LOCUS       sequence-193262-        8624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric OCT4-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN21
FEATURES             Location/Qualifiers
     source          1..8624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(526..528,529..531,532..1242)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLFIQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2491..3079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2544..2564)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2962..2973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3232..3412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3484..3605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3521..3540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3575..3594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3645..3780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3800..3819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3801..3819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3829..3846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3829..3845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3838..3860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3987..4442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4074..4093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4284..4305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4468..4572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4573..4641,4642..5433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4791..4810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5604..6192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6093..6112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6346..6363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6480..6501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6516..6521,6522..6539,6540..6546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6554..6570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6559..6581
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6578..6594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6578..6594
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6613..6633
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6615..6633
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6661..6887
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6888..7068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7115..7240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7733..7966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8151..8195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8462..8579
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      721 agctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 tacaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggcgaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gttcatccag
     1141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactca cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat ggcgggacac
     1321 ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg gaggtgatgg gccagggggg
     1381 ccggagccgg gctgggttga tcctcggacc tggctaagct tccaaggccc tcctggaggg
     1441 ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt gggggattcc cccatgcccc
     1501 ccgccgtatg agttctgtgg ggggatggcg tactgtgggc cccaggttgg agtggggcta
     1561 gtgccccaag gcggcttgga gacctctcag cctgagggtg aagcaggagt cggggtggag
     1621 agcaactccg atggggcctc cccggagccc tgcaccgtca cccctggtgc cgtgaagctg
     1681 gagaaggaga agctggagca aaacccggag gagtcccagg acatcaaagc tctgcagaaa
     1741 gaactcgagc aatttgccaa gctcctgaag cagaagagga tcaccctggg atatacacag
     1801 gccgatgtgg ggctcaccct gggggttcta tttgggaagg tattcagcca aacgaccatc
     1861 tgccgctttg aggctctgca gcttagcttc aagaacatgt gtaagctgcg gcccttgctg
     1921 cagaagtggg tggaggaagc tgacaacaat gaaaatcttc aggagatatg caaagcagaa
     1981 accctcgtgc aggcccgaaa gagaaagcga accagtatcg agaaccgagt gagaggcaac
     2041 ctggagaatt tgttcctgca gtgcccgaaa cccacactgc agcagatcag ccacatcgcc
     2101 cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag
     2161 ggcaagcgat caagcagcga ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct
     2221 ttctcagggg gaccagtgtc ctttcctctg gccccagggc cccattttgg taccccaggc
     2281 tatgggagcc ctcacttcac tgcactgtac tcctcggtcc ctttccctga gggggaagcc
     2341 tttccccctg tctctgtcac cactctgggc tctcccatgc attcaaacca cttagacggc
     2401 gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg
     2461 gacggggatt ccccgggtcc gtgagtcgac aatcaacctc tggattacaa aatttgtgaa
     2521 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2581 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2641 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2701 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2761 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2821 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2881 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2941 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3001 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3061 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     3121 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3181 actcccaacg aagacaagat atcctgctag ctccggatcc ggacctgtac tgggtctctc
     3241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     3421 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     3481 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3541 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3601 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     3661 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3721 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3781 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     3841 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3901 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3961 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     4021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4201 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4381 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4441 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4561 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4621 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4681 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4741 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4801 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4861 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4921 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4981 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5041 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5101 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5161 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5221 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5281 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5341 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5401 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6301 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6361 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6421 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6481 aatgtgagtt agctcactca ttaggcaccc caggttttac actttatgct tccggctcgt
     6541 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6601 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     6661 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     6721 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     6781 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     6841 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     6901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     7081 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     7141 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     7201 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     7261 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     7321 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     7381 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7441 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7501 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7561 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7621 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7681 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7741 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7801 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7861 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7921 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7981 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     8041 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     8101 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     8161 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     8221 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     8281 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     8341 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     8401 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     8461 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     8521 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     8581 tcgatcacga gactagcctc gagaagcttg atatcgaatt ccac
//
