LOCUS       sequence-193226-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetic depletion of HAL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pLentiCRISPR sgHAL
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..159
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        239..314
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    362..479
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        548..759
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      779..788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             788..811
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gnntatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
       61 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      121 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      181 cttggcttta tatatcttgt ggaaaggacg aaacaccggc aggcagctca ctgagggagt
      241 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      301 caccgagtcg gtgctttttt gaattctaga tcttgagaca aatggcagta ttcatccaca
      361 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
      421 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
      481 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagtgg ctccggtgcc
      541 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      601 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      661 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      721 aacgttcttt ttcgccacgg gtttgccgcc agaacacagg tgtcgtgacg cgggatccgc
      781 caccatggat tacaaagacg atgacgataa gatggcccca aagannannn nnaangtcgg
      841 tatccacgga gtcccagcag ccgacaagaa gtac
//
