LOCUS       sequence-193174-        5345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative control for luciferase validation vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase validation vector_ORI_neg...
FEATURES             Location/Qualifiers
     source          1..5345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    490..611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(527..546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     581..600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(655..2307)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      2304..2313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      2412..3000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2901..2920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3190..3981,3982..4050))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4208..4225
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttttacagct gtctgagggc ctatggcagt gtggcctctc agcaagacac tgctgaagtg
       61 gggctcaaac atttggcagt caagagaggg acaaaacctt tgtataattt gggagtgagt
      121 tcaaagccaa taacatttac actccaggcc aagaaagcat tttaaagcca gcaaggtgcc
      181 tctaagccaa taaggtgtta ttacctttaa gcacatctct tctgcccttt gataataagc
      241 gttgttttaa agtatttgaa aataccattc acaatttaga acaggatata tcacctccac
      301 actttgaaca cccaaatagt catgccccgc gcccaccgga aggagctgac tgggttgaag
      361 gctctcaagg gcatcggtcg acggatcctt atcgatttta ccacatttgt agaggtttta
      421 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      481 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      601 aatgtatctt atcatgtctg ctcgaagcgg ccggccgccc cgactctaga attattacac
      661 ggcgatcttg ccgcccttct tggccttaat gagaatctcg cggatcttgc gggcgtccaa
      721 cttgccggtc agtcctttag gcacctcgtc cacgaacaca acaccaccgc gcagcttctt
      781 ggcggttgta acctggctgg ccacatagtc cacgatctcc ttctcggtca tggttttacc
      841 gtgttccagc acgacgactg cggcgggcag ctcgccggca tcgtcgtcgg gcaggccggc
      901 gaccccggcg tcgaagatgt tggggtgttg cagcaggatg ctctccagtt cggctggggc
      961 tacctggtag cccttgtatt tgatcaggct cttcagccgg tccacgatga agaagtgctc
     1021 gtcctcgtcc cagtaggcga tgtcgccgct gtgcagccag ccgtccttgt cgatgagagc
     1081 gtttgtagcc tcggggttgt taacgtagcc gctcatgatc atggggccac ggacgcacag
     1141 ctcgccgcgc tggttcacac ccagtgtctt accggtgtcc aagtccacca ccttagcctc
     1201 gaagaagggc accaccttgc ctactgcgcc aggcttgtcg tccccttcgg gggtgatcag
     1261 aatggcgctg gttgtttctg tcaggccgta gccctggcgg atgcctggta ggtggaagcg
     1321 tttggccacg gcctcaccta cctccttgct gagcggcgcc ccgccgctgg cgatctcgtg
     1381 caagttgctt aggtcgtact tgtcgatgag agtgctctta gcgaagaagc taaatagtgt
     1441 gggcaccagc agggcagatt gaatcttata gtcttgcaag ctgcgcaaga atagctcctc
     1501 ctcgaagcgg tacatgagca cgacccgaaa gccgcagatc aagtagccca gcgtggtgaa
     1561 catgccgaag ccgtggtgaa atggcaccac gctgaggata gcggtgtcgg ggatgatctg
     1621 gttgccgaag atggggtcgc gggcatgact gaatcggaca caagcggtgc ggtgcggtag
     1681 ggctacgccc ttgggcaatc cggtactgcc actactgttc atgatcaggg cgatggtttt
     1741 gtcccggtcg aagctctcgg gcacgaagtc gtactcgttg aagccgggtg gcaaatggga
     1801 agtcacgaag gtgtacatgc tttggaagcc ctggtagtcg gtcttgctat ccatgatgat
     1861 gatcttttgt atgatcggta gcttcttttg cacgttgagg atcttttgca gccctttctt
     1921 gctcacgaat acgacggtgg gctggctgat gcccatgctg ttcagcagct cgcgctcgtt
     1981 gtagatgtcg ttagctgggg ccacagccac accgatgaac agggcaccca acacgggcat
     2041 gaagaactgc aagctattct cgctgcacac cacgatccga tggtttgtat tcagcccata
     2101 gcgcttcata gcttctgcca gccgaacgct catctcgaag tactcggcgt aggtaatgtc
     2161 cacctcgata tgtgcgtcgg taaaggcgat ggtgccgggc accagggcgt agcgcttcat
     2221 ggctttgtgc agctgctcgc cggcggtccc gtcttcgagt gggtagaatg gcgctgggcc
     2281 cttcttaatg tttttggcat cttccatggt ggctttacca acagtaccgg attgccaagc
     2341 ttcaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2521 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3121 aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca
     3181 ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg
     3241 ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc
     3301 atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc
     3361 agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc
     3421 ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag
     3481 tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat
     3541 ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg
     3601 aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt
     3661 gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag
     3721 atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg
     3781 accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt
     3841 gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct
     3901 attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac
     3961 tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat
     4021 gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat
     4081 ttatcagggt tactagtacg tctctcaaga catgttcttt cctgcgttat cccctgattc
     4141 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4201 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc tgccggcacc tgtcctacga
     4261 gttgcatgat aaagaagaca gtcataagtg cggcgacgac tggaaatgtg cgtggcagaa
     4321 gtttccctct ccttctagtt ttcctggtga atggaaatgc accagtgtgt cccatttcct
     4381 gctgggacat ggggcctttt tgtttctttg gcctcctggt gttgaaggta aactcccctg
     4441 gggagtactt gcagcttcat tgcatggtcc agcaaacttt agtttgtgac tatttcagct
     4501 ttaggtgaag tctgtcaagc aatggaggtg gattggcctt ccctgggtct tataaagaga
     4561 attggtttct agaatttggt tttttagagt atggacaagc agatccccat tactctcccc
     4621 atcacccccg aggaagtcta tgagaggaac ccttgaatgc tgatctatgt gtgacagctg
     4681 gggaaattga ggcatcaaag gaggagagaa ttccacatgc atcaaaatta aagaactaga
     4741 gagaaaacca tgacatccag gttgcctggt catgctttaa ccacaaaccg cactcgccta
     4801 tttagttaca acaaacacaa aagagataaa catagatgta agttagtcta aacaccaaat
     4861 tttagaagtt tttcctctga tgtctagtca gtcatcctga cctttattgg aggataaacc
     4921 aacttgcaac agctgtggat aattatttac tgtaagtcag agaccaaagg gcttggataa
     4981 tgaagtgaat tcggcattag aaggagagag atgtgctgta gcaccagaaa gttacctttt
     5041 aagttgattc ctgaatttca atgatcaaga ggtaccaaat tcactatcta agagatgctg
     5101 gaaaagcgca ggatgtgctg agcagtggac tttgttcaca tgtgaggggt tttacaaaaa
     5161 tgtacagcca cacctggccc ttcctcagcc tggaatcatc caagaagata ctttgtgcag
     5221 atgctactga gatgacacat cccttgctgt ccataaaagt gatctacagc tggctgtggt
     5281 gttacccagc catcttggct actcaataag aaacagattt ggattccctc actcaggctg
     5341 tcaga
//
