LOCUS       sequence-193258-        7739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses putative cMYC DBD with a HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN69
FEATURES             Location/Qualifiers
     source          1..7739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(526..528,529..531,532..1242)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1480..1509
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1570..1596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1570..1596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1606..2194
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1659..1679)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2077..2088)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2347..2527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2599..2720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2636..2655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2690..2709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2760..2895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2915..2934)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2916..2934)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2944..2961)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2944..2960)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2953..2975)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3102..3557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3189..3208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3399..3420
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3583..3687
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3688..3756,3757..4548)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3906..3925)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4719..5307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5208..5227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5461..5478
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5595..5616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5631..5636,5637..5654,5655..5661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5669..5685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5674..5696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5693..5709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5693..5709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5728..5748
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5730..5748
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5776..6002
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6003..6183
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6230..6355
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6848..7081
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7266..7310
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7577..7694
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      721 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1141 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactct cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat ggtcaagagg
     1321 cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag cttttttgcc
     1381 ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt agttatcctt
     1441 aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct catttctgaa
     1501 gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca gctacggaac
     1561 tcttgtgcgt acccctacga cgtgcccgac tacgcctaag tcgacaatca acctctggat
     1621 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     1681 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     1741 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     1801 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     1861 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     1921 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     1981 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     2041 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     2101 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     2161 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa
     2221 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     2281 tggaagggct aattcactcc caacgaagac aagatatcct gctagctccg gatccggacc
     2341 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     2401 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     2461 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     2521 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     2581 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2701 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     2761 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2821 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2881 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     2941 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3001 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3061 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     3121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3541 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3781 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3841 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3901 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3961 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4021 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4081 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4141 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4201 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4261 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4321 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4381 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4441 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4501 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4561 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4621 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4681 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4741 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4801 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4861 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4921 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4981 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5041 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5101 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5161 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5221 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5281 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5341 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5401 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5461 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5521 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5581 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggt tttacacttt
     5641 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     5701 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     5761 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     5821 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     5881 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     5941 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     6001 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6061 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6121 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6181 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     6241 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     6301 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     6361 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     6421 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     6481 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     6541 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     6601 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     6661 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     6721 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6781 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6841 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     6901 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     6961 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7021 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7081 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7141 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7201 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7261 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7321 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7381 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7441 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     7501 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     7561 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     7621 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     7681 aaattcaaaa ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattccac
//
