LOCUS       sequence-19322-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf250
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             590..631
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             641..658
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tacccttcac cgaattccag ctgaccacca tggcgactaa attagattat gaggatgctg
       61 ttttttactt tgtggatgat gataaaatat gtagtcgcga ctccatcatc gatctaatag
      121 atgaatatat tacgtggaga aatcatgtta tagtgtttaa caaagatatt accagttgtg
      181 gaagactgta caaggaattg atgaagttcg atgatgtcgc tatacggtac tatggtattg
      241 ataaagttaa tgagattgtc gaagctatga gcgaaggaga ccactacatc aattttacaa
      301 aagtccatga tcaggaaagt ttattcgcta ccataggaat atgtgctaaa atcactgaac
      361 attggggata caaaaagatt tcagaatcta gattccaatc attgggaaac attacagatc
      421 tgatgaccga cgataatata aacatcttga tactttttct agaaaaaaat tgaattgatg
      481 atataggggt cttcagcaat tcccggggat ccgaataaag ggtcaagaca attctgcaga
      541 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
      601 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
      661 agttttaacc cgctgatcag cctcgactgt gctttctagt tgccagccat ctgttgtttg
      721 cc
//
