc atagtgtgag gcccacagtc tgtttgaatg
      241 actgggagaa tgagtgaatg agtgaatgaa tggataagaa gcagcctctg agagtagaga
      301 tgcaggaatc aggctacttg gatttgaatc ctggctgggc ctcttacagg atcggacacg
      361 tcactgtacc tctctgtgcc tcattttctc tatctgtcaa gcgggcttaa gagtacctac
      421 ctggctcgta tgttcggggg agttagcacg gggctggcac attggaagta tttgttaaag
      481 aaatgaatga ggtgcacaag cggataaagc cacccccacc gtaagcctat gtgaggctta
      541 agcgtcctcc caggactccc ctttgccctt tggacacata aagaccccga ggtgggacct
      601 caccttgaca gttttcagca cccgcggggc agcagcaccg aggcggcccc cgcgctccct
      661 cagagcagcg gccagggcgc ccagagcgcg ccgcgccttc tctacgaact ccttccgcgg
      721 ctgcagcttt ctggccacga acctgtccag cgcagcggcc ggggtgctgt acaagtccat
      781 ggcctcccgg cgcagatgca aggggaagca gggctctggc gcggccttcg gatttctggt
      841 ttcgttttcc cgcccggctc tccgaaccac gcccagctgg ccaggtccta ggtcctcggt
      901 cccgcgcgtg gcgtgaaacg gggaggggcg ctgcagttcc tgtcttcccc aaacttggga
      961 aattccctgc tttcagtttc gtttctttct agcagctctt ggacctgaca cccacttcct
     1021 ggtccccttc ccagctacga cttcctctcc ttcatctcct tgcttccagc cgtccctgca
     1081 accaccagct ccaacgaact aatcacaacc taacaaagta tacccacaga acgtatgggt
     1141 acatactgag taacgtactc tgagcatttc agaaacgtgc aggttttaca tggaaaaaaa
     1201 aggtcaggat ttaataaacg gagaagtcct acctcgctgt ctgactgagg gatgcgttct
     1261 aaacaccact accaccacca acgataataa agcacacttg gagactctca ctgactcctc
     1321 ccaacgacca tggtgtaggt gctattatca tcacccattt gacagatgag gaagtcaagg
     1381 tacagaggaa ctaggtaact gtccaaggta acacagctgg aaagttgtct ggctggattc
     1441 aaacccagcc caagcccaca gagctgccct cttcagaagc caactcttcc taaataattt
     1501 atacgtgtaa tgcagttcca ataaaaattc catctttaaa aacttcaaac acacgatcta
     1561 aagtctgtct gcaagaatta aaaattattt tggaggagaa ggaggaagga aagtcagtca
     1621 gtttaggatg gtaccttgca caacaatact acatattaaa acattgtaaa gctacaatag
     1681 ggaaatcctc ttggaatggg cacaaagcta agcggactgt aagctataag ctgcagagtg
     1741 gagatttgaa tccaggacca ttgcgcttct atcttcatgg cctagtcatg ccccgcgccc
     1801 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgg atccttatcg
     1861 attttaccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     1921 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     1981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgctcg aagcggccgg
     2101 ccgccccgac tctagaatta ttacacggcg atcttgccgc ccttcttggc cttaatgaga
     2161 atctcgcgga tcttgcgggc gtccaacttg ccggtcagtc ctttaggcac ctcgtccacg
     2221 aacacaacac caccgcgcag cttcttggcg gttgtaacct ggctggccac atagtccacg
     2281 atctccttct cggtcatggt tttaccgtgt tccagcacga cgactgcggc gggcagctcg
     2341 ccggcatcgt cgtcgggcag gccggcgacc ccggcgtcga agatgttggg gtgttgcagc
     2401 aggatgctct ccagttcggc tggggctacc tggtagccct tgtatttgat caggctcttc
     2461 agccggtcca cgatgaagaa gtgctcgtcc tcgtcccagt aggcgatgtc gccgctgtgc
     2521 agccagccgt ccttgtcgat gagagcgttt gtagcctcgg ggttgttaac gtagccgctc
     2581 atgatcatgg ggccacggac gcacagctcg ccgcgctggt tcacacccag tgtcttaccg
     2641 gtgtccaagt ccaccacctt agcctcgaag aagggcacca ccttgcctac tgcgccaggc
     2701 ttgtcgtccc cttcgggggt gatcagaatg gcgctggttg tttctgtcag gccgtagccc
     2761 tggcggatgc ctggtaggtg gaagcgtttg gccacggcct cacctacctc cttgctgagc
     2821 ggcgccccgc cgctggcgat ctcgtgcaag ttgcttaggt cgtacttgtc gatgagagtg
     2881 ctcttagcga agaagctaaa tagtgtgggc accagcaggg cagattgaat cttatagtct
     2941 tgcaagctgc gcaagaatag ctcctcctcg aagcggtaca tgagcacgac ccgaaagccg
     3001 cagatcaagt agcccagcgt ggtgaacatg ccgaagccgt ggtgaaatgg caccacgctg
     3061 aggatagcgg tgtcggggat gatctggttg ccgaagatgg ggtcgcgggc atgactgaat
     3121 cggacacaag cggtgcggtg cggtagggct acgcccttgg gcaatccggt actgccacta
     3181 ctgttcatga tcagggcgat ggttttgtcc cggtcgaagc tctcgggcac gaagtcgtac
     3241 tcgttgaagc cgggtggcaa atgggaagtc acgaaggtgt acatgctttg gaagccctgg
     3301 tagtcggtct tgctatccat gatgatgatc ttttgtatga tcggtagctt cttttgcacg
     3361 ttgaggatct tttgcagccc tttcttgctc acgaatacga cggtgggctg gctgatgccc
     3421 atgctgttca gcagctcgcg ctcgttgtag atgtcgttag ctggggccac agccacaccg
     3481 atgaacaggg cacccaacac gggcatgaag aactgcaagc tattctcgct gcacaccacg
     3541 atccgatggt ttgtattcag cccatagcgc ttcatagctt ctgccagccg aacgctcatc
     3601 tcgaagtact cggcgtaggt aatgtccacc tcgatatgtg cgtcggtaaa ggcgatggtg
     3661 ccgggcacca gggcgtagcg cttcatggct ttgtgcagct gctcgccggc ggtcccgtct
     3721 tcgagtgggt agaatggcgc tgggcccttc ttaatgtttt tggcatcttc catggtggct
     3781 ttaccaacag taccggattg ccaagcttca aaatccctta acgtgagttt tcgttccact
     3841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4021 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4081 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4141 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4201 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4261 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4321 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4381 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4441 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4501 ccttttgctg gccttttgct cacatgtcat gagattatca aaaaggatct tcacctagat
     4561 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4621 tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca
     4681 ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag
     4741 atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag
     4801 ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga
     4861 agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct
     4921 agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc
     4981 gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc
     5041 cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc
     5101 gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat
     5161 tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag
     5221 tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac
     5281 aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg
     5341 cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca
     5401 cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc
     5461 aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc
     5521 gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc tcaagacatg
     5581 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5641 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5701 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     5761 gacgactggg caacacggca aaactctatc tctacaaaaa atgcaaataa ttagccaggc
     5821 atggtagtgt gcacctgtag tgccagctac ttgggaggct gaggtgtgag aataacttga
     5881 gcccaggagg cggaggttgc tgtgagccaa gatcatgcta ctgcactcca gcctgggtga
     5941 cagagcgaga ccctgtctc
//
