LOCUS       sequence-193199-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SaBE4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, 
            Kim YB, Badran AH, Liu DR
  TITLE     Improved base excision repair inhibition and bacteriophage Mu Gam 
            protein yields C:G-to-T:A base editors with higher efficiency and 
            product purity.
  JOURNAL   Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. 
            eCollection 2017 Aug.
  PUBMED    28875174
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2017-08-30"; volume: "3(8):eaao4774. doi"; pages: " 
            10.1126/sciadv.aao4774. eCollection 2017 Aug"
COMMENT     Sequence Label: SaBE4
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..39)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(52..300)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(331..579)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(610..675)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggtgatgatg accggttaga ctttcctctt cttcttggga gaaccaccag agagcatctt
       61 aatcttgttc tcaccgttgc tatcctgtat gaccagagcc caaggcttgt attcaggggc
      121 gtcgctagtc agaagcatga cattctcgtc ggtgctctcg tcgtaggcgg tgtgcacgag
      181 tatatcgctt tccggcttgt tcccaatgac ttcttccacc tcctctggga gcatgaggat
      241 ggattcctgg ataaccagtt gcttaccggt ctccttttca ataatatctg acagattagt
      301 agaaccacca gatcctccag aaccaccaga gagcatctta atcttgttct caccgttgct
      361 atcctgtatg accagagccc aaggcttgta ttcaggggcg tcgctagtca gaagcatgac
      421 attctcgtcg gtgctctcgt cgtaggcggt gtgcacgagt atatcgcttt ccggcttgtt
      481 cccaatgact tcttccacct cctctgggag catgaggatg gattcctgga taaccagttg
      541 cttaccggtc tccttttcaa taatatctga cagattagta gaaccaccag atcctccaga
      601 accaccagac ttgtcatcgt catccttgta atcgatgtca tgatctttat aatcaccgtc
      661 atggtctttg tagtcgctcg agacttt
//
