LOCUS       sequence-193228-       12744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse cDNA of FTCD, refractory to the guides in 
            plasmids 1,2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 12744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pWP1_mFTCD
FEATURES             Location/Qualifiers
     source          1..12744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5177..5744
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5195..5746
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5339..5356)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5566..5585
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5749..5751,5752..5754,5755..6465)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5749..5751,5752..5754,5755..6465)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5794..5815)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6055..6074)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6402..6423
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6544..6560)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6545..6561)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6574..7162
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6627..6647)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7045..7056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7306..7339)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7359..7539
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7564..7582)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7565..7582)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7872..7891)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8105..8124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8224..8246
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8284..8302)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8370..8474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8475..8543,8544..9335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8693..8712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9506..10094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9995..10014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10248..10265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10335..10355)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10340..10669
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10520..10655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11803..11868
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11998..12018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12443..12577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12480..12499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12534..12553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaacctcgag gccaccatgt ctcagttggt ggaatgatgt ctcagttggt
     3541 ggaatgtgtc cccaacttct cagaggggaa caaccaggag gtgattgatg ccatctctcg
     3601 agccatctcc cagaccccag gctgtgtgct gttggacgtc gatgccggac cctccaccaa
     3661 ccgcactgtc tacactttcg tggggcagcc agagtgtgtc gtagaggggg ccctccatgc
     3721 tgcgcgtacg gcctcacagc tcatcgacat gagcaagcac aagggtgagc accctcgaat
     3781 gggcgctctg gatgtgtgtc ccttcatccc ggtgaggggc gtcagcatgg aagagtgtgt
     3841 gctctgtgcc aaggcatttg ggcagcggct ggcagaggaa cttaatgtac cagtgtatct
     3901 ctatggtgag gcagctcaga cgcccagtcg tcagaccctg ccagccatcc gggctggaga
     3961 gtatgaggcc ctgcctgaga agctcaaaca ggccgagtgg gtgccagact ttggccctag
     4021 ctcctttgtc cccagttggg gtgctactgt cacgggtgca cggaagttcc tcattgcatt
     4081 caatatcaac ctgctaagca ccaaggagca ggcccaccgt attgctctta acctgcggga
     4141 gcagggccga gggaaagacc agcctggacg tctgaaaaag gtccagggca tcggttggta
     4201 cctggaagag aagaacctgg ctcaggtgtc cacaaacctc ctggactttg aggtcacagc
     4261 tctgcacaca gtgtttgagg aggcccgcag agaggctcag gagctgaatc tcccagtggt
     4321 gggctcgcag ctggtgggcc tggtgcctct gaaggcctta ctggatgccg cagccttcta
     4381 ctgtgacaag gagaagctgt ttgttctgga ggaggagcat cggatacggc tggtggtgaa
     4441 ccggctgggc ctggattcgc tggcaccctt cgacccaaag gaacggatca tcgagtacct
     4501 agtccccgat agtggaccag aacagagcct gttggacacc tcactgcgcg gttttgttcg
     4561 ggaggtggga gcccgctctg ctgccccagg aggaggttct gtagcagcag cggtagcagc
     4621 tctgggtgca gccctggctt ctatggtggg ccagatgacc tatggccggc gccagtttga
     4681 tcacttggat tccacaatgc gacgcctgat cccacccttc cacgcagcct ctgcccagct
     4741 gacttcattg gtggatgctg atgcccgggc gtttgctgcc tgtttggagg caataaagct
     4801 gcccaagaac acgcctgaag agagagacag gcgtgcatgc gctctgcagg aggggctgag
     4861 acaggcagtc gctgtgccac tgaaactggc cgagactgtg tcccagctat ggccagcgct
     4921 gcaggagctg gcccactgtg ggaacctgtc ctgcttgtct gacctgcagg tggcagccaa
     4981 agccttggag acaggtgtgt ttggtgcgta cttcaacgtg ctcatcaacc tgaaggacat
     5041 gacggatgat gtatttaaag agaagacaca ccatcgaatc tccagccttc tgcaggaagc
     5101 caagactcag gctgcgctgg tgctgggtag cctagaagcc cgaaaggagt gagtttaaac
     5161 tacgggctgc aggaattccg cccccccccc cctaacgtta ctggccgaag ccgcttggaa
     5221 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5281 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5341 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5401 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5461 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5521 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5581 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5641 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc
     5701 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggtgagcaag
     5761 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5821 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     5881 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5941 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     6001 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     6061 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     6121 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     6181 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     6241 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     6301 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     6361 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     6421 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct
     6481 cgactagcta gtagctagct agctagtcga gctcaagctt cgaattcgat atcaagctta
     6541 tcgcgatacc gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt
     6601 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6661 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6721 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6781 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6841 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6901 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6961 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     7021 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7081 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7141 tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg gtacctttaa gaccaatgac
     7201 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7261 aattcactcc caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc
     7321 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7381 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7441 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7501 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     7561 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     7621 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     7681 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     7741 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     7801 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     7861 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     7921 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     7981 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     8041 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     8101 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     8161 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8221 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     8281 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8341 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8401 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8461 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8521 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8581 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8641 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8701 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8761 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8821 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8881 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8941 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9001 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9061 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9121 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9181 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9241 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9301 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     9661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     9901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10141 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10261 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10321 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    10381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    10441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    10501 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10621 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    10681 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    10741 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    10801 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    10861 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    10921 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    10981 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    11041 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    11101 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    11161 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    11221 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    11281 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    11341 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    11401 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    11461 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    11521 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    11581 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    11641 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    11701 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    11761 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    11821 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    11881 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    11941 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    12001 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    12061 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    12121 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    12181 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    12241 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    12301 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    12361 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    12421 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    12481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12541 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12601 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    12661 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    12721 aaatatgtac tacaaactta gtag
//
