LOCUS       sequence-193237-        3530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  drives expression of rpaA from an rpaA-independent promoter 
            (kanamycin resistance casette).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: P0050::rpaA (KmR)
FEATURES             Location/Qualifiers
     source          1..3530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..106
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1757..1776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1841..1860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cctcctagaa gctcatcagc gagatctctg gcaacaggtc agtccggcac tccttgatca
       61 actgcgatcg ctttcccttg aagcagaagc tgatattgaa ggtcgactag gaagtattcc
      121 agataacaag ttgcgctagt tggactttat tacaagcagt tgcaatcttc ggacgattct
      181 gctgtcgctg gttaccgtag aactattcct aatgcttgct caatccctat gagttcactt
      241 gttgttgtcg gatttcccaa ttcgcaagaa gccgaagaag tccgaatcaa acttgctcag
      301 ctacagacag aacatttaat tgccctagaa gatgctgttg ttgtcagcaa ggattccgag
      361 ggccatgtca aactcaatca agcgattaat ttaacggcaa ctggtgccgt cagcggcggt
      421 ttttggggca cccttgtcgg cttgatcttc ctcaatccct tggcgggtgc ggcgatcggt
      481 gcagcggctg gtgcactcag cggttccttg agcgacattg gcatcaacga tgactttctg
      541 cgcgaggtcg gcgaaaccct gcaggtcgga tcagccgccc tctgcctcct catccgcgaa
      601 gcaacccccg atcgcatcct gaaagagttg cgagtctacg ccaaagacgc ccggattcta
      661 caaacgaact tgagctttgc cgatgaagaa aacctcaagg cagccctaga aaaagccaaa
      721 gccgaagccc aagccctgcg tctcaattaa tctccaacgc ttcgatcgct cctgattact
      781 tgagttgatc aaggctcaga tcaagagcga tcgctacttc aatacagctt acttttggcc
      841 gctatctcaa cagcagttca atcctaagaa actgcagaag cagggattga gtaatttgat
      901 ggttgactgc agtccttatc agttgcttct caacaattat ccaaccgcaa ttacggacag
      961 agcactggac tagcaccact gctaaaaccg caaagctcgg ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc aggctgcctt tggttgcaat
     2041 aacggtaaat ttaccgtctg tcccgattgt gccagtcacg ttaatattcg ctgctttacc
     2101 acttccagca gccgttactt ggctagcatt gccgcaagtt atgttaaagt tacctgcact
     2161 gctaccaata ccaagctgtg tacaagacgc attggtgacg tagttaggat ctagtgtcgc
     2221 cgcactaagg gtacgaagag cagcaccgac tgctgatttg ccttcagagg cataggcttt
     2281 atctgtttga tccaacaggt tcggtaatgc aactgctgcc aagatgccaa cgataatgat
     2341 aacgaccagc aactcgacaa gggtaaaacc tttttcttct tcaccttgtt cgtctttacg
     2401 ctgggcaaga tattgtagat aacgaagacg gagggagtca gacattcagg agatcctcag
     2461 caagagagct gttcttgaag taacttaccc ctacagcagg cttttatttc aggttcctga
     2521 aaatttccgc atgaaacccc gcatcctcgt gatcgatgat gactcagcca tcttggagct
     2581 ggtcgccgtc aatctggaga tgtctggcta tgacgtacgc aaagctgagg acggcattaa
     2641 aggtcaggct ttagctgttc agctagttcc cgacctgatc atgctggatc taatgctgcc
     2701 gcgggttgat ggctttaccg tctgtcagcg actgcggcgc gatgagcgta ctgccgaaat
     2761 tccggtgctg atgctgaccg ccctcggaca gactcaggat aaggttgaag gcttcaacgc
     2821 gggtgctgac gattatctga ctaagccctt cgaagttgaa gagatgctgg cccgcgtgcg
     2881 tgccttgctg cagcgcaccg atcgcattcc ccatgcagcc cgccatagcg aaattctcag
     2941 ctacggtccg ctgaccctga ttcccgagcg gtttgaggcc atttggttca accgcacggt
     3001 caagctgact cacttggaat ttgagttgtt gcactgcctg ttgcaacgcc acggccaaac
     3061 ggttgcgccg agcgaaatcc tcaaagaagt ctggggctat gatcccgacg atgacatcga
     3121 gacgattcgc gtccacatcc gtcatctgcg caccaagctc gagcccgatc cccggcaccc
     3181 gcgctacatc aaaacggtct atggagcggg ctactgcctt gagctgccgg ccgagacgga
     3241 actccaccaa cacgccgatc agtttccttc ggcgtcctga acccaagcac tgagcagcac
     3301 ttcccagacg agtcgcggct gaacaaaggc tcgcagttgt ttctgtgcct gttcgagctg
     3361 gagcagttgc tgggactgca gctgttgctg ggaccagccc tgctgctgga ggtagcgaca
     3421 aagccaaagc tgggcttcga cgtcgaggcc agcatcgatc gcttgcgcca gttccaaagc
     3481 cacttgcaaa gagtttggcc gttgctgaag gcgatcgcgc aattctggcg
//
