LOCUS       sequence-193235-       18841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDSW204 carrying genes entABCDE, vibHF and a cos site, for 
            serratiochelin and analogs production in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18841)
  AUTHORS   Cleto S, Lu TK
  TITLE     An Engineered Synthetic Pathway for Discovering Nonnatural 
            Nonribosomal Peptides in Escherichia coli.
  JOURNAL   MBio. 2017 Oct 10;8(5). pii: e01474-17. doi: 10.1128/mBio.01474-17.
  PUBMED    29018120
REFERENCE   2  (bases 1 to 18841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2017-10-10"; volume: "8(5). pii: e01474-17. doi"; pages: " 
            10.1128/mBio.01474-17"
COMMENT     Sequence Label: pEV_S
FEATURES             Location/Qualifiers
     source          1..18841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..33
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     267..284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    299..439
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(365..384)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     525..547
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        625..702
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             703..1785
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(722..741)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(2017..2022,2023..2039,2040..2046)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2054..2070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2059..2081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             2721..2732
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             7156..7167
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             8495..8506
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    16760..16816
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(16849..16866)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      17019..17105
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      17197..17224
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        17243..17334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(17335..17403,17404..18195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(18366..18841,1..113)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
       61 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      181 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      241 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      301 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
      361 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
      421 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      481 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      541 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa
      601 ggcgaagcgg catgcattta cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg
      661 gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta
      721 tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag
      781 gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat
      841 tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt
      901 gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
      961 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
     1021 tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
     1081 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta
     1141 tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
     1201 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
     1261 ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
     1321 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
     1381 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
     1441 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
     1501 gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta
     1561 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
     1621 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
     1681 aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1741 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     1801 tgtaagttag cgcgaattga tctggtttga cagcttatca tcgactgcac ggtgcaccaa
     1861 tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
     1921 tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
     1981 tcataacggt tctggcaaat attctgaaat gagctgttta caattaatca tccggctcgt
     2041 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagaca ctagtatgaa
     2101 aactacgcat acctccctcc cctttgccgg acatacgctg cattttgttg agttcgatcc
     2161 ggcgaatttt tgtgagcagg atttactctg gctgccgcac tacgcacaac tgcaacacgc
     2221 tggacgtaaa cgtaaaacag agcatttagc cggacggatc gctgctgttt atgctttgcg
     2281 ggaatatggc tataaatgtg tgcccgcaat cggcgagcta cgccaacctg tctggcctgc
     2341 ggaggtatac ggcagtatta gccactgtgg gactacggca ttagccgtgg tatctcgtca
     2401 accgattggc attgatatag aagaaatttt ttctgtacaa accgcaagag aattgacaga
     2461 caacattatt acaccagcgg aacacgagcg actcgcagac tgcggtttag ccttttctct
     2521 ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcaagtg agatccaaac
     2581 tgatgcaggt tttctggact atcagataat tagctggaat aaacagcagg tcatcattca
     2641 tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa gaaaagatag tcataacgct
     2701 gtgccaacac gattaacatt aaagaggaga aaggtaccat ggatacgtca ctggctgagg
     2761 aagtacagca gaccatggca acacttgcgc ccaatcgctt tttctttatg tcgccgtacc
     2821 gcagttttac gacgtcagga tgtttcgccc gcttcgatga accggctgtg aacggggatt
     2881 cgcccgacag tcccttccag caaaaactcg ccgcgctgtt tgccgatgcc aaagcgcagg
     2941 gcatcaaaaa tccggtgatg gtcggggcga ttcccttcga tccacgtcag ccttcgtcgc
     3001 tgtatattcc tgaatcctgg cagtcgttct cccgtcagga aaaacaagct tccgcacgcc
     3061 gtttcacccg cagccagtcg ctgaatgtgg tggaacgcca ggcaattccg gagcaaacca
     3121 cgtttgaaca gatggttgcc cgcgccgccg cacttaccgc cacgccgcag gtcgacaaag
     3181 tggtgttgtc acggttgatt gatatcacca ctgacgccgc cattgatagt ggcgtattgc
     3241 tggaacggtt gattgcgcaa aacccggtta gttacaactt ccatgttccg ctggctgatg
     3301 gtggcgtcct gctgggggcc agcccggaac tgctgctacg taaagacggc gagcgtttta
     3361 gctccattcc gttagccggt tccgcgcgtc gtcagccgga tgaagtgctc gatcgcgaag
     3421 caggtaatcg tctgctggcg tcagaaaaag atcgccatga acatgaactg gtgactcagg
     3481 cgatgaaaga ggtactgcgc gaacgcagta gtgagttaca cgttccttct tctccacagc
     3541 tgatcaccac gccgacgctg tggcatctcg caactccctt tgaaggtaaa gcgaattcgc
     3601 aagaaaacgc actgactctg gcctgtctgc tgcatccgac ccccgcgctg agcggtttcc
     3661 cgcatcaggc cgcgacccag gttattgctg aactggaacc gttcgaccgc gaactgtttg
     3721 gcggcattgt gggttggtgt gacagcgaag gtaacggcga atgggtggtg accatccgct
     3781 gcgcgaagct gcgggaaaat caggtgcgtc tgtttgccgg agcggggatt gtgcctgcgt
     3841 cgtcaccgtt gggtgagtgg cgcgaaacag gcgtcaaact ttctaccatg ttgaacgttt
     3901 ttggattgca ttaaggagcg aggatgagca ttccattcac ccgctggccg gaagagtttg
     3961 cccgtcgcta tcgggaaaaa ggctactggc aggatttgcc gctgaccgac attctgacgc
     4021 gacatgctgc gagtgacagc atcgcggtta tcgacggcga gcgacagttg agttatcggg
     4081 agctgaatca ggaggcggat aacctcgcgt gtagtttacg ccgtcagggc attaaacctg
     4141 gtgaaaccgc gctggtacaa ctgggtaacg tcgctgaatt gtatattacc tttttcgcgc
     4201 tgctgaaact gggcgttgcg ccggtgctgg cgttgttcag ccatcagcgt agtgaactga
     4261 acgcctatgc cagccagatt gaacccgcat tgctgattgc cgatcgccaa catgcgctgt
     4321 ttagcgggga tgatttcctc aatactttcg tcacagaaca ttcctccatt cgcgtggtgc
     4381 aactgctcaa cgacagcggt gagcataact tgcaggatgc gattaaccat ccggctgagg
     4441 attttactgc cacgccatca cctgctgatg aagtggccta tttccagctt tccggtggca
     4501 ccaccggcac accgaaactg atcccgcgca ctcataacga ctactactac agcgtgcgtc
     4561 gtagcgtcga gatttgtcag ttcacacaac agacacgcta cctgtgcgcg atcccggcgg
     4621 ctcataacta cgccatgagt tcgccaggat cgctgggcgt ctttcttgcc ggaggaacgg
     4681 ttgttctggc ggccgatccc agcgccacgc tctgtttccc attgattgaa aaacatcagg
     4741 ttaacgttac cgcgctggtg ccacccgcag tcagcctgtg gttgcaggcg ctgatcgaag
     4801 gcgaaagccg ggcgcagctt gcctcgctga aactgttaca ggtcggcggc gcacgtcttt
     4861 ctgccaccct tgcggcgcgt attcccgctg agattggctg tcagttgcag caggtgtttg
     4921 gcatggcgga agggctggtg aactacaccc gacttgatga tagcgcggag aaaattatcc
     4981 atacccaggg ttacccaatg tgtccggatg acgaagtatg ggttgccgat gccgaaggaa
     5041 atccactgcc gcaaggggaa gtcggacgcc tgatgacgcg cgggccgtac accttccgcg
     5101 gctattacaa aagtccacag cacaatgcca gcgcctttga tgccaacggt ttttactgtt
     5161 ccggcgatct gatctctatt gatccagagg gttacatcac cgtgcagggg cgcgagaaag
     5221 atcagattaa ccgtggcggc gagaagatcg ctgccgaaga gatcgaaaac ctgctgctgc
     5281 gccacccggc ggtgatctac gccgcactgg tgagcatgga agatgagctg atgggcgaaa
     5341 aaagctgcgc ttatctggtg gtaaaagagc cgctgcgcgc ggtgcaggtg cgtcgtttcc
     5401 tgcgtgaaca gggtattgcc gaatttaaat taccggatcg cgtggagtgt gtggattcac
     5461 ttccgctgac ggcggtcggg aaagtcgata aaaaacaatt acgtcagtgg ctggcgtcac
     5521 gcgcatcagc ctgaaggaga gaacacgatg gctattccaa aattacaggc ttacgcactg
     5581 ccggagtctc acgatattcc gcagaataaa gttgactggg cctttgaacc gcaacgtgcc
     5641 gcgttgttaa tccatgatat gcaggactat tttgtcagct tctggggcga gaactgcccg
     5701 atgatggagc aggtgatcgc gaatattgct gcgctgcgcg actactgcaa acagcacaat
     5761 atcccggttt attacaccgc ccagccgaaa gagcagagcg atgaagatcg ggcgctgttg
     5821 aatgatatgt gggggccggg cttgacccgc tcgccggaac agcaaaaggt ggtggatcgc
     5881 ctgacgccag atgccgacga cacggtgctg gtgaagtggc gctacagcgc gtttcatcgt
     5941 tctccgctgg agcaaatgct gaaagagagt ggacgtaacc agctgattat taccggggta
     6001 tatgcccaca ttggctgtat gaccaccgca accgacgcat ttatgcgcga tattaaaccg
     6061 tttatggtgg cggatgcgct ggccgatttc agccgtgacg agcatttgat gtcgctgaaa
     6121 tatgtggccg gacgttctgg ccgggtggtg atgactgaag aattactgcc agcacctatc
     6181 cccgccagca aagcggcgct gcgtgaggtg atcctgccgt tgctggacga gtccgatgaa
     6241 ccgttcgatg acgacaacct gatcgactac ggtctggatt cggtgcgcat gatggcgctg
     6301 gcggcgcgct ggcgcaaagt gcatggtgat atcgactttg tcatgctggc gaaaaacccg
     6361 accatcgacg cctggtggaa gctactctcc cgcgaggtga aataatggat ttcagcggta
     6421 aaaatgtctg ggtaaccggc gcaggtaaag gtatcggcta cgccacggcg ctggcgtttg
     6481 ttgaggcggg agcgaaagtt acaggttttg atcaagcgtt cactcaggag caatatccct
     6541 ttgcgaccga agtgatggat gttgccgacg ctgcgcaggt cgcgcaagtg tgtcagcgac
     6601 tgttagctga aacggagcga ctggacgcgc tggtcaatgc ggcgggaatt ttacgcatgg
     6661 gcgcgaccga tcagctcagt aaagaggact ggcagcagac ttttgcggtt aacgtcggcg
     6721 gtgcgtttaa cctgttccag caaaccatga accagtttcg ccgtcagcgg ggcggggcga
     6781 ttgtcactgt ggcgtccgac gccgcgcaca cgccgcgtat tggcatgagt gcttatggcg
     6841 catcgaaagc ggcgctgaaa agcctggcgt tgagcgtcgg gctggaactg gcgggtagcg
     6901 gcgtgcgctg taatgtggtt tcgcctggct ccaccgacac cgatatgcga cgcacgctgt
     6961 gggtgagcga tgacgccgaa gaacagcgta ttcgcggctt tggcgagcag tttaaactcg
     7021 gcattccgct ggggaaaatc gcccgtccac aagagatcgc caacacgatt ttgttcctcg
     7081 cctctgacct cgccagccat attaccctac aggatattgt ggtcgatggc ggctcaacgc
     7141 tgggggcata acattaaaga ggagaaaggt accgtgtcta tgttattggc tcaaaagcct
     7201 ttttggcagc gccatctggc ttatcctcac attaatctcg acaccgttgc ccattcgcta
     7261 cgcctgacag ggcctttaga taccacactt ttgctgcgcg cattgcattt aaccgtcagt
     7321 gagatagatc tgttccgtgc tcgcttttct gcgcaaggtg agctgtattg gcacccattt
     7381 tctccgccta tcgactatca agacctcagc atccaccttg aagcagaacc tttagcttgg
     7441 cggcaaatag agcaggattt acagcgctca agcacactga ttgacgcgcc aataacgagc
     7501 catcaagtgt atcgtttgtc acacagtgag caccttatct atacccgtgc gcaccacatt
     7561 gtgctggacg gttatggcat gatgctgttt gagcagcgcc tcagccaaca ttaccaatcc
     7621 ttgctcagtg ggcagacgcc aactgccgcg tttaaacctt atcaatccta tctggaagaa
     7681 gaggcggctt atcttaccag ccatcgctac tggcaagata agcagttttg gcaaggctat
     7741 ttacgcgaag ctcccgactt aacgctcacc tctgcaacct atgatcctca acttagtcat
     7801 gccgtgagcc tctcttacac actcaatagc caactcaacc atttgttact caagctcgct
     7861 aacgcgaatc agattggctg gcctgatgcc ttagtcgcgc tgtgcgcgct ctatttggaa
     7921 tcggctgaac ctgatgcgcc ttggctgtgg ctgcccttta tgaaccgatg gggcagcgtg
     7981 gccgctaatg tgcccggttt aatggttaac tcgctgccct tgctgcgtct ttctgcgcag
     8041 caaacctcgc tcggcaacta ccttaaacag tcagggcaag cgatacgctc actctatttg
     8101 catggccgct atcgaattga acaaatcgaa caagatcaag ggctaaatgc tgagcaaagt
     8161 tattttatga gcccatttat caatattttg ccttttgaat ccccacactt tgctgattgc
     8221 cagactgagc tcaaggtgct ggcttcaggc tctgcagaag ggattaattt tacctttcgt
     8281 ggctcacccc agcatgaact ctgtctagac atcaccgcag atttagccag ctacccgcaa
     8341 tcccactggc aaagtcattg cgagcggttt ccacgttttt tcgagcagtt actcgcccgt
     8401 ttccaacagg tggagcaaga tgttgcacgc ctactcgcag agcctgcggc attggcagcc
     8461 accacctcca cacgggctat cgccagttga cattaaagag gagaaaggta ccatgaaaga
     8521 aatgacagca atgcaagcgg cttattggct agggcgtcaa cacgactgct tgctcgatgg
     8581 tgtggccgcg catctctacg ccgaatttga tggtcaagca ttaaatcgac aggcgctaac
     8641 ggaagcggta cgtgcgctgt acgcgaaaca tcccatgtta cgtttagcga tcaccaagga
     8701 tggacagcag aagatcttgc cgctctcaac tttccatcag cttaaagtgg atgacctatc
     8761 acaatggaag ccagatgagg tggagtcttt tgtgcatact aagcgccagc gaatgaccca
     8821 ccagatgctc gatttaactc agggaaaccc gattgagatc agcttgactc tgttgcctga
     8881 gggcaaacac cgattacaca ttgatgcgga tatgattgcg tgtgatgcac aaagttttcg
     8941 gctgttggtg gacgatctca cctcactcta tttagaggcc atagagcatc gattagagat
     9001 cattgagtct gatgtggtga cttttttcca atatcttgat gctcagcaag ccgatcgtgc
     9061 tttggcaaaa cgcaaagagg tggataagaa atggtggcaa gagcgtcttg cgacgattcc
     9121 cgccgagcca agtttgcctt atcaacccgt accaaccgac gccgttagcg caaacagcca
     9181 aaggtttgca cactggttca ctccagtgga gcgcaaaggg ttggcggagg ttgcgcggca
     9241 acatcatctc acactgaccc aactgacatt ggcactcttt tcacaagtga ttgccaatgc
     9301 ttgccaagag agacaatttc gactcaatgt acctacattc catcgcggca atcgctcttt
     9361 ggatattgaa cacacgatcg gcgatttttc caatttgctg atttttagcg ccgatgtggg
     9421 aacgactcag accctgttaa gcctgtgtca gcaaacggct aaccaactgc atcaattact
     9481 tcgccatgaa agctattcag gggttagcgt gatgcgcgat ttatcgcgga aacagggtgg
     9541 ggtgcagcgt tcaccgatag tctttacctc aggcatggaa atgcgagacg aggagatctt
     9601 ttccgatcat gtcactcagc atttagggcg tatgaattgg gtgatttcgc agggagcgca
     9661 ggtgactctc gatgcgcaaa ttgctccggc ttatgaaggg attttgctca attgggatgt
     9721 gcgaatggaa aacttcgctg ataaggacat cacagcgctt ttcgcgcatt acgttgattt
     9781 gatccgctgt gtggcgctgc atcctgagat gatgcagcaa agcgtacaac agattgacgc
     9841 gcaactcggt tatgctcgcc gcgagtcgat tcaggagatg ccactgaccc ctttacaaca
     9901 agcttacttg ctaggtcgct caacccaaat tgccttaggc ggcgtcgcga tgcatgagtt
     9961 tcgtgagtat cgaggtcaca tcgacactca gtcactgcat agccgtctgc tctatctcgt
    10021 tgagcatatt cctgccttga gaactcgcat tgatcaagag aaatggattc agtgggtctc
    10081 tccttgcata gcgttaaatt ggcaagccat cgatttacag catctttccc gcgaacaagc
    10141 cttattagcg gttgagccag tcaggcagca atatcagcag cggatgcatg atttaacgcg
    10201 ctcgccctgg caaatctgtg tggtgcaact gccaatagaa gagcaagagg agttcagctc
    10261 catcgtattg accagctttg atgctttgat tgtcgatggt cgcacccatg cactcattct
    10321 tgctgcacta ctaggaagcg aagaacccga tataactcag gtggtgcaaa atgctcgtga
    10381 tactcagtcc atctcgccgc aattcgcctc caaaaaagcg caagatgaag cgtattggaa
    10441 aagcaaactt catcctgatt gcccgccgcc agcactgcct tggaaacaag cattagagac
    10501 gatcactacg tctcgttatg ctcgtgaaag tttgcaaata cccaaagaga gtgtcggtaa
    10561 gctcaatcga tgcggcattg aaaatggctt atttctcaat tccttgctga cagcaaccat
    10621 cttggacgtg ctctcctatt ggacaaccga aatcgggatg cgagtgggtt ttccggtatt
    10681 aattccaagc agtaatgcga ttgatggcaa tgagtcatcc tttgtgatct tagagcatga
    10741 aaaatccacc ctgagtttgc tgagccaagc gagcaagcta cagcgtgaga tgctagaggc
    10801 tcttgagcat cttgcttttt ctggcgtcga tcttaaccgt ctgttgatga atcaagcgcc
    10861 gcaagctctg gtgctgccgg ttgtgttaac caatggtttg tcttggaaaa ccctgaatcc
    10921 agaggacgct gtcactctgt tcgatggggt aacgcaaact ccgcaggtgg ctttagatat
    10981 tcgcttaact tacgacgagc agaaaaacct catcatcagc tttgattatg ctttagcggt
    11041 tttagagact gagcttatcc gcgagatgtt aagtgcctta catcatcgct tgagtcagat
    11101 cacttcgtca gcatcattgg ccgcgccgct tgagccatgt atcgaccttt cacactaccg
    11161 ttttaatagc gatgagtcgg ccagtcacga ttgcgatttt cttgccaagt tagcgcagca
    11221 attgtttgtc cgcacagagg ataaaaccgc tgtgatctgc ggtgaacaaa ccctttctta
    11281 tgctcaattg ggtgagcagg ttcagcgcgt gatgtggcag ctcaaagcgc gagggttgac
    11341 cacaggaaat gtgctcgcca tttgcttgcc acgcagtgtt gaacatatcg tcatcagttt
    11401 ggcttctgca ctttcgggga ttatctgggt acctattgat gctgcgtctc ccaaagagcg
    11461 tctgaattac cttttagaaa attgtcacgc cgatctggtg gtgatggata agccctgtga
    11521 gtttggcaat gtgatcgctt ttgatgcctt gattgagcct gtcttattcg ccgatggagt
    11581 acctgacgtt acaccactcg atcaattaag ccgattaagc caaagccaac aaacggctta
    11641 ttatctttat acctctggca ctacgggcaa acctaagtgc gtggtggtca ataatcaggc
    11701 gacctcgaat gtgattggtc aaactggcca agcgtggcat ctcaccagtg aggatgtggt
    11761 gatgtcagtg acgccattgc atcatgatat gtcggtgttt gatctctttg ccaccttgag
    11821 cttcggagcg acgttagttc ttcccgctgg gcatgaagaa aaagatgcgt tgcaatggaa
    11881 tcgcttaatt gagcggcatc aagtgacgat ttgggtctcc gtccccgcta tcttggagat
    11941 gttgctctct tgcactcaag cagggcaatt acactcactt cgtctgattg cccaaggggg
    12001 ggattacatc aaacctgcca ccatcgccca attacgcgct ggttcaaatc cgccgcggtt
    12061 gatctctttg ggcggtccta ccgagacgac gatttggagc atctggcatg agctgacggc
    12121 agatgatgtc agcgtgatcc catatggtcg gccgttggcg ggaaaccgtt atttcattat
    12181 ggatgagata caacgccatg taccgcaagg tgtggtcggc cgtatcttca cctctggggt
    12241 caatttagcg caaggttatc ttgaagacgg cgaactgaaa caaaccgatt ttgtcaccgt
    12301 attggatgag catggccacc ctgtgcgcgc gtttcgaacg ggcgatcaag gctactaccg
    12361 cgcggacggc aatatcattt ttgctagccg catcaatggt tatgtgaaag ttcgtggggt
    12421 acgagtgtca ctgccggata tcgaaaagca gctgcaaact catccggcgc tcgcgagcgt
    12481 ggtggtggtc gattatgcgg atactaatgg agataccgct ttagccgcat tatttagcgt
    12541 taaacctcag caatcagcat cgagtcaggc gttacgagag tttgccaaac aatcgctacc
    12601 gtcttcgcat attccgagtc ggtttattgc tttagaggct ttaccgcttt ctgcgaatgg
    12661 taaagtggat cgcaaacagt gccaagcgca tgttcagcga caatcgatct ctgttgaacc
    12721 agtaggtcaa ccgaatcaaa gtcaacctct ctcaccacct accttgtcat cggcttcctt
    12781 gagtgagttt gaacagcgag tctggcgaca gcatcaacga catggtgacg gacgcaatca
    12841 atatgcgacc gcttatcgat tgagcggaaa agtgaatatc gcgcgcttaa tcacagcgct
    12901 cagtcagttg cccaatcatt ttccagtgtt aaaccgacgc tatgtgcttg atgaggcatc
    12961 cggcttaacc ttgtactcgg cgaagccaac accgcttgag atccattttg agcatgttga
    13021 gtcaatggat gaggcgtttg aacatcttgt gcgatggcaa aatcagccga tggatttagc
    13081 caagcaagcc acactaagtt tttgcttgtt gagtttgggg agcgaagagc gagtgcttgg
    13141 ggtgataagc catcaaatta tcagcgagca atgggattgg cggcgcgtat ttgagtgcgt
    13201 aaccaatggg tataaccaaa tcgcctgtga catcgatcct gtgatggagg gggaggatct
    13261 ttccctcggc tttacccctg tgatgccgca aacgccatta tcccaagcgt tgttgccgtg
    13321 gttacagcct gcgagccacg ctacatggat tgagcagagc ttgccgagcg caaatagcgc
    13381 catcaatacg ctttgcagtc gtaaatatcg gattgcccta cacagcagcg tactggcgga
    13441 gcatggttta gcgcagccgg ataagcagga agtgcttgca ctgatcgccg cgttatttgc
    13501 tcgctacctt gccgcaagtg cgaaacttgg ccaatttgag ctttacgttc cgcatgatgt
    13561 ggagcgcaaa acgcgtgagt tgaatggttc tatgattgaa agccagttgg tgcatatcgc
    13621 ccttaaaggc tttgagcgtc cgctttcgca actgagccaa gagatcttag acgctatgcg
    13681 ccagccgtta acggggggag tcgctagcga aactcacgcc tctgccgcag ccttggtgac
    13741 ttggttagtg gatccttcag tgcatctaca attggagggc ttgcgctgcg aaaaattact
    13801 ttttgcagcc atgcacccca aatttgaagt cgccttgggg gttgggctta attgccaagg
    13861 tgccctagta cttgagctgg cgttagatgc cacggtttcc ccccatgtcg gagcctactt
    13921 gcttgagcaa tttgtcgctg cgataggtgg gcgaacaatg ccttcttcga caacctcatc
    13981 ctctcatgtc tcagccattg agttgacgtc aaacaatccg ccgacctcaa acaatgcgca
    14041 tgcggcgttg gcagagtctg agcttgaatt gagcgctgta aattcctctg cggttgaaga
    14101 gtgcattttg gctgagtttc gctcagcact tggtgttgcc gagatgacgg cagaggatga
    14161 tttctttgat tttggcggac attccctgat cgcaacgcga gtcattggtc gcttgttgag
    14221 cgagcaaggt attgaactgc acatcaatga tatgtttagt ttccctaatg ccaagcaact
    14281 ggcgcagcaa gccgtacttc accgtaaacc caccagcaca tcattcgcag tgagtgaggt
    14341 ggtcgagtcg tccaaagcgc ctttgtcgtt agcacaagcc tctttatgga aggcgatgag
    14401 caaatacgcc aagtttggtt tgacccacat cttcaatctt ccgtttgcgc ttaaattttt
    14461 agatgaggtc aatgagcaag catttggtga ggcttttcac tggcttttac tgcgtcatgc
    14521 tgggttacga acccactttg gcctagaaga tgggcagcct taccagcacg tgatcgcagc
    14581 cagcaacata gaacattacc agtggttctg gacgtctaaa gataacgccg cccaatccgt
    14641 ggccagacta ctggcgcaag aggccgagca cacctttgac ctgagccaag aattgccact
    14701 tcgactcaat ttcgtacgtg atgaacagac ggggacgcaa tatctttcac ttctgttcca
    14761 ccatattgtg ttggatgagt ggtcgatcaa tattttgatg gatgaattag cacaggtgta
    14821 tcaacacagt gtgcaaggta ctcgtccaca gtggcaaacg gaacccttac ctttccatga
    14881 gtttgctcgc aagcagcgtt cttcggcatt caatcaaacg catcttaact attggttaac
    14941 caagtttgcc ggtgtgcctt gggcacagcc tctgtttgct gcggatcatc ctttaagtaa
    15001 cagcactggc gtcgatcttg gagaaggcgg ctgggtcgaa ataaaattgc cgaaatcaac
    15061 catggtatcg ctgtatcaat tggctaaagc acgccatgct tcgttgttta acgtgatgta
    15121 tgcggcgatt tctgcgtcgg tacattgtct cggcgcacca gaaaagctgc tggttggcac
    15181 acctgcttcc ggtcggctgg atgccgagtt tttcgacaca gtcggttatt tcactaccat
    15241 gggcgttcag ttggtggatt tcacgaaagt acaaacggtg tggcaactca tcgagcaagt
    15301 gaaaaacagc attaaccagt cgatgccata cacagatatt ccgattgacc tgattgaaga
    15361 agggctcaaa ggtgttgagc atgagacgga aggtcacatg tttgaggtct ttatacaact
    15421 gcatgcaaaa aataagctgc atggagagct actgttgaca gaagggcatg cgatccgctt
    15481 ccaacaagtc gatcccgata aaagtgaatc cggcttaggt ttacagtttg aaattttaga
    15541 agagaggatc gagcaaaagc agacgttgag agtcatgatg agttatatgt cgaaacacta
    15601 tagcccagct caagtcgctc tgttgaccaa ggtaaccagt ggcatgtttg agcggttttc
    15661 cgattgtatt gcacaagata ttgcactgcc gacgctgaaa aagcaggtga ggcagcttga
    15721 agatgaagct tgtcgctctc cgtccatggg gtagtatcgt ccattccgac agcatcgcca
    15781 gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc
    15841 tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag
    15901 ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatctgc ctcgctggcc
    15961 tgccgcagtt cttcaacctc ccggcgcagc ttttcgttct caatttcagc atccctttcg
    16021 gcataccatt ttatgacggc ggcagagtca taaagcacct cattaccctt gccaccgcct
    16081 cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact cgcaccgaaa
    16141 atgtcagcca gctgcttttt gttgacttcc attgttcatt ccacggacaa aaacagagaa
    16201 aggaaacgac agaggccaaa aagctcgctt tcagcacctg tcgtttcctt tcttttcaga
    16261 gggtatttta aataaaaaca ttaagttatg acgaagaaga acggaaacgc cttaaaccgg
    16321 aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtaacaag gcggatcgcc
    16381 ggaaaggacc cgcaaatgat aataattatc aattgcatac tatcgacggc actgctgcca
    16441 gataacacca ccggggaaac attccatcat gatggccgtg cggacatagg aagccagttc
    16501 atccatcgct ttcttgtctg ctgccatttg ctttgtgaca tccagcgccg cacattcagc
    16561 agcgtttttc agcgcgtttt cgatcaacgt ttcaatgttg gtatcaacac caggtttaac
    16621 tttgaactta tcggcactga cggttacctt gttctgcgct ggctcatcac gctggatacc
    16681 aaggctgatg ttgtagatat tggtcaccgg ctgaggtgtt tcgattgccg ctgcgtggat
    16741 agcaccatta ctagtcatgg aattctagct cggtacccgg ggatcctcta gagtcgacct
    16801 gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
    16861 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
    16921 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
    16981 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
    17041 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
    17101 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
    17161 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
    17221 tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg
    17281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    17341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    17401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    17461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    17521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
    17581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    17641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    17701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    17761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    17821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    17881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    17941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    18001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    18061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    18121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    18181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    18241 cctcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    18301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    18361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    18421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    18481 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
    18541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    18601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    18661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    18721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    18781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    18841 a
//
