LOCUS       sequence-19325-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf253
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             465..506
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             516..533
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(556..573)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    562..786
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatgaatcc caaacattgg
       61 ggaagagccg tatggaccat tatttttata gtactttcgc aagcgggttt agacggcaac
      121 atagaagcgt gtaaacgaaa actatatact atagttagca ctcttccatg tcctgcatgt
      181 agacggcacg cgactatcgc tatagaggac aataatgtca tgtctagcga tgatctgaat
      241 tatatttatt attttttcat cagattattt aacaatttgg catctgatcc caaatacgcg
      301 atcgctgtga caaaggttaa ccctttataa acttaaccca ttataaaact tatgattagt
      361 cacgactgaa gcaattcccg gggatccgaa taaagggtca agacaattct gcagatatcc
      421 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
      481 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
      541 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      601 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      661 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
      721 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
      781 ctatggcttc tgaggcggaa agaaccagct ggggctctag gggggtatcc cacgcgccct
      841 gtagcggcgc attaagcgcg gcgggt
//
