LOCUS       sequence-193227-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetic depletion of AMDHD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pLentiCRISPR sgAMDHD1
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..159
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gcntatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
       61 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      121 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      181 cttggcttta tatatcttgt ggaaaggacg aaacacccat ggaaattcac caggccgggt
      241 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gnnanagtgc
      301 caccgagtcg gtgctttntt gantcnnntc ttgagaaaat gncattattc naacaggatt
      361 ntgannnaga gggggccgtn gngngtgggt ggacncganc atctctcggg gaaaattttg
      421 aactctgttg anngaaaatc tgacnnnagt tggaaacgcg agtcnaccaa gtcccctaan
      481 tggantgggt aatcgcnccg tagnaaagaa ctcgttgtac aacngtgtga gaagtcgnnn
      541 ccctggaaat ccacncagta acactccggt tacccctatg atcctgccga taagcgcnnn
      601 tataacccaa ttgtgttgng acnntnccat ttagagacat agtgtncnct tgtgncngnt
      661 ctcaagcacg gtgacanagt gcgnncagat gctntcatgt nangtaang
//
