LOCUS       sequence-193264-        8729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric OCT4-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN20
FEATURES             Location/Qualifiers
     source          1..8729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(526..528,529..531,532..1242)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLFIQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2596..3184
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2649..2669)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3067..3078)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3337..3517
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3589..3710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3626..3645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3680..3699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3750..3885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3905..3924)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3906..3924)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3934..3951)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3934..3950)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3943..3965)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4092..4547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4179..4198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4389..4410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4573..4677
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4678..4746,4747..5538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4896..4915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5709..6297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6198..6217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6451..6468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6585..6606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6621..6626,6627..6644,6645..6651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6659..6675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6664..6686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6683..6699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6683..6699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6718..6738
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6720..6738
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6766..6992
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6993..7173
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7220..7345
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7838..8071
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8256..8300
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8567..8684
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      721 agctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 tacaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggcgaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gttcatccag
     1141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactca cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat ggcgggacac
     1321 ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg gaggtgatgg gccagggggg
     1381 ccggagccgg gctgggttga tcctcggacc tggctaagct tccaaggccc tcctggaggg
     1441 ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt gggggattcc cccatgcccc
     1501 ccgccgtatg agttctgtgg ggggatggcg tactgtgggc cccaggttgg agtggggcta
     1561 gtgccccaag gcggcttgga gacctctcag cctgagggtg aagcaggagt cggggtggag
     1621 agcaactccg atggggcctc cccggagccc tgcaccgtca cccctggtgc cgtgaagctg
     1681 gagaaggaga agctggagca aaacccggag gagtcccagg acatcaaagc tctgcagaaa
     1741 gaactcgagc aatttgccaa gctcctgaag cagaagagga tcaccctggg atatacacag
     1801 gccgatgtgg ggctcaccct gggggttcta tttgggaagg tattcagcca aacgaccatc
     1861 tgccgctttg aggctctgca gcttagcttc aagaacatgt gtaagctgcg gcccttgctg
     1921 cagaagtggg tggaggaagc tgacaacaat gaaaatcttc aggagatatg caaagcagaa
     1981 accctcgtgc aggcccgaaa gagaaagcga accagtatcg agaaccgagt gagaggcaac
     2041 ctggagaatt tgttcctgca gtgcccgaaa cccacactgc agcagatcag ccacatcgcc
     2101 cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag
     2161 ggcaagcgat caagcagcga ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct
     2221 ttctcagggg gaccagtgtc ctttcctctg gccccagggc cccattttgg taccccaggc
     2281 tatgggagcc ctcacttcac tgcactgtac tcctcggtcc ctttccctga gggggaagcc
     2341 tttccccctg tctctgtcac cactctgggc tctcccatgc attcaaacca cttagacggc
     2401 gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg
     2461 gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta cggcgctctg
     2521 gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac
     2581 ggtgggtgag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2641 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2701 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2761 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2821 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2881 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2941 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     3001 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3061 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3121 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3181 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     3241 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3301 aagatatcct gctagctccg gatccggacc tgtactgggt ctctctggtt agaccagatc
     3361 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3421 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3481 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     3541 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     3601 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3661 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3721 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3781 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3841 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     3901 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     3961 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4021 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4081 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4141 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4201 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4261 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4321 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4381 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4441 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4501 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     4561 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4621 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4681 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4741 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4801 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4861 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4921 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4981 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5041 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5101 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5161 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5221 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5281 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5341 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5401 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5461 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5521 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5581 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5641 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5701 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5761 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5821 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5881 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5941 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6001 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6061 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6121 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6181 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6241 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6301 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6361 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6421 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6481 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     6541 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     6601 actcattagg caccccaggt tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     6661 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     6721 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     6781 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     6841 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     6901 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     6961 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     7021 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7081 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7141 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     7201 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     7261 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     7321 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     7381 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     7441 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     7501 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     7561 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     7621 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     7681 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     7741 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     7801 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     7861 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     7921 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     7981 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8041 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     8101 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     8161 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     8221 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     8281 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     8341 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     8401 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     8461 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     8521 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     8581 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     8641 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta
     8701 gcctcgagaa gcttgatatc gaattccac
//
