LOCUS       sequence-193224-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetic depletion of FTCD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pLentiCRISPR sgFTCD_2
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    79..312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             497..541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        719..967
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     719..739
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     890..909
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        989..1064
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaaaattgnn ccattagagt agnnccnnnn nnnncaannn nannnnnggn ngcnnnnnga
       61 aaaaagagca gtgggaatag gagctttgtt nnngggttct tgggancagc nnggaagcac
      121 tatgggcgca gngtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
      181 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
      241 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
      301 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
      361 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
      421 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
      481 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
      541 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
      601 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
      661 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccagaga
      721 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
      781 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
      841 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      901 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      961 aaacaccggg agccatcaca cagaccccgt tttagagcta gaaatagcaa gttaaaataa
     1021 gggtagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcttttnn gaat
//
