LOCUS       sequence-193229-       13065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse cDNA of HAL refractory to the guide in plasmid 
            4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pWP1_mHAL
FEATURES             Location/Qualifiers
     source          1..13065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5498..6065
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5516..6067
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5660..5677)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5887..5906
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6070..6072,6073..6075,6076..6786)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6070..6072,6073..6075,6076..6786)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6115..6136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6376..6395)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6723..6744
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6865..6881)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6866..6882)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6895..7483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6948..6968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7366..7377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7627..7660)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7680..7860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7885..7903)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7886..7903)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8193..8212)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8426..8445)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8545..8567
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8605..8623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8691..8795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8796..8864,8865..9656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9014..9033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9827..10415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10316..10335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10569..10586
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10656..10676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10661..10990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10841..10976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12124..12189
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12319..12339
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12764..12898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12801..12820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12855..12874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaacctcgag gccaccatgc ctaggtacac agtgcacgtg cgtggggaat
     3541 ggctggcggt gccctgtcag gatgggaagc tcactgttgg ctggctgggc cgtgaggctg
     3601 tgcgacgcta catgaagaac aaaccggaca atggtggctt cacttcggtg gatgaagtgc
     3661 aattccttgt gcatcggtgc aagggcctgg gcctgctgga caatgaggac gagctggagg
     3721 tggccttaga ggacaatgag ttcgtagaag tggtgattga aggtgatgtg atgtctcctg
     3781 atttcattcc gtcgcagcca gaaggagttt tcctatacag caaataccgg gagcctgaaa
     3841 aatacattgc cctagatggg gacagtctga gcacagagga tctggtcaac ctgggcaagg
     3901 gacgttacaa gataaagctc acttcaattg ctgagaagaa agtgcagcag tcccgggagg
     3961 tcatcgacag catcatcaaa gaaagaacag ttgtttatgg catcaccaca ggttttggaa
     4021 aatttgctag aactgtaatc cctgccaata agctacagga gcttcaagtc aacttagtcc
     4081 gttcccattc ttcaggtgtt gggaaaccac tcagtcctga gaggtgtcgg atgctcttgg
     4141 ctttgaggat caacgtctta gccaaaggtt acagtgggat ttccttggag acgctgaaac
     4201 aagttattga agcatttaat gcctcctgcc tgtcctatgt tccagagaaa ggaactgtgg
     4261 gtgccagcgg agaccttgcc ccactctctc atcttgctct ggggctcatc ggagaaggaa
     4321 agatgtggtc cccgaagagt ggctgggcag atgctaaata tgttctagaa gcccatggac
     4381 tgaaaccaat tgtcttgaaa ccaaaagaag gcctggcact catcaatggg acacagatga
     4441 tcacttccct gggctgtgaa gccctggagc gagccagcgc cattgcccgg caagctgata
     4501 ttgtggctgc cttgaccctg gaggtgctga agggcaccac caaagccttc gataccgaca
     4561 tccatgctgt ccgacctcac cgtgggcaaa ttgaagttgc tttccgattc cggtccctcc
     4621 tggactcgga tcaccaccca tcagaaatcg cagaaagcca caggttctgc gatcgtgttc
     4681 aggacgcgta caccttacgc tgctgtccac aggtccacgg tgtggtgaac gacacaatag
     4741 cctttgtgaa ggacatcatc actacagaac tgaacagcgc cacagacaat cctatggtct
     4801 ttgccagtcg aggggagaca atttcaggag gaaacttcca tggtgaatac ccagccaaag
     4861 ccctggacta tttggccatt ggtgtccatg aacttgcggc aattagtgaa agaagaatcg
     4921 aaaggctgtg taatccttcc ctcagcgagc tgcctgcctt cctggtggct gaaggaggtc
     4981 tcaattctgg gttcatgata gcccactgca ccgcagcagc cctggtgtct gagagcaagg
     5041 ctctgtgcca cccttcatct gtggactcac tctcgaccag tgctgctaca gaagaccacg
     5101 tctccatggg aggatgggca gccaggaaag ccctcagggt cgtcgagcac gtggaacaag
     5161 tgctagccat tgagcttctc gcggcctgcc agggtataga gtttctgcgc cccctgaaaa
     5221 caaccactcc gctggagaag gtgtacgacc tggtgcgctc cgtagtaagg ccctggataa
     5281 aagatcgctt catggccccg gacattgagg cagcacacag gttgcttctt gaccaaaagg
     5341 tttgggaggt tgccgcaccg tacatcgaga aatacagaat ggagcacatt ccggaatcca
     5401 gaccgctttc tccaacagcg ttttcactag aatctctccg aaagaactca gccacaatcc
     5461 cagagtctga cgagtttaaa ctacgggctg caggaattcc gccccccccc ccctaacgtt
     5521 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     5581 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     5641 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     5701 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     5761 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     5821 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     5881 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     5941 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     6001 ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6061 gataatacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     6121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     6181 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     6241 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     6301 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     6361 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     6541 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     6601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6661 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     6721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6781 tacaagtccg gactcagatc tcgactagct agtagctagc tagctagtcg agctcaagct
     6841 tcgaattcga tatcaagctt atcgcgatac cgtcgacctc gagggaattc cgataatcaa
     6901 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6961 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7021 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7081 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7141 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7201 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7261 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7321 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7381 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7441 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcgagctc
     7501 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7561 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc
     7621 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
     7681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7861 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     7921 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     7981 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     8041 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     8101 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     8161 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     8221 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     8281 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     8341 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     8401 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     8461 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8521 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     8581 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     8641 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8701 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8761 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8821 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8881 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8941 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9001 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9061 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9121 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9181 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9241 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9301 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9361 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9421 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9481 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9541 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9601 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9661 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9721 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9781 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9841 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9901 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9961 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10021 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10081 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10141 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10201 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10261 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10321 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10381 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10441 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10501 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10561 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10621 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
    10681 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    10741 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10801 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    10861 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    10921 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    10981 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
    11041 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
    11101 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
    11161 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
    11221 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
    11281 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
    11341 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
    11401 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
    11461 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
    11521 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
    11581 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
    11641 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
    11701 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
    11761 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
    11821 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
    11881 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
    11941 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
    12001 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
    12061 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
    12121 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    12181 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
    12241 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
    12301 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
    12361 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
    12421 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
    12481 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
    12541 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    12601 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    12661 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    12721 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    12781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    12841 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    12901 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    12961 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    13021 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtag
//
