LOCUS       sequence-193225-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetic depletion of SLC19A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pLentiCRISPR sgSLC19A1
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     157..176
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        256..331
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    379..496
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        565..776
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      796..805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             805..828
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttttntnttn cttnnttttc nngnnatnnc gnnnnntnna tcggngntna aannngaatn
       61 atttgacngt nacacaaaga tatnnnncaa aatacgtgac gtagaaagta ataatttctt
      121 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      181 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcgagt
      241 tgaagaccca ccagagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      301 tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa ttctagatct tgagacaaat
      361 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      421 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      481 aaaaattcaa aattttcggg tttattacag ggacagcana gatccacttt ggcgccggct
      541 cgagtggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
      601 tggggggagg ggncggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      661 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      721 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccnca acacaggtgt
      781 cgtgacgcgg gatccgccac catggattac aaagacgatg acgataagat ggccccaaag
      841 aagangcngn annngnnnnn natccacgng agtcccagca gccgacaaga agtacagcat
      901 cggc
//
