LOCUS       sequence-193223-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetic depletion of FTCD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pLentiCRISPR sgFTCD_1
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             509..553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        731..979
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     731..751
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     902..921
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1001..1076
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aannnnaant agtaaaaatt gaancatnnn agtagcnccc nccaaggcaa nnngaagagn
       61 gtgcngngag aaaaagagca gtgggaatag nagctttgtt ccttngggtt cttgggagca
      121 gcaggaagcn ctatgggcgc agcgtcaatg acgctgacgg tacaggccca gacaattatt
      181 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
      241 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
      301 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
      361 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
      421 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
      481 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
      541 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
      601 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
      661 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
      721 gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
      781 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
      841 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      901 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      961 gtggaaagga cgaaacaccg gcaggttgag cgcgatgcgg gttttagagc tagaaatagc
     1021 aagttaaaat aaggatagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgc
//
